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Article: Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale
Title | Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale |
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Authors | |
Keywords | Dendrobium officinale polysaccharide Oligosaccharide Structure analysis β-1,4-Mannan |
Issue Date | 2023 |
Citation | Food Hydrocolloids, 2023, v. 134, article no. 108038 How to Cite? |
Abstract | The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data. |
Persistent Identifier | http://hdl.handle.net/10722/343388 |
ISSN | 2023 Impact Factor: 11.0 2023 SCImago Journal Rankings: 2.717 |
DC Field | Value | Language |
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dc.contributor.author | Wong, Tin Long | - |
dc.contributor.author | Li, Li Feng | - |
dc.contributor.author | Zhang, Ji Xia | - |
dc.contributor.author | Zhang, Quan Wei | - |
dc.contributor.author | Zhang, Xue Ting | - |
dc.contributor.author | Zhou, Li Shuang | - |
dc.contributor.author | Fung, Hau Yee | - |
dc.contributor.author | Feng, Lei | - |
dc.contributor.author | Cheng, Hui Yuan | - |
dc.contributor.author | Huo, Chu Ying | - |
dc.contributor.author | Liu, Man | - |
dc.contributor.author | Bao, Wan Rong | - |
dc.contributor.author | Wu, Wen Jie | - |
dc.contributor.author | Lai, Cheuk Hei | - |
dc.contributor.author | Bai, Su Ping | - |
dc.contributor.author | Nie, Shao Ping | - |
dc.contributor.author | Puno, Pema Tenzin | - |
dc.contributor.author | Lau, Clara Bik San | - |
dc.contributor.author | Leung, Ping Chung | - |
dc.contributor.author | Han, Quan Bin | - |
dc.contributor.author | Sun, Han Dong | - |
dc.date.accessioned | 2024-05-10T09:07:42Z | - |
dc.date.available | 2024-05-10T09:07:42Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Food Hydrocolloids, 2023, v. 134, article no. 108038 | - |
dc.identifier.issn | 0268-005X | - |
dc.identifier.uri | http://hdl.handle.net/10722/343388 | - |
dc.description.abstract | The backbone of Dendrobium officinale polysaccharide (DOP) was reported to be glucomannan. But the dispute continues due to the limitation of conventional analysis methods (e.g., the overlapped NMR signals). Herein, we describe an oligosaccharide sequencing analysis that successfully corrects the backbone to mannan. This analysis involves producing oligosaccharide fragments from mild acid hydrolysis of DOP followed by p-aminobenzoic ethyl ester (ABEE) derivatization. Then, a series of oligosaccharides with varied polymerization degrees (DP = 2–10) were isolated and identified by LC-MS profiling, monosaccharide composition analysis, linkage analysis, and 1D-/2D-NMR elucidation. Finally, we conclude that the backbone of DOP is a β-D-1,4-mannan which is confirmed by other analysis such as comparison with reference mannan. This oligosaccharide analysis is more straightforward and reliable by transforming the complicated polymers into oligomers which have more accurate and convincing MS and NMR data. | - |
dc.language | eng | - |
dc.relation.ispartof | Food Hydrocolloids | - |
dc.subject | Dendrobium officinale polysaccharide | - |
dc.subject | Oligosaccharide | - |
dc.subject | Structure analysis | - |
dc.subject | β-1,4-Mannan | - |
dc.title | Oligosaccharide analysis of the backbone structure of the characteristic polysaccharide of Dendrobium officinale | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.foodhyd.2022.108038 | - |
dc.identifier.scopus | eid_2-s2.0-85135867905 | - |
dc.identifier.volume | 134 | - |
dc.identifier.spage | article no. 108038 | - |
dc.identifier.epage | article no. 108038 | - |