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- Publisher Website: 10.1021/acs.langmuir.2c00341
- Scopus: eid_2-s2.0-85134083573
- PMID: 35748862
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Article: Quantification of Intracellular Proteins in Single Cells Based on Engineered Picoliter Droplets
Title | Quantification of Intracellular Proteins in Single Cells Based on Engineered Picoliter Droplets |
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Authors | |
Issue Date | 2022 |
Citation | Langmuir, 2022, v. 38, n. 26, p. 7929-7937 How to Cite? |
Abstract | Unlike conventional bulk measurements, single-cell protein analysis permits quantification of protein expression in individual cells. This has shed light on the cell-to-cell variation in heterogeneous biological systems, such as solid tumors, brain tissues, and developing embryos. Herein, a microfluidic method is developed to profile protein expression in individual cells by performing single-cell intracellular protein immunoassay in picoliter paired droplets. The high sensitivity of single-cell protein analysis on a chip is achieved by the confined reaction volume of picoliter droplets, efficient kinetic characteristics of the immunoassay through active mixing, and minimum single-cell protein loss by integrated operations. The abundance of an intracellular prostate specific antigen at the single-cell level is measured, and then the platform is applied to identify cell types and investigate heterogeneity within cell populations. Overall, a paired chip for single-cell immunoassay establishes a foundation for parallel, sensitive, and integrated protein quantification at the single-cell level and will find wide applications in the field of single-cell proteomics. |
Persistent Identifier | http://hdl.handle.net/10722/347204 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 0.786 |
DC Field | Value | Language |
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dc.contributor.author | Liu, Weizhi | - |
dc.contributor.author | Zhang, Ruihua | - |
dc.contributor.author | Huang, Shanqing | - |
dc.contributor.author | Li, Xingrui | - |
dc.contributor.author | Liu, Wanling | - |
dc.contributor.author | Zhou, Jianhui | - |
dc.contributor.author | Zhu, Lin | - |
dc.contributor.author | Song, Yanling | - |
dc.contributor.author | Yang, Chaoyong | - |
dc.date.accessioned | 2024-09-19T07:36:26Z | - |
dc.date.available | 2024-09-19T07:36:26Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Langmuir, 2022, v. 38, n. 26, p. 7929-7937 | - |
dc.identifier.issn | 0743-7463 | - |
dc.identifier.uri | http://hdl.handle.net/10722/347204 | - |
dc.description.abstract | Unlike conventional bulk measurements, single-cell protein analysis permits quantification of protein expression in individual cells. This has shed light on the cell-to-cell variation in heterogeneous biological systems, such as solid tumors, brain tissues, and developing embryos. Herein, a microfluidic method is developed to profile protein expression in individual cells by performing single-cell intracellular protein immunoassay in picoliter paired droplets. The high sensitivity of single-cell protein analysis on a chip is achieved by the confined reaction volume of picoliter droplets, efficient kinetic characteristics of the immunoassay through active mixing, and minimum single-cell protein loss by integrated operations. The abundance of an intracellular prostate specific antigen at the single-cell level is measured, and then the platform is applied to identify cell types and investigate heterogeneity within cell populations. Overall, a paired chip for single-cell immunoassay establishes a foundation for parallel, sensitive, and integrated protein quantification at the single-cell level and will find wide applications in the field of single-cell proteomics. | - |
dc.language | eng | - |
dc.relation.ispartof | Langmuir | - |
dc.title | Quantification of Intracellular Proteins in Single Cells Based on Engineered Picoliter Droplets | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/acs.langmuir.2c00341 | - |
dc.identifier.pmid | 35748862 | - |
dc.identifier.scopus | eid_2-s2.0-85134083573 | - |
dc.identifier.volume | 38 | - |
dc.identifier.issue | 26 | - |
dc.identifier.spage | 7929 | - |
dc.identifier.epage | 7937 | - |
dc.identifier.eissn | 1520-5827 | - |