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Book Chapter: Appendix C: Automated vitrification of mammalian embryos on a digital microfluidic device

TitleAppendix C: Automated vitrification of mammalian embryos on a digital microfluidic device
Authors
Liu, JunPyne, Derek G.Abdelgawad, MohamedSun, Yu
KeywordsAutomated vitrification
Digital microfluidics
Embryo vitrification
Issue Date2017
Citation
Methods in Molecular Biology, 2017, v. 1568, p. 309-316 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-4939-6828-2_23
AbstractThis chapter introduces a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual microdroplets manipulated on the microfluidic device were used as microvessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
Persistent Identifierhttp://hdl.handle.net/10722/349177
ISSN
1064-3745
2023 SCImago Journal Rankings: 0.399

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jun-
dc.contributor.authorPyne, Derek G.-
dc.contributor.authorAbdelgawad, Mohamed-
dc.contributor.authorSun, Yu-
dc.date.accessioned2024-10-17T06:56:47Z-
dc.date.available2024-10-17T06:56:47Z-
dc.date.issued2017-
dc.identifier.citationMethods in Molecular Biology, 2017, v. 1568, p. 309-316-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/349177-
dc.description.abstractThis chapter introduces a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual microdroplets manipulated on the microfluidic device were used as microvessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.-
dc.languageeng-
dc.relation.ispartofMethods in Molecular Biology-
dc.subjectAutomated vitrification-
dc.subjectDigital microfluidics-
dc.subjectEmbryo vitrification-
dc.titleAppendix C: Automated vitrification of mammalian embryos on a digital microfluidic device-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4939-6828-2_23-
dc.identifier.pmid28421507-
dc.identifier.scopuseid_2-s2.0-85018729544-
dc.identifier.volume1568-
dc.identifier.spage309-
dc.identifier.epage316-

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