File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Antidiabetic Effect of Schisandrae Chinensis Fructus Involves Inhibition of the Sodium Glucose Cotransporter

TitleAntidiabetic Effect of Schisandrae Chinensis Fructus Involves Inhibition of the Sodium Glucose Cotransporter
Authors
Keywordsdiabetes mellitus
Schisandrae Chinensis Fructus
SGLT
traditional Chinese medicine
Issue Date2015
Citation
Drug Development Research, 2015, v. 76, n. 1, p. 1-8 How to Cite?
Abstract(Table presented.). Schisandrae Chinensis Fructus (SCF), the fruit of Schisandra chinensis (Turcz.) Baill. (family Schisandraceae) is traditionally used as a tonic and antidiabetic agent in Asia. In this study, SCF was investigated for its effects on sodium glucose cotransporters 1 and 2 (SGLT 1 and 2) expressed in a COS-7 cell line for its specificity in inhibiting SGLT2, which is a novel mechanism to screen for potential antidiabetic agents. Using a bioassay-guided fractionation, we then tried to isolate and identify the active fraction(s)/component(s). The ethanol extract of SCF at a concentration of 1 mg/mL significantly inhibited 89% of SGLT1 and 73% of SGLT2 activities in a [14C]-α-methyl-d-glucopyranoside ([14C]-AMG) uptake assay. Fractionation of the ethanol extract yielded nine fractions, of which F8, at a concentration of 1 mg/mL, was specific in inhibiting SGLT 2 (42% inhibition, P < 0.001), without inhibiting SGLT 1. Using LC/MS-MS, three compounds, deoxyschisandrin, schisandrin B (γ-schisandrin) and schisandrin were identified in F8 and their amounts quantified. However, subsequent evaluation in the [14C]-AMG uptake assay showed that these three compounds failed to inhibit SGLT 2 activity indicating that the SGLT active component(s) from SCF have yet to be identified. Drug Dev Res 76 : 1–8, 2015.
Persistent Identifierhttp://hdl.handle.net/10722/343237
ISSN
2023 Impact Factor: 3.5
2023 SCImago Journal Rankings: 0.672

 

DC FieldValueLanguage
dc.contributor.authorQu, Yue-
dc.contributor.authorChan, Judy Yuet Wa-
dc.contributor.authorWong, Chun Wai-
dc.contributor.authorCheng, Ling-
dc.contributor.authorXu, Chuanshan-
dc.contributor.authorLeung, Albert Wing Nang-
dc.contributor.authorLau, Clara Bik San-
dc.date.accessioned2024-05-10T09:06:31Z-
dc.date.available2024-05-10T09:06:31Z-
dc.date.issued2015-
dc.identifier.citationDrug Development Research, 2015, v. 76, n. 1, p. 1-8-
dc.identifier.issn0272-4391-
dc.identifier.urihttp://hdl.handle.net/10722/343237-
dc.description.abstract(Table presented.). Schisandrae Chinensis Fructus (SCF), the fruit of Schisandra chinensis (Turcz.) Baill. (family Schisandraceae) is traditionally used as a tonic and antidiabetic agent in Asia. In this study, SCF was investigated for its effects on sodium glucose cotransporters 1 and 2 (SGLT 1 and 2) expressed in a COS-7 cell line for its specificity in inhibiting SGLT2, which is a novel mechanism to screen for potential antidiabetic agents. Using a bioassay-guided fractionation, we then tried to isolate and identify the active fraction(s)/component(s). The ethanol extract of SCF at a concentration of 1 mg/mL significantly inhibited 89% of SGLT1 and 73% of SGLT2 activities in a [14C]-α-methyl-d-glucopyranoside ([14C]-AMG) uptake assay. Fractionation of the ethanol extract yielded nine fractions, of which F8, at a concentration of 1 mg/mL, was specific in inhibiting SGLT 2 (42% inhibition, P < 0.001), without inhibiting SGLT 1. Using LC/MS-MS, three compounds, deoxyschisandrin, schisandrin B (γ-schisandrin) and schisandrin were identified in F8 and their amounts quantified. However, subsequent evaluation in the [14C]-AMG uptake assay showed that these three compounds failed to inhibit SGLT 2 activity indicating that the SGLT active component(s) from SCF have yet to be identified. Drug Dev Res 76 : 1–8, 2015.-
dc.languageeng-
dc.relation.ispartofDrug Development Research-
dc.subjectdiabetes mellitus-
dc.subjectSchisandrae Chinensis Fructus-
dc.subjectSGLT-
dc.subjecttraditional Chinese medicine-
dc.titleAntidiabetic Effect of Schisandrae Chinensis Fructus Involves Inhibition of the Sodium Glucose Cotransporter-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ddr.21233-
dc.identifier.scopuseid_2-s2.0-85015837935-
dc.identifier.volume76-
dc.identifier.issue1-
dc.identifier.spage1-
dc.identifier.epage8-
dc.identifier.eissn1098-2299-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats