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- Publisher Website: 10.1021/acsami.3c14983
- Scopus: eid_2-s2.0-85184861441
- PMID: 38295266
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Article: Multilayered Shape-Morphing Scaffolds with a Hierarchical Structure for Uterine Tissue Regeneration
Title | Multilayered Shape-Morphing Scaffolds with a Hierarchical Structure for Uterine Tissue Regeneration |
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Authors | |
Keywords | 3D bioprinting cell-laden hydrogel controlled release multilayer film shape morphing uterine tissue engineering |
Issue Date | 31-Jan-2024 |
Publisher | American Chemical Society |
Citation | ACS Applied Materials and Interfaces, 2024, v. 16, n. 6, p. 6772-6788 How to Cite? |
Abstract | Owing to dysfunction of the uterus, millions of couples around the world suffer from infertility. Different from conventional treatments, tissue engineering provides a new and promising approach to deal with difficult problems such as human tissue or organ failure. Adopting scaffold-based tissue engineering, three-dimensional (3D) porous scaffolds in combination with stem cells and appropriate biomolecules may be constructed for uterine tissue regeneration. In this study, a hierarchical tissue engineering scaffold, which mimicked the uterine tissue structure and functions, was designed, and the biomimicking scaffolds were then successfully fabricated using solvent casting, layer-by-layer assembly, and 3D bioprinting techniques. For the multilayered, hierarchical structured scaffolds, poly(l-lactide-co-trimethylene carbonate) (PLLA-co-TMC, “PLATMC” in short) and poly(lactic acid-co-glycolic acid) (PLGA) blends were first used to fabricate the shape-morphing layer of the scaffolds, which was to mimic the function of myometrium in uterine tissue. The PLATMC/PLGA polymer blend scaffolds were highly stretchable. Subsequently, after etching of the PLATMC/PLGA surface and employing estradiol (E2), polydopamine (PDA), and hyaluronic acid (HA), PDA@E2/HA multilayer films were formed on PLATMC/PLGA scaffolds to build an intelligent delivery platform to enable controlled and sustained release of E2. The PDA@E2/HA multilayer films also improved the biological performance of the scaffold. Finally, a layer of bone marrow-derived mesenchymal stem cell (BMSC)-laden hydrogel [which was a blend of gelatin methacryloyl (GelMA) and gelatin (Gel)] was 3D printed on the PDA@E2/HA multilayer films of the scaffold, thereby completing the construction of the hierarchical scaffold. BMSCs in the GelMA/Gel hydrogel layer exhibited excellent cell viability and could spread and be released eventually upon biodegradation of the GelMA/Gel hydrogel. It was shown that the hierarchically structured scaffolds could evolve from the initial flat shape into the tubular structure completely in an aqueous environment at 37 °C, fulfilling the requirement for curved scaffolds for uterine tissue engineering. The biomimicking scaffolds with a hierarchical structure and curved shape, high stretchability, and controlled and sustained E2 release appear to be very promising for uterine tissue regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/344755 |
ISSN | 2023 Impact Factor: 8.3 2023 SCImago Journal Rankings: 2.058 |
DC Field | Value | Language |
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dc.contributor.author | Chen, Shangsi | - |
dc.contributor.author | Tan, Shenglong | - |
dc.contributor.author | Zheng, Liwu | - |
dc.contributor.author | Wang, Min | - |
dc.date.accessioned | 2024-08-06T08:46:40Z | - |
dc.date.available | 2024-08-06T08:46:40Z | - |
dc.date.issued | 2024-01-31 | - |
dc.identifier.citation | ACS Applied Materials and Interfaces, 2024, v. 16, n. 6, p. 6772-6788 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | http://hdl.handle.net/10722/344755 | - |
dc.description.abstract | <p>Owing to dysfunction of the uterus, millions of couples around the world suffer from infertility. Different from conventional treatments, tissue engineering provides a new and promising approach to deal with difficult problems such as human tissue or organ failure. Adopting scaffold-based tissue engineering, three-dimensional (3D) porous scaffolds in combination with stem cells and appropriate biomolecules may be constructed for uterine tissue regeneration. In this study, a hierarchical tissue engineering scaffold, which mimicked the uterine tissue structure and functions, was designed, and the biomimicking scaffolds were then successfully fabricated using solvent casting, layer-by-layer assembly, and 3D bioprinting techniques. For the multilayered, hierarchical structured scaffolds, poly(l-lactide-co-trimethylene carbonate) (PLLA-co-TMC, “PLATMC” in short) and poly(lactic acid-co-glycolic acid) (PLGA) blends were first used to fabricate the shape-morphing layer of the scaffolds, which was to mimic the function of myometrium in uterine tissue. The PLATMC/PLGA polymer blend scaffolds were highly stretchable. Subsequently, after etching of the PLATMC/PLGA surface and employing estradiol (E2), polydopamine (PDA), and hyaluronic acid (HA), PDA@E2/HA multilayer films were formed on PLATMC/PLGA scaffolds to build an intelligent delivery platform to enable controlled and sustained release of E2. The PDA@E2/HA multilayer films also improved the biological performance of the scaffold. Finally, a layer of bone marrow-derived mesenchymal stem cell (BMSC)-laden hydrogel [which was a blend of gelatin methacryloyl (GelMA) and gelatin (Gel)] was 3D printed on the PDA@E2/HA multilayer films of the scaffold, thereby completing the construction of the hierarchical scaffold. BMSCs in the GelMA/Gel hydrogel layer exhibited excellent cell viability and could spread and be released eventually upon biodegradation of the GelMA/Gel hydrogel. It was shown that the hierarchically structured scaffolds could evolve from the initial flat shape into the tubular structure completely in an aqueous environment at 37 °C, fulfilling the requirement for curved scaffolds for uterine tissue engineering. The biomimicking scaffolds with a hierarchical structure and curved shape, high stretchability, and controlled and sustained E2 release appear to be very promising for uterine tissue regeneration.</p> | - |
dc.language | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.ispartof | ACS Applied Materials and Interfaces | - |
dc.subject | 3D bioprinting | - |
dc.subject | cell-laden hydrogel | - |
dc.subject | controlled release | - |
dc.subject | multilayer film | - |
dc.subject | shape morphing | - |
dc.subject | uterine tissue engineering | - |
dc.title | Multilayered Shape-Morphing Scaffolds with a Hierarchical Structure for Uterine Tissue Regeneration | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsami.3c14983 | - |
dc.identifier.pmid | 38295266 | - |
dc.identifier.scopus | eid_2-s2.0-85184861441 | - |
dc.identifier.volume | 16 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 6772 | - |
dc.identifier.epage | 6788 | - |
dc.identifier.eissn | 1944-8252 | - |
dc.identifier.issnl | 1944-8244 | - |