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- Publisher Website: 10.3390/vaccines11101550
- Scopus: eid_2-s2.0-85175312371
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Article: An RNA-Scaffold Protein Subunit Vaccine for Nasal Immunization
Title | An RNA-Scaffold Protein Subunit Vaccine for Nasal Immunization |
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Authors | |
Keywords | mucosal immunity nasal vaccine protein subunit vaccine RNA scaffold SARS-CoV-2 self-adjuvanted |
Issue Date | 29-Sep-2023 |
Publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
Citation | Vaccines, 2023, v. 11, n. 10 How to Cite? |
Abstract | Developing recombinant proteins as nasal vaccines for inducing systemic and mucosal immunity against respiratory viruses is promising. However, additional adjuvants are required to overcome the low immunogenicity of protein antigens. Here, a self-adjuvanted protein-RNA ribonucleoprotein vaccine was developed and found to be an effective nasal vaccine in mice and the SARS-CoV-2 infection model. The vaccine consisted of spike RBD (as an antigen), nucleoprotein (as an adaptor), and ssRNA (as an adjuvant and RNA scaffold). This combination robustly induced mucosal IgA, neutralizing antibodies and activated multifunctional T-cells, while also providing sterilizing immunity against live virus challenge. In addition, high-resolution scRNA-seq analysis highlighted airway-resident immune cells profile during prime-boost immunization. The vaccine also possesses modularity (antigen/adaptor/RNA scaffold) and can be made to target other viruses. This protein-RNA ribonucleoprotein vaccine is a novel and promising approach for developing safe and potent nasal vaccines to combat respiratory virus infections. |
Persistent Identifier | http://hdl.handle.net/10722/347643 |
ISSN | 2023 Impact Factor: 5.2 2023 SCImago Journal Rankings: 1.201 |
DC Field | Value | Language |
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dc.contributor.author | Lam, Joy Yan | - |
dc.contributor.author | Wong, Wan Man | - |
dc.contributor.author | Yuen, Chun Kit | - |
dc.contributor.author | Ng, Yau Yee | - |
dc.contributor.author | San, Chun Hin | - |
dc.contributor.author | Yuen, Kwok Yung | - |
dc.contributor.author | Kok, Kin Hang | - |
dc.date.accessioned | 2024-09-26T00:30:20Z | - |
dc.date.available | 2024-09-26T00:30:20Z | - |
dc.date.issued | 2023-09-29 | - |
dc.identifier.citation | Vaccines, 2023, v. 11, n. 10 | - |
dc.identifier.issn | 2076-393X | - |
dc.identifier.uri | http://hdl.handle.net/10722/347643 | - |
dc.description.abstract | Developing recombinant proteins as nasal vaccines for inducing systemic and mucosal immunity against respiratory viruses is promising. However, additional adjuvants are required to overcome the low immunogenicity of protein antigens. Here, a self-adjuvanted protein-RNA ribonucleoprotein vaccine was developed and found to be an effective nasal vaccine in mice and the SARS-CoV-2 infection model. The vaccine consisted of spike RBD (as an antigen), nucleoprotein (as an adaptor), and ssRNA (as an adjuvant and RNA scaffold). This combination robustly induced mucosal IgA, neutralizing antibodies and activated multifunctional T-cells, while also providing sterilizing immunity against live virus challenge. In addition, high-resolution scRNA-seq analysis highlighted airway-resident immune cells profile during prime-boost immunization. The vaccine also possesses modularity (antigen/adaptor/RNA scaffold) and can be made to target other viruses. This protein-RNA ribonucleoprotein vaccine is a novel and promising approach for developing safe and potent nasal vaccines to combat respiratory virus infections. | - |
dc.language | eng | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.relation.ispartof | Vaccines | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | mucosal immunity | - |
dc.subject | nasal vaccine | - |
dc.subject | protein subunit vaccine | - |
dc.subject | RNA scaffold | - |
dc.subject | SARS-CoV-2 | - |
dc.subject | self-adjuvanted | - |
dc.title | An RNA-Scaffold Protein Subunit Vaccine for Nasal Immunization | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/vaccines11101550 | - |
dc.identifier.scopus | eid_2-s2.0-85175312371 | - |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 10 | - |
dc.identifier.issnl | 2076-393X | - |