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- Publisher Website: 10.1016/j.intimp.2004.07.021
- Scopus: eid_2-s2.0-4444302510
- PMID: 15351324
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Article: Differential effect of Coriolus versicolor (Yunzhi) extract on cytokine production by murine lymphocytes in vitro
Title | Differential effect of Coriolus versicolor (Yunzhi) extract on cytokine production by murine lymphocytes in vitro |
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Authors | |
Keywords | Coriolus versicolor Immunomodulatory Interleukin-12 Interleukin-2 Lymphocytes T helper cytokines |
Issue Date | 2004 |
Citation | International Immunopharmacology, 2004, v. 4, n. 12, p. 1549-1557 How to Cite? |
Abstract | Being one of the commonly used Chinese medicinal herbs, Coriolus versicolor (CV), also named as Yunzhi, was known to possess both anti-tumor and immunopotentiating activities. The present study aimed to investigate the in vitro immunomodulatory effect of a standardized ethanola-water extract prepared from CV on the proliferation of murine splenic lymphocytes using the MTT assay, and the production of six T helper (Th)-related cytokines using the enzyme-linked immunosorbent assay (ELISA) technique. The results showed that the CV extract significantly augmented the proliferation of murine splenic lymphocytes in a time- and dose-dependent manner, maximally by 2.4-fold. Moreover, the production of two Th1-related cytokines, including interleukin (IL)-2 and IL-12, in culture supernatants from the CV extract-activated lymphocytes was prominently upregulated at 48 and 72 h. Positive correlations were found between the levels of these two cytokines and the MTT-based proliferative response. In contrast, the production of two other Th1-related cytokines, including interferon (IFN)-γ and IL-18, was significantly augmented only at 24 h, but not at 48 and 72 h. On the other hand, the levels of two Th2-related cytokines such as IL-4 and IL-6 were undetectable in the culture supernatants of lymphocytes treated with the CV extract. The CV extract was suggested to be a lymphocyte mitogen by differentially enhancing the production of Th1-related cytokines. © 2004 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/343032 |
ISSN | 2022 Impact Factor: 5.6 2020 SCImago Journal Rankings: 1.152 |
DC Field | Value | Language |
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dc.contributor.author | Ho, C. Y. | - |
dc.contributor.author | Lau, Clara B.S. | - |
dc.contributor.author | Kim, C. F. | - |
dc.contributor.author | Leung, K. N. | - |
dc.contributor.author | Fung, K. P. | - |
dc.contributor.author | Tse, T. F. | - |
dc.contributor.author | Chan, Helen H.L. | - |
dc.contributor.author | Chow, Moses S.S. | - |
dc.date.accessioned | 2024-05-10T09:04:55Z | - |
dc.date.available | 2024-05-10T09:04:55Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | International Immunopharmacology, 2004, v. 4, n. 12, p. 1549-1557 | - |
dc.identifier.issn | 1567-5769 | - |
dc.identifier.uri | http://hdl.handle.net/10722/343032 | - |
dc.description.abstract | Being one of the commonly used Chinese medicinal herbs, Coriolus versicolor (CV), also named as Yunzhi, was known to possess both anti-tumor and immunopotentiating activities. The present study aimed to investigate the in vitro immunomodulatory effect of a standardized ethanola-water extract prepared from CV on the proliferation of murine splenic lymphocytes using the MTT assay, and the production of six T helper (Th)-related cytokines using the enzyme-linked immunosorbent assay (ELISA) technique. The results showed that the CV extract significantly augmented the proliferation of murine splenic lymphocytes in a time- and dose-dependent manner, maximally by 2.4-fold. Moreover, the production of two Th1-related cytokines, including interleukin (IL)-2 and IL-12, in culture supernatants from the CV extract-activated lymphocytes was prominently upregulated at 48 and 72 h. Positive correlations were found between the levels of these two cytokines and the MTT-based proliferative response. In contrast, the production of two other Th1-related cytokines, including interferon (IFN)-γ and IL-18, was significantly augmented only at 24 h, but not at 48 and 72 h. On the other hand, the levels of two Th2-related cytokines such as IL-4 and IL-6 were undetectable in the culture supernatants of lymphocytes treated with the CV extract. The CV extract was suggested to be a lymphocyte mitogen by differentially enhancing the production of Th1-related cytokines. © 2004 Elsevier B.V. All rights reserved. | - |
dc.language | eng | - |
dc.relation.ispartof | International Immunopharmacology | - |
dc.subject | Coriolus versicolor | - |
dc.subject | Immunomodulatory | - |
dc.subject | Interleukin-12 | - |
dc.subject | Interleukin-2 | - |
dc.subject | Lymphocytes | - |
dc.subject | T helper cytokines | - |
dc.title | Differential effect of Coriolus versicolor (Yunzhi) extract on cytokine production by murine lymphocytes in vitro | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.intimp.2004.07.021 | - |
dc.identifier.pmid | 15351324 | - |
dc.identifier.scopus | eid_2-s2.0-4444302510 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1549 | - |
dc.identifier.epage | 1557 | - |