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Article: Alterations in gut microbiota of esophageal squamous cell carcinoma patients

TitleAlterations in gut microbiota of esophageal squamous cell carcinoma patients
Authors
Keywordsbiomarkers
dysbiosis
esophageal neoplasms
esophageal squamous cell carcinoma
gastrointestinal microbiome
Issue Date2022
Citation
Journal of Gastroenterology and Hepatology (Australia), 2022, v. 37, n. 10, p. 1919-1927 How to Cite?
AbstractBackground and Aim: Esophageal squamous cell carcinoma (ESCC) is the most common histological subtype of esophageal cancer worldwide. Patients with ESCC display an altered esophageal microbiota compared with healthy individuals; however, little is known about the gut microbiota in ESCC. Methods: Here, we characterized the fecal microbiota of 15 ESCC patients and 16 healthy control subjects using 16S rRNA gene sequencing. Results: After controlling for potential confounders, significant alterations in both taxonomic and functional composition of the gut microbiota in ESCC patients were observed. By contrast, alpha diversity of the gut microbiota did not significantly differ between the cases and controls. We observed an enrichment of potentially pro-inflammatory and/or carcinogenic bacteria, such as Butyricimonas, Veillonella, and Streptococcus, and a depletion of butyrate-producing and/or potentially anti-inflammatory bacteria, such as Butyricicoccus, Lachnospiraceae NK4A136 group, and Eubacterium eligens group, in the gut microbiota of ESCC patients. The log-ratios of Streptococcus to Butyricicoccus and Streptococcus to Lachnospiraceae NK4A136 group of the gut microbiota were identified as potential diagnostic biomarkers for ESCC, with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.863 (95% confidence interval: 0.707–1.000) and 0.825 (0.673–0.977), respectively. The diagnostic performance of both microbial biomarkers was validated in another ESCC cohort. Conclusions: This pilot study has revealed an altered gut microbiota in ESCC patients and has paved the way for large-scale prospective cohort studies to examine the causative relationship between ESCC and gut dysbiosis.
Persistent Identifierhttp://hdl.handle.net/10722/343383
ISSN
2023 Impact Factor: 3.7
2023 SCImago Journal Rankings: 1.179

 

DC FieldValueLanguage
dc.contributor.authorCheung, Man Kit-
dc.contributor.authorYue, Grace Gar Lee-
dc.contributor.authorLauw, Susana-
dc.contributor.authorLi, Cindy Shin Yee-
dc.contributor.authorYung, Man Yee-
dc.contributor.authorNg, Siew Chien-
dc.contributor.authorYip, Hon Chi-
dc.contributor.authorKwan, Hoi Shan-
dc.contributor.authorChiu, Philip Wai Yan-
dc.contributor.authorLau, Clara Bik San-
dc.date.accessioned2024-05-10T09:07:39Z-
dc.date.available2024-05-10T09:07:39Z-
dc.date.issued2022-
dc.identifier.citationJournal of Gastroenterology and Hepatology (Australia), 2022, v. 37, n. 10, p. 1919-1927-
dc.identifier.issn0815-9319-
dc.identifier.urihttp://hdl.handle.net/10722/343383-
dc.description.abstractBackground and Aim: Esophageal squamous cell carcinoma (ESCC) is the most common histological subtype of esophageal cancer worldwide. Patients with ESCC display an altered esophageal microbiota compared with healthy individuals; however, little is known about the gut microbiota in ESCC. Methods: Here, we characterized the fecal microbiota of 15 ESCC patients and 16 healthy control subjects using 16S rRNA gene sequencing. Results: After controlling for potential confounders, significant alterations in both taxonomic and functional composition of the gut microbiota in ESCC patients were observed. By contrast, alpha diversity of the gut microbiota did not significantly differ between the cases and controls. We observed an enrichment of potentially pro-inflammatory and/or carcinogenic bacteria, such as Butyricimonas, Veillonella, and Streptococcus, and a depletion of butyrate-producing and/or potentially anti-inflammatory bacteria, such as Butyricicoccus, Lachnospiraceae NK4A136 group, and Eubacterium eligens group, in the gut microbiota of ESCC patients. The log-ratios of Streptococcus to Butyricicoccus and Streptococcus to Lachnospiraceae NK4A136 group of the gut microbiota were identified as potential diagnostic biomarkers for ESCC, with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.863 (95% confidence interval: 0.707–1.000) and 0.825 (0.673–0.977), respectively. The diagnostic performance of both microbial biomarkers was validated in another ESCC cohort. Conclusions: This pilot study has revealed an altered gut microbiota in ESCC patients and has paved the way for large-scale prospective cohort studies to examine the causative relationship between ESCC and gut dysbiosis.-
dc.languageeng-
dc.relation.ispartofJournal of Gastroenterology and Hepatology (Australia)-
dc.subjectbiomarkers-
dc.subjectdysbiosis-
dc.subjectesophageal neoplasms-
dc.subjectesophageal squamous cell carcinoma-
dc.subjectgastrointestinal microbiome-
dc.titleAlterations in gut microbiota of esophageal squamous cell carcinoma patients-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jgh.15941-
dc.identifier.pmid35816164-
dc.identifier.scopuseid_2-s2.0-85134188260-
dc.identifier.volume37-
dc.identifier.issue10-
dc.identifier.spage1919-
dc.identifier.epage1927-
dc.identifier.eissn1440-1746-

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