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Article: Identification and Measurement of Biomarkers at Single Microorganism Level for In Situ Monitoring Deep Ultraviolet Disinfection Process

TitleIdentification and Measurement of Biomarkers at Single Microorganism Level for In Situ Monitoring Deep Ultraviolet Disinfection Process
Authors
Keywordsadenovirus
Atomic force microscopy
deep ultraviolet irradiation
living cell
mechanical properties
Issue Date1-Apr-2024
PublisherInstitute of Electrical and Electronics Engineers
Citation
IEEE Transactions on NanoBioscience, 2024, v. 23, n. 2, p. 242-251 How to Cite?
Abstract
Since the COVID-19 disease has been further aggravated, the prevention of pathogen transmission becomes a vital issue to restrain casualties. Recent research outcomes have shown the possibilities of the viruses existing on inanimate surfaces up to few days, which carry the risk of touch propagation of the disease. Deep ultraviolet germicide irradiation (UVGI) with the wavelength of 255–280nm has been verified to efficiently disinfect various types of bacteria and virus, which could prevent the aggravation of pandemic spread. Even though considerable experiments and approaches have been applied to evaluate the disinfection effects, there are only few reports about how the individual bio-organism behaves after ultraviolet C (UVC) irradiation, especially in the aspect of mechanical changes. Furthermore, since the standard pathway of virus transmission and reproduction requires the host cell to assemble and transport newly generated virus, the dynamic response of infectious cell is always the vital aspect of virology study. In this work, high power LEDs array has been established with 270nm UVC irradiation to evaluate disinfection capability on various types of bio-organism, and incubator embedded atomic force microscopy (AFM) is used to investigate the single bacterium and virus under UVGI. The real-time tracking of the living Vero cells infected with adenovirus has also been presented in this study. The results show that after sufficient UVGI, the outer shell of bacteria and viruses remain intact in structure, however the bio-organisms lost the capability of reproduction and normal metabolism. The experiment results also indicate that once the host cell is infected with adenovirus, the rapid production of newborn virus capsid will gradually destroy the cellular normal metabolism and lose mechanical integrity.

Persistent Identifierhttp://hdl.handle.net/10722/343543
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 0.659

 

DC FieldValueLanguage
dc.contributor.authorXue, Yuxuan-
dc.contributor.authorMa, Ye-
dc.contributor.authorSun, Zhiyong-
dc.contributor.authorLiu, Xinyu-
dc.contributor.authorZhang, Mukun-
dc.contributor.authorZhang, Jiawei-
dc.contributor.authorXi, Ning-
dc.date.accessioned2024-05-14T05:21:20Z-
dc.date.available2024-05-14T05:21:20Z-
dc.date.issued2024-04-01-
dc.identifier.citationIEEE Transactions on NanoBioscience, 2024, v. 23, n. 2, p. 242-251-
dc.identifier.issn1536-1241-
dc.identifier.urihttp://hdl.handle.net/10722/343543-
dc.description.abstract<div>Since the COVID-19 disease has been further aggravated, the prevention of pathogen transmission becomes a vital issue to restrain casualties. Recent research outcomes have shown the possibilities of the viruses existing on inanimate surfaces up to few days, which carry the risk of touch propagation of the disease. Deep ultraviolet germicide irradiation (UVGI) with the wavelength of 255–280nm has been verified to efficiently disinfect various types of bacteria and virus, which could prevent the aggravation of pandemic spread. Even though considerable experiments and approaches have been applied to evaluate the disinfection effects, there are only few reports about how the individual bio-organism behaves after ultraviolet C (UVC) irradiation, especially in the aspect of mechanical changes. Furthermore, since the standard pathway of virus transmission and reproduction requires the host cell to assemble and transport newly generated virus, the dynamic response of infectious cell is always the vital aspect of virology study. In this work, high power LEDs array has been established with 270nm UVC irradiation to evaluate disinfection capability on various types of bio-organism, and incubator embedded atomic force microscopy (AFM) is used to investigate the single bacterium and virus under UVGI. The real-time tracking of the living Vero cells infected with adenovirus has also been presented in this study. The results show that after sufficient UVGI, the outer shell of bacteria and viruses remain intact in structure, however the bio-organisms lost the capability of reproduction and normal metabolism. The experiment results also indicate that once the host cell is infected with adenovirus, the rapid production of newborn virus capsid will gradually destroy the cellular normal metabolism and lose mechanical integrity.</div>-
dc.languageeng-
dc.publisherInstitute of Electrical and Electronics Engineers-
dc.relation.ispartofIEEE Transactions on NanoBioscience-
dc.subjectadenovirus-
dc.subjectAtomic force microscopy-
dc.subjectdeep ultraviolet irradiation-
dc.subjectliving cell-
dc.subjectmechanical properties-
dc.titleIdentification and Measurement of Biomarkers at Single Microorganism Level for In Situ Monitoring Deep Ultraviolet Disinfection Process-
dc.typeArticle-
dc.identifier.doi10.1109/TNB.2023.3312754-
dc.identifier.scopuseid_2-s2.0-85171583251-
dc.identifier.volume23-
dc.identifier.issue2-
dc.identifier.spage242-
dc.identifier.epage251-
dc.identifier.eissn1558-2639-
dc.identifier.issnl1536-1241-

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