File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Anti-Inflammatory Activities of Pentaherbs Formula, Berberine, Gallic Acid and Chlorogenic Acid in Atopic Dermatitis-Like Skin Inflammation

TitleAnti-Inflammatory Activities of Pentaherbs Formula, Berberine, Gallic Acid and Chlorogenic Acid in Atopic Dermatitis-Like Skin Inflammation
Authors
KeywordsAllergic inflammation
Atopic dermatitis
Berberine
Gallic acid
Pentaherbs formula
Issue Date2016
Citation
Molecules, 2016, v. 21, n. 4, article no. 519 How to Cite?
AbstractAtopic dermatitis (AD) is a common allergic skin disease, characterized by dryness, itchiness, thickening and inflammation of the skin. Infiltration of eosinophils into the dermal layer and presence of edema are typical characteristics in the skin biopsy of AD patients. Previous in vitro and clinical studies showed that the Pentaherbs formula (PHF) consisting of five traditional Chinese herbal medicines, Flos Lonicerae, Herba Menthae, Cortex Phellodendri, Cortex Moutan and Rhizoma Atractylodis at w/w ratio of 2:1:2:2:2 exhibited therapeutic potential in treating AD. In this study, an in vivo murine model with oxazolone (OXA)-mediated dermatitis was used to elucidate the efficacy of PHF. Active ingredients of PHF water extract were also identified and quantified, and their in vitro anti-inflammatory activities on pruritogenic cytokine IL-31- and alarmin IL-33-activated human eosinophils and dermal fibroblasts were evaluated. Ear swelling, epidermis thickening and eosinophils infiltration in epidermal and dermal layers, and the release of serum IL-12 of the murine OXA-mediated dermatitis were significantly reduced upon oral or topical treatment with PHF (all ρ < 0.05). Gallic acid, chlorogenic acid and berberine contents (w/w) in PHF were found to be 0.479%, 1.201% and 0.022%, respectively. Gallic acid and chlorogenic acid could suppress the release of pro-inflammatory cytokine IL-6 and chemokine CCL7 and CXCL8, respectively, in IL-31- and IL-33-treated eosinophils-dermal fibroblasts co-culture; while berberine could suppress the release of IL-6, CXCL8, CCL2 and CCL7 in the eosinophil culture and eosinophils-dermal fibroblasts co-culture (all ρ < 0.05). These findings suggest that PHF can ameliorate allergic inflammation and attenuate the activation of eosinophils.
Persistent Identifierhttp://hdl.handle.net/10722/343213

 

DC FieldValueLanguage
dc.contributor.authorTsang, Miranda S.M.-
dc.contributor.authorJiao, Delong-
dc.contributor.authorChan, Ben C.L.-
dc.contributor.authorHon, Kam Lun-
dc.contributor.authorLeung, Ping C.-
dc.contributor.authorLau, Clara B.S.-
dc.contributor.authorWong, Eric C.W.-
dc.contributor.authorCheng, Ling-
dc.contributor.authorChan, Carmen K.M.-
dc.contributor.authorLam, Christopher W.K.-
dc.contributor.authorWong, Chun K.-
dc.date.accessioned2024-05-10T09:06:21Z-
dc.date.available2024-05-10T09:06:21Z-
dc.date.issued2016-
dc.identifier.citationMolecules, 2016, v. 21, n. 4, article no. 519-
dc.identifier.urihttp://hdl.handle.net/10722/343213-
dc.description.abstractAtopic dermatitis (AD) is a common allergic skin disease, characterized by dryness, itchiness, thickening and inflammation of the skin. Infiltration of eosinophils into the dermal layer and presence of edema are typical characteristics in the skin biopsy of AD patients. Previous in vitro and clinical studies showed that the Pentaherbs formula (PHF) consisting of five traditional Chinese herbal medicines, Flos Lonicerae, Herba Menthae, Cortex Phellodendri, Cortex Moutan and Rhizoma Atractylodis at w/w ratio of 2:1:2:2:2 exhibited therapeutic potential in treating AD. In this study, an in vivo murine model with oxazolone (OXA)-mediated dermatitis was used to elucidate the efficacy of PHF. Active ingredients of PHF water extract were also identified and quantified, and their in vitro anti-inflammatory activities on pruritogenic cytokine IL-31- and alarmin IL-33-activated human eosinophils and dermal fibroblasts were evaluated. Ear swelling, epidermis thickening and eosinophils infiltration in epidermal and dermal layers, and the release of serum IL-12 of the murine OXA-mediated dermatitis were significantly reduced upon oral or topical treatment with PHF (all ρ < 0.05). Gallic acid, chlorogenic acid and berberine contents (w/w) in PHF were found to be 0.479%, 1.201% and 0.022%, respectively. Gallic acid and chlorogenic acid could suppress the release of pro-inflammatory cytokine IL-6 and chemokine CCL7 and CXCL8, respectively, in IL-31- and IL-33-treated eosinophils-dermal fibroblasts co-culture; while berberine could suppress the release of IL-6, CXCL8, CCL2 and CCL7 in the eosinophil culture and eosinophils-dermal fibroblasts co-culture (all ρ < 0.05). These findings suggest that PHF can ameliorate allergic inflammation and attenuate the activation of eosinophils.-
dc.languageeng-
dc.relation.ispartofMolecules-
dc.subjectAllergic inflammation-
dc.subjectAtopic dermatitis-
dc.subjectBerberine-
dc.subjectGallic acid-
dc.subjectPentaherbs formula-
dc.titleAnti-Inflammatory Activities of Pentaherbs Formula, Berberine, Gallic Acid and Chlorogenic Acid in Atopic Dermatitis-Like Skin Inflammation-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3390/molecules21040519-
dc.identifier.pmid27104513-
dc.identifier.scopuseid_2-s2.0-84968561608-
dc.identifier.volume21-
dc.identifier.issue4-
dc.identifier.spagearticle no. 519-
dc.identifier.epagearticle no. 519-
dc.identifier.eissn1420-3049-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats