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Article: Single-cell EpiChem jointly measures drug–chromatin binding and multimodal epigenome
Title | Single-cell EpiChem jointly measures drug–chromatin binding and multimodal epigenome |
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Authors | |
Issue Date | 1-Jan-2024 |
Publisher | Nature Research |
Citation | Nature Methods, 2024, v. 21, n. 9, p. 1624-1633 How to Cite? |
Abstract | Studies of molecular and cellular functions of small-molecule inhibitors in cancer treatment, eliciting effects by targeting genome and epigenome associated proteins, requires measurement of drug-target engagement in single-cell resolution. Here we present EpiChem for in situ single-cell joint mapping of small molecules and multimodal epigenomic landscape. We demonstrate single-cell co-assays of three small molecules together with histone modifications, chromatin accessibility or target proteins in human colorectal cancer (CRC) organoids. Integrated multimodal analysis reveals diverse drug interactions in the context of chromatin states within heterogeneous CRC organoids. We further reveal drug genomic binding dynamics and adaptive epigenome across cell types after small-molecule drug treatment in CRC organoids. This method provides a unique tool to exploit the mechanisms of cell type-specific drug actions. |
Persistent Identifier | http://hdl.handle.net/10722/348506 |
ISSN | 2023 Impact Factor: 36.1 2023 SCImago Journal Rankings: 14.796 |
DC Field | Value | Language |
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dc.contributor.author | Dong, Chao | - |
dc.contributor.author | Meng, Xiaoxuan | - |
dc.contributor.author | Zhang, Tong | - |
dc.contributor.author | Guo, Zhifang | - |
dc.contributor.author | Liu, Yaxi | - |
dc.contributor.author | Wu, Peihuang | - |
dc.contributor.author | Chen, Shiwei | - |
dc.contributor.author | Zhou, Fanqi | - |
dc.contributor.author | Ma, Yanni | - |
dc.contributor.author | Xiong, Haiqing | - |
dc.contributor.author | Shu, Shaokun | - |
dc.contributor.author | He, Aibin | - |
dc.date.accessioned | 2024-10-10T00:31:10Z | - |
dc.date.available | 2024-10-10T00:31:10Z | - |
dc.date.issued | 2024-01-01 | - |
dc.identifier.citation | Nature Methods, 2024, v. 21, n. 9, p. 1624-1633 | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | http://hdl.handle.net/10722/348506 | - |
dc.description.abstract | Studies of molecular and cellular functions of small-molecule inhibitors in cancer treatment, eliciting effects by targeting genome and epigenome associated proteins, requires measurement of drug-target engagement in single-cell resolution. Here we present EpiChem for in situ single-cell joint mapping of small molecules and multimodal epigenomic landscape. We demonstrate single-cell co-assays of three small molecules together with histone modifications, chromatin accessibility or target proteins in human colorectal cancer (CRC) organoids. Integrated multimodal analysis reveals diverse drug interactions in the context of chromatin states within heterogeneous CRC organoids. We further reveal drug genomic binding dynamics and adaptive epigenome across cell types after small-molecule drug treatment in CRC organoids. This method provides a unique tool to exploit the mechanisms of cell type-specific drug actions. | - |
dc.language | eng | - |
dc.publisher | Nature Research | - |
dc.relation.ispartof | Nature Methods | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Single-cell EpiChem jointly measures drug–chromatin binding and multimodal epigenome | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41592-024-02360-0 | - |
dc.identifier.scopus | eid_2-s2.0-85198933589 | - |
dc.identifier.volume | 21 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1624 | - |
dc.identifier.epage | 1633 | - |
dc.identifier.eissn | 1548-7105 | - |
dc.identifier.issnl | 1548-7091 | - |