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Article: A rapid UHPLC-QDa method for quantification of human salivary amino acid profiles

TitleA rapid UHPLC-QDa method for quantification of human salivary amino acid profiles
Authors
KeywordsAmino acids
Derivatization
Saliva
UHPLC-QDA detector
Issue Date2022
Citation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2022, v. 1211, article no. 123485 How to Cite?
AbstractAbnormal salivary amino acid (AA) levels may indicate dysfunction of the body. Being noninvasive, sampling easily and cost-effective of saliva, a rapid, precise and simple analysis method has become very important for quantitative salivary AA profiles. After one-step to precipitate protein, the resultant extraction was derived with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) within 10 min. Quantitation of AA profile was achieved within 6 min in a single run by ultra-high performance liquid chromatography coupled with a single quadrupole mass spectrometry (UHPLC-QDA detector). The method was validated with acceptable accuracy ranging from 80.33 % to 122.31 %, appropriate linearity with the coefficient (R2) more than 0.991, good intra- and inter-day precision, repeatability and stability (RSD < 15 %). The recoveries at three different spiked concentrations ranged over 79.18 %–125.36 % while the matrix effect was from −19.86 % to 11.95 %. This simple, rapid and robust method was successfully applied to quantify human salivary 30 amino acids, in which the levels of taurine, γ-aminobutyric acid, methionine and tryptophan in healthy people were close to the LOQs. Besides, the levels of histidine and cystine were not able to be measured due to their relatively high LOQs, which were considered as the limitations of this developed method.
Persistent Identifierhttp://hdl.handle.net/10722/342666
ISSN
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.539
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQu, Chun-
dc.contributor.authorJian, Chaohui-
dc.contributor.authorGe, Kun-
dc.contributor.authorZheng, Dan-
dc.contributor.authorBao, Yuqian-
dc.contributor.authorJia, Wei-
dc.contributor.authorZhao, Aihua-
dc.date.accessioned2024-04-17T07:05:24Z-
dc.date.available2024-04-17T07:05:24Z-
dc.date.issued2022-
dc.identifier.citationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2022, v. 1211, article no. 123485-
dc.identifier.issn1570-0232-
dc.identifier.urihttp://hdl.handle.net/10722/342666-
dc.description.abstractAbnormal salivary amino acid (AA) levels may indicate dysfunction of the body. Being noninvasive, sampling easily and cost-effective of saliva, a rapid, precise and simple analysis method has become very important for quantitative salivary AA profiles. After one-step to precipitate protein, the resultant extraction was derived with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) within 10 min. Quantitation of AA profile was achieved within 6 min in a single run by ultra-high performance liquid chromatography coupled with a single quadrupole mass spectrometry (UHPLC-QDA detector). The method was validated with acceptable accuracy ranging from 80.33 % to 122.31 %, appropriate linearity with the coefficient (R2) more than 0.991, good intra- and inter-day precision, repeatability and stability (RSD < 15 %). The recoveries at three different spiked concentrations ranged over 79.18 %–125.36 % while the matrix effect was from −19.86 % to 11.95 %. This simple, rapid and robust method was successfully applied to quantify human salivary 30 amino acids, in which the levels of taurine, γ-aminobutyric acid, methionine and tryptophan in healthy people were close to the LOQs. Besides, the levels of histidine and cystine were not able to be measured due to their relatively high LOQs, which were considered as the limitations of this developed method.-
dc.languageeng-
dc.relation.ispartofJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences-
dc.subjectAmino acids-
dc.subjectDerivatization-
dc.subjectSaliva-
dc.subjectUHPLC-QDA detector-
dc.titleA rapid UHPLC-QDa method for quantification of human salivary amino acid profiles-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jchromb.2022.123485-
dc.identifier.pmid36274351-
dc.identifier.scopuseid_2-s2.0-85140093910-
dc.identifier.volume1211-
dc.identifier.spagearticle no. 123485-
dc.identifier.epagearticle no. 123485-
dc.identifier.eissn1873-376X-
dc.identifier.isiWOS:000877646700001-

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