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Article: Application of novel loop-mediated isothermal amplification (LAMP) for rapid authentication of the herbal tea ingredient Hedyotis diffusa Willd.

TitleApplication of novel loop-mediated isothermal amplification (LAMP) for rapid authentication of the herbal tea ingredient Hedyotis diffusa Willd.
Authors
Keywords(LAMP)
Baihuasheshecao
Hedyotis corymbosa
Hedyotis diffusa
Internal transcribed spacer (ITS)
Loop-mediated isothermal amplification
Molecular authentication
Issue Date2013
Citation
Food Chemistry, 2013, v. 141, n. 3, p. 2522-2525 How to Cite?
AbstractHedyotis diffusa Willd. (Baihuasheshecao) is an ingredient of herbal teas commonly consumed in the Orient and tropical Asia for cancer treatment and health maintenance. In the market, this ingredient is frequently adulterated by the related species Hedyotis corymbosa (L.) Lam. The objective of this study is to develop a novel loop-mediated isothermal amplification (LAMP) technique to differentiate H. diffusa from its adulterant H. corymbosa. A set of four internal control primers (F3, FIP, BIP and B3) were designed based on six loci in the internal transcribed spacer (ITS) for LAMP of both H. diffusa and H. corymbosa. Two specific primers (S-F3 and S-FIP) were designed for specific LAMP detection of H. diffusa only. Our data showed that LAMP was successful for both H. diffusa and H. corymbosa in internal control. In contrast, only H. diffusa was detected in specific LAMP using the specific primers S-F3 and S-FIP. This study showed that LAMP was useful to differentiate H. diffusa from its adulterant H. corymbosa. This study is significant for the verification of the authenticity for better quality control of this common herbal tea ingredient. The strategy of including an internal control assures the quality of the concerned DNA region for LAMP. © 2013 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/343121
ISSN
2023 Impact Factor: 8.5
2023 SCImago Journal Rankings: 1.745

 

DC FieldValueLanguage
dc.contributor.authorLi, Ming-
dc.contributor.authorWonga, Yuk Lau-
dc.contributor.authorJiang, Li Li-
dc.contributor.authorWonga, Ka Lok-
dc.contributor.authorWong, Yuen Ting-
dc.contributor.authorLau, Clara Bik San-
dc.contributor.authorShaw, Pang Chui-
dc.date.accessioned2024-05-10T09:05:36Z-
dc.date.available2024-05-10T09:05:36Z-
dc.date.issued2013-
dc.identifier.citationFood Chemistry, 2013, v. 141, n. 3, p. 2522-2525-
dc.identifier.issn0308-8146-
dc.identifier.urihttp://hdl.handle.net/10722/343121-
dc.description.abstractHedyotis diffusa Willd. (Baihuasheshecao) is an ingredient of herbal teas commonly consumed in the Orient and tropical Asia for cancer treatment and health maintenance. In the market, this ingredient is frequently adulterated by the related species Hedyotis corymbosa (L.) Lam. The objective of this study is to develop a novel loop-mediated isothermal amplification (LAMP) technique to differentiate H. diffusa from its adulterant H. corymbosa. A set of four internal control primers (F3, FIP, BIP and B3) were designed based on six loci in the internal transcribed spacer (ITS) for LAMP of both H. diffusa and H. corymbosa. Two specific primers (S-F3 and S-FIP) were designed for specific LAMP detection of H. diffusa only. Our data showed that LAMP was successful for both H. diffusa and H. corymbosa in internal control. In contrast, only H. diffusa was detected in specific LAMP using the specific primers S-F3 and S-FIP. This study showed that LAMP was useful to differentiate H. diffusa from its adulterant H. corymbosa. This study is significant for the verification of the authenticity for better quality control of this common herbal tea ingredient. The strategy of including an internal control assures the quality of the concerned DNA region for LAMP. © 2013 Elsevier Ltd. All rights reserved.-
dc.languageeng-
dc.relation.ispartofFood Chemistry-
dc.subject(LAMP)-
dc.subjectBaihuasheshecao-
dc.subjectHedyotis corymbosa-
dc.subjectHedyotis diffusa-
dc.subjectInternal transcribed spacer (ITS)-
dc.subjectLoop-mediated isothermal amplification-
dc.subjectMolecular authentication-
dc.titleApplication of novel loop-mediated isothermal amplification (LAMP) for rapid authentication of the herbal tea ingredient Hedyotis diffusa Willd.-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.foodchem.2013.05.085-
dc.identifier.pmid23870990-
dc.identifier.scopuseid_2-s2.0-84879228360-
dc.identifier.volume141-
dc.identifier.issue3-
dc.identifier.spage2522-
dc.identifier.epage2525-
dc.identifier.eissn1873-7072-

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