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Article: Metabolic and Gut Microbial Characterization of Obesity-Prone Mice under a High-Fat Diet

TitleMetabolic and Gut Microbial Characterization of Obesity-Prone Mice under a High-Fat Diet
Authors
Keywords16S rDNA
gut microbiota
metabolic profiling
obesity-prone
obesity-resistant
Issue Date2019
Citation
Journal of Proteome Research, 2019, v. 18, n. 4, p. 1703-1714 How to Cite?
AbstractObesity is characterized with high heterogeneity due to genetic abnormality, energy imbalance, gut dysbiosis, or a combination of all three. Obesity-prone (OP) and -resistant (OR) phenotypes are frequently observed in rodents, even in those given a high-fat diet (HFD). However, the underlying mechanisms are largely unknown. Male C57BL/6J mice were fed with chow or a HFD for 8 weeks. OP and OR mice were defined based on body weight gain, and integrated serum metabolic and gut microbial profiling was performed by the gas chromatography-mass spectroscopy-based metabolomic sequencing and pyrosequencing of 16S rDNA of cecum contents. A total of 60 differential metabolites were identified in comparisons among Con, OP, and OR groups, in which 27 were OP-related. These differential metabolites are mainly involved in glycolysis, lipids, and amino acids metabolism and the TCA cycle. Meanwhile, OP mice had a distinct profile in gut microbiota compared to those of OR or Con mice, which showed a reduced ratio of Firmicutes to Bacteroidetes and increased Proteobacteria. Moreover, the gut microbial alteration of OP mice was correlated with the changes of the key serum metabolites. OP-enriched Parasutterella from the Proteobacteria phylum correlated to most of metabolites, suggesting that it was essential in obesity. OP mice are distinct in metabolic and gut microbial profiles, and OP-related metabolites and bacteria are of significance for understanding obesity development.
Persistent Identifierhttp://hdl.handle.net/10722/342714
ISSN
2023 Impact Factor: 3.8
2023 SCImago Journal Rankings: 1.299
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGu, Yu-
dc.contributor.authorLiu, Can-
dc.contributor.authorZheng, Ningning-
dc.contributor.authorJia, Wei-
dc.contributor.authorZhang, Weidong-
dc.contributor.authorLi, Houkai-
dc.date.accessioned2024-04-17T07:05:44Z-
dc.date.available2024-04-17T07:05:44Z-
dc.date.issued2019-
dc.identifier.citationJournal of Proteome Research, 2019, v. 18, n. 4, p. 1703-1714-
dc.identifier.issn1535-3893-
dc.identifier.urihttp://hdl.handle.net/10722/342714-
dc.description.abstractObesity is characterized with high heterogeneity due to genetic abnormality, energy imbalance, gut dysbiosis, or a combination of all three. Obesity-prone (OP) and -resistant (OR) phenotypes are frequently observed in rodents, even in those given a high-fat diet (HFD). However, the underlying mechanisms are largely unknown. Male C57BL/6J mice were fed with chow or a HFD for 8 weeks. OP and OR mice were defined based on body weight gain, and integrated serum metabolic and gut microbial profiling was performed by the gas chromatography-mass spectroscopy-based metabolomic sequencing and pyrosequencing of 16S rDNA of cecum contents. A total of 60 differential metabolites were identified in comparisons among Con, OP, and OR groups, in which 27 were OP-related. These differential metabolites are mainly involved in glycolysis, lipids, and amino acids metabolism and the TCA cycle. Meanwhile, OP mice had a distinct profile in gut microbiota compared to those of OR or Con mice, which showed a reduced ratio of Firmicutes to Bacteroidetes and increased Proteobacteria. Moreover, the gut microbial alteration of OP mice was correlated with the changes of the key serum metabolites. OP-enriched Parasutterella from the Proteobacteria phylum correlated to most of metabolites, suggesting that it was essential in obesity. OP mice are distinct in metabolic and gut microbial profiles, and OP-related metabolites and bacteria are of significance for understanding obesity development.-
dc.languageeng-
dc.relation.ispartofJournal of Proteome Research-
dc.subject16S rDNA-
dc.subjectgut microbiota-
dc.subjectmetabolic profiling-
dc.subjectobesity-prone-
dc.subjectobesity-resistant-
dc.titleMetabolic and Gut Microbial Characterization of Obesity-Prone Mice under a High-Fat Diet-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.jproteome.8b00945-
dc.identifier.pmid30793608-
dc.identifier.scopuseid_2-s2.0-85063153961-
dc.identifier.volume18-
dc.identifier.issue4-
dc.identifier.spage1703-
dc.identifier.epage1714-
dc.identifier.eissn1535-3907-
dc.identifier.isiWOS:000464068900021-

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