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Article: Protocol for semisynthesis of serotonylated histone H3 by rapid protein desulfurization in tandem with native chemical ligation
| Title | Protocol for semisynthesis of serotonylated histone H3 by rapid protein desulfurization in tandem with native chemical ligation |
|---|---|
| Authors | |
| Keywords | Chemistry Molecular/Chemical Probes Protein Biochemistry |
| Issue Date | 17-Mar-2023 |
| Publisher | Cell Press |
| Citation | STAR Protocols, 2023, v. 4, n. 1 How to Cite? |
| Abstract | Here, we present a protocol of rapid protein desulfurization in tandem with native chemical ligation for facile syntheses of proteins with site-specific modifications. We describe using sodium tetraethylborate (NaBEt4) to carry out this desulfurization in an add-and-done manner under ambient conditions without requirement of inert atmosphere protection, UV irradiation, heating, or exogenous thiol additives. Specifically, we detail the semisynthesis of serotonylated histone H3(H3Q5ser) via one-pot ligation desulfurization. This protocol can be applied to synthesize proteins of interest with homogenous post-translational modifications. For complete information on the generation and use of this protocol, please refer to Sun et al. (2022). |
| Persistent Identifier | http://hdl.handle.net/10722/344675 |
| ISSN | 2023 Impact Factor: 1.3 2023 SCImago Journal Rankings: 0.955 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Sun, Zhenquan | - |
| dc.contributor.author | Wei, Tongyao | - |
| dc.contributor.author | Cao, Yihui | - |
| dc.contributor.author | Li, Xuechen | - |
| dc.date.accessioned | 2024-07-31T06:22:57Z | - |
| dc.date.available | 2024-07-31T06:22:57Z | - |
| dc.date.issued | 2023-03-17 | - |
| dc.identifier.citation | STAR Protocols, 2023, v. 4, n. 1 | - |
| dc.identifier.issn | 2666-1667 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/344675 | - |
| dc.description.abstract | <p>Here, we present a protocol of rapid protein desulfurization in tandem with native chemical ligation for facile syntheses of proteins with site-specific modifications. We describe using sodium tetraethylborate (NaBEt<sub>4</sub>) to carry out this desulfurization in an add-and-done manner under ambient conditions without requirement of inert atmosphere protection, UV irradiation, heating, or exogenous thiol additives. Specifically, we detail the semisynthesis of serotonylated histone H3(H3Q5ser) <em>via</em> one-pot ligation desulfurization. This protocol can be applied to synthesize proteins of interest with homogenous post-translational modifications.</p><p>For complete information on the generation and use of this protocol, please refer to Sun et al. (2022).</p> | - |
| dc.language | eng | - |
| dc.publisher | Cell Press | - |
| dc.relation.ispartof | STAR Protocols | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.subject | Chemistry | - |
| dc.subject | Molecular/Chemical Probes | - |
| dc.subject | Protein Biochemistry | - |
| dc.title | Protocol for semisynthesis of serotonylated histone H3 by rapid protein desulfurization in tandem with native chemical ligation | - |
| dc.type | Article | - |
| dc.description.nature | published_or_final_version | - |
| dc.identifier.doi | 10.1016/j.xpro.2022.102042 | - |
| dc.identifier.scopus | eid_2-s2.0-85146454238 | - |
| dc.identifier.volume | 4 | - |
| dc.identifier.issue | 1 | - |
| dc.identifier.eissn | 2666-1667 | - |
| dc.identifier.issnl | 2666-1667 | - |