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Article: Metabolic profiling reveals disorder of carbohydrate metabolism in mouse fibroblast cells induced by titanium dioxide nanoparticles

TitleMetabolic profiling reveals disorder of carbohydrate metabolism in mouse fibroblast cells induced by titanium dioxide nanoparticles
Authors
KeywordsCarbohydrate metabolism
Cytotoxicity
L929 cells
Metabolomic strategy
TiO nanoparticles 2
Issue Date2013
Citation
Journal of Applied Toxicology, 2013, v. 33, n. 12, p. 1442-1450 How to Cite?
AbstractAs titanium dioxide (TiO2) nanoparticles are widely used commercially, their potential biosafety and metabolic mechanism needs to be fully explained. In this study, the cytotoxicity of homogeneous and weakly aggregated (< 100nm) TiO2 nanoparticles was investigated by analyzing the changes in metabolite profiles both in mouse fibroblast (L929) cells and their corresponding culture media using gas chromatograph with a time-of-flight mass spectrometry (GC/TOFMS)-based metabolomic strategy. With multivariate statistics analysis, satisfactory separations were observed in principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models. Based on the variable importance in the OPLS-DA models, a series of differential metabolites were identified by comparison between TiO2 nanoparticle-treated L929 cells or their corresponding culture media and the control groups. It was found that the major biochemical metabolism (carbohydrate metabolism) was suppressed in TiO2 nanoparticle-treated L929 cells and their corresponding culture media. These results might account for the serious damage to energy metabolism in mitochondria and the increased cellular oxidation stress in TiO2 nanoparticle-induced L929 cells. These results also suggest that the metabolomic strategy had a great potential in evaluating the cytotoxicity of TiO2 nanoparticles and thus was very helpful in understanding its underlying molecular mechanisms. © 2012 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/342458
ISSN
2023 Impact Factor: 2.7
2023 SCImago Journal Rankings: 0.714
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJin, Chengyu-
dc.contributor.authorLiu, Yumin-
dc.contributor.authorSun, Limin-
dc.contributor.authorChen, Tianlu-
dc.contributor.authorZhang, Yinan-
dc.contributor.authorZhao, Aihua-
dc.contributor.authorWang, Xiaoyan-
dc.contributor.authorCristau, Melanie-
dc.contributor.authorWang, Kaisheng-
dc.contributor.authorJia, Wei-
dc.date.accessioned2024-04-17T07:03:58Z-
dc.date.available2024-04-17T07:03:58Z-
dc.date.issued2013-
dc.identifier.citationJournal of Applied Toxicology, 2013, v. 33, n. 12, p. 1442-1450-
dc.identifier.issn0260-437X-
dc.identifier.urihttp://hdl.handle.net/10722/342458-
dc.description.abstractAs titanium dioxide (TiO2) nanoparticles are widely used commercially, their potential biosafety and metabolic mechanism needs to be fully explained. In this study, the cytotoxicity of homogeneous and weakly aggregated (< 100nm) TiO2 nanoparticles was investigated by analyzing the changes in metabolite profiles both in mouse fibroblast (L929) cells and their corresponding culture media using gas chromatograph with a time-of-flight mass spectrometry (GC/TOFMS)-based metabolomic strategy. With multivariate statistics analysis, satisfactory separations were observed in principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models. Based on the variable importance in the OPLS-DA models, a series of differential metabolites were identified by comparison between TiO2 nanoparticle-treated L929 cells or their corresponding culture media and the control groups. It was found that the major biochemical metabolism (carbohydrate metabolism) was suppressed in TiO2 nanoparticle-treated L929 cells and their corresponding culture media. These results might account for the serious damage to energy metabolism in mitochondria and the increased cellular oxidation stress in TiO2 nanoparticle-induced L929 cells. These results also suggest that the metabolomic strategy had a great potential in evaluating the cytotoxicity of TiO2 nanoparticles and thus was very helpful in understanding its underlying molecular mechanisms. © 2012 John Wiley & Sons, Ltd.-
dc.languageeng-
dc.relation.ispartofJournal of Applied Toxicology-
dc.subjectCarbohydrate metabolism-
dc.subjectCytotoxicity-
dc.subjectL929 cells-
dc.subjectMetabolomic strategy-
dc.subjectTiO nanoparticles 2-
dc.titleMetabolic profiling reveals disorder of carbohydrate metabolism in mouse fibroblast cells induced by titanium dioxide nanoparticles-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jat.2808-
dc.identifier.pmid22996321-
dc.identifier.scopuseid_2-s2.0-84886096552-
dc.identifier.volume33-
dc.identifier.issue12-
dc.identifier.spage1442-
dc.identifier.epage1450-
dc.identifier.eissn1099-1263-
dc.identifier.isiWOS:000326277100008-

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