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- Publisher Website: 10.1007/s00216-017-0489-1
- Scopus: eid_2-s2.0-85021996932
- PMID: 28689325
- WOS: WOS:000408440300015
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Article: Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry in negative mode
Title | Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry in negative mode |
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Authors | |
Keywords | Bile acid Electrospray ionization High-performance liquid chromatography Ionization constant Mass spectrometry Structure-retention relationship |
Issue Date | 2017 |
Citation | Analytical and Bioanalytical Chemistry, 2017, v. 409, n. 23, p. 5533-5545 How to Cite? |
Abstract | Bile acids (BAs) are cholesterol metabolites with important biological functions. They undergo extensive host-gut microbial co-metabolisms during the enterohepatic circulation, creating a vast structural diversity and resulting in great challenges to separate and detect them. Based on the bioanalytical reports in the past decade, this work developed three chromatographic gradient methods to separate a total of 48 BA standards on an ethylene-bridged hybrid (BEH) C18 column and high-strength silica (HSS) T3 column and accordingly unraveled the factors affecting the separation and detection of them by liquid chromatography coupled with mass spectrometry (LC-MS). It was shown that both the acidity and ammonium levels in mobile phases reduced the electrospray ionization (ESI) of BAs as anions of [M−H]−, especially for those unconjugated ones without 12-hydroxylation. It was also found that the retention of taurine conjugates on the BEH C18 column was sensitive to the strength of formic acid and ammonium in mobile phases. By using the volatile buffers with an equivalent ammonium level as mobile phases, we comprehensively demonstrated the effects of the elution pH value on the retention behaviors of BAs on both the BEH C18 column and HSS T3 column. Based on the retention data acquired on a C18 column, we presented the ionization constants (pKa) of various BAs with the widest coverage beyond those of previous reports. When we made attempts to establish the structure-retention relationships (SRRs) of BAs, the lack of discriminative structural descriptors for BA stereoisomers emerged as the bottleneck problem. The methods and results presented in this work are especially useful for the development of reliable, sensitive, high-throughput, and robust LC-MS bioanalytical protocols for the quantitative metabolomic studies. [Figure not available: see fulltext.]. |
Persistent Identifier | http://hdl.handle.net/10722/342546 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.686 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Yin, Shanshan | - |
dc.contributor.author | Su, Mingming | - |
dc.contributor.author | Xie, Guoxiang | - |
dc.contributor.author | Li, Xuejing | - |
dc.contributor.author | Wei, Runmin | - |
dc.contributor.author | Liu, Changxiao | - |
dc.contributor.author | Lan, Ke | - |
dc.contributor.author | Jia, Wei | - |
dc.date.accessioned | 2024-04-17T07:04:35Z | - |
dc.date.available | 2024-04-17T07:04:35Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Analytical and Bioanalytical Chemistry, 2017, v. 409, n. 23, p. 5533-5545 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | http://hdl.handle.net/10722/342546 | - |
dc.description.abstract | Bile acids (BAs) are cholesterol metabolites with important biological functions. They undergo extensive host-gut microbial co-metabolisms during the enterohepatic circulation, creating a vast structural diversity and resulting in great challenges to separate and detect them. Based on the bioanalytical reports in the past decade, this work developed three chromatographic gradient methods to separate a total of 48 BA standards on an ethylene-bridged hybrid (BEH) C18 column and high-strength silica (HSS) T3 column and accordingly unraveled the factors affecting the separation and detection of them by liquid chromatography coupled with mass spectrometry (LC-MS). It was shown that both the acidity and ammonium levels in mobile phases reduced the electrospray ionization (ESI) of BAs as anions of [M−H]−, especially for those unconjugated ones without 12-hydroxylation. It was also found that the retention of taurine conjugates on the BEH C18 column was sensitive to the strength of formic acid and ammonium in mobile phases. By using the volatile buffers with an equivalent ammonium level as mobile phases, we comprehensively demonstrated the effects of the elution pH value on the retention behaviors of BAs on both the BEH C18 column and HSS T3 column. Based on the retention data acquired on a C18 column, we presented the ionization constants (pKa) of various BAs with the widest coverage beyond those of previous reports. When we made attempts to establish the structure-retention relationships (SRRs) of BAs, the lack of discriminative structural descriptors for BA stereoisomers emerged as the bottleneck problem. The methods and results presented in this work are especially useful for the development of reliable, sensitive, high-throughput, and robust LC-MS bioanalytical protocols for the quantitative metabolomic studies. [Figure not available: see fulltext.]. | - |
dc.language | eng | - |
dc.relation.ispartof | Analytical and Bioanalytical Chemistry | - |
dc.subject | Bile acid | - |
dc.subject | Electrospray ionization | - |
dc.subject | High-performance liquid chromatography | - |
dc.subject | Ionization constant | - |
dc.subject | Mass spectrometry | - |
dc.subject | Structure-retention relationship | - |
dc.title | Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry in negative mode | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00216-017-0489-1 | - |
dc.identifier.pmid | 28689325 | - |
dc.identifier.scopus | eid_2-s2.0-85021996932 | - |
dc.identifier.volume | 409 | - |
dc.identifier.issue | 23 | - |
dc.identifier.spage | 5533 | - |
dc.identifier.epage | 5545 | - |
dc.identifier.eissn | 1618-2650 | - |
dc.identifier.isi | WOS:000408440300015 | - |