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Conference Paper: Use of the metabolomics approach to characterize chinese medicinal material Huangqi

TitleUse of the metabolomics approach to characterize chinese medicinal material Huangqi
Authors
Keywordsamplified fragment length polymorphisms
Astragalus membranaceus
gas chromatography time-of-flight mass spectrometry
metabolic fingerprinting
Issue Date2012
Citation
Molecular Plant, 2012, v. 5, n. 2, p. 376-386 How to Cite?
AbstractIntegration of the genetic and metabolic fingerprinting can provide a new approach to differentiate similar Traditional Chinese Medical (TCM) materials. Two leguminous plants, Mojia Huangqi and Menggu Huangqi, are important medical herbs and share great similarities in morphology, chemical constituent, and genomic DNA sequence. The taxonomy of Mojia Huangqi and Menggu Huangqi has been debated for more than 50years and discrimination of TCM materials directly affects the pharmacological and clinical effects. AFLP based genetic fingerprinting and GC-TOF/MS-based metabolic fingerprinting were used to successfully discriminate the two species. The results of AFLP supported the opinion that Menggu Huangqi was a variant of Mojia Huangqi. The metabolic fingerprinting showed growth locations have greater impacts on the metabolite composition and quantity than the genotypes (cultivated versus wild) in Menggu Huangqi. The difference of some soluble sugars, fatty acids, proline, and polyamine reflected plant adaptation to different growth environments. Using multivariate and univariate statistical analysis, three AFLP markers and eight metabolites were identified as candidate DNA and metabolic markers to distinguish the two herb materials. The correlation network between AFLP markers and metabolites revealed a complex correlation network, which indicated the special metabolic pathways and the regulation networks of Huangqi. © 2011 The Author Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.
Persistent Identifierhttp://hdl.handle.net/10722/342410
ISSN
2023 Impact Factor: 17.1
2023 SCImago Journal Rankings: 5.967
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDuan, Li Xin-
dc.contributor.authorChen, Tian Lu-
dc.contributor.authorLi, Ming-
dc.contributor.authorChen, Min-
dc.contributor.authorZhou, Ye Qing-
dc.contributor.authorCui, Guang Hong-
dc.contributor.authorZhao, Ai Hua-
dc.contributor.authorJia, Wei-
dc.contributor.authorHuang, Lu Qi-
dc.contributor.authorQi, Xiaoquan-
dc.date.accessioned2024-04-17T07:03:37Z-
dc.date.available2024-04-17T07:03:37Z-
dc.date.issued2012-
dc.identifier.citationMolecular Plant, 2012, v. 5, n. 2, p. 376-386-
dc.identifier.issn1674-2052-
dc.identifier.urihttp://hdl.handle.net/10722/342410-
dc.description.abstractIntegration of the genetic and metabolic fingerprinting can provide a new approach to differentiate similar Traditional Chinese Medical (TCM) materials. Two leguminous plants, Mojia Huangqi and Menggu Huangqi, are important medical herbs and share great similarities in morphology, chemical constituent, and genomic DNA sequence. The taxonomy of Mojia Huangqi and Menggu Huangqi has been debated for more than 50years and discrimination of TCM materials directly affects the pharmacological and clinical effects. AFLP based genetic fingerprinting and GC-TOF/MS-based metabolic fingerprinting were used to successfully discriminate the two species. The results of AFLP supported the opinion that Menggu Huangqi was a variant of Mojia Huangqi. The metabolic fingerprinting showed growth locations have greater impacts on the metabolite composition and quantity than the genotypes (cultivated versus wild) in Menggu Huangqi. The difference of some soluble sugars, fatty acids, proline, and polyamine reflected plant adaptation to different growth environments. Using multivariate and univariate statistical analysis, three AFLP markers and eight metabolites were identified as candidate DNA and metabolic markers to distinguish the two herb materials. The correlation network between AFLP markers and metabolites revealed a complex correlation network, which indicated the special metabolic pathways and the regulation networks of Huangqi. © 2011 The Author Published by the Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPB and IPPE, SIBS, CAS.-
dc.languageeng-
dc.relation.ispartofMolecular Plant-
dc.subjectamplified fragment length polymorphisms-
dc.subjectAstragalus membranaceus-
dc.subjectgas chromatography time-of-flight mass spectrometry-
dc.subjectmetabolic fingerprinting-
dc.titleUse of the metabolomics approach to characterize chinese medicinal material Huangqi-
dc.typeConference_Paper-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/mp/ssr093-
dc.identifier.scopuseid_2-s2.0-84859138219-
dc.identifier.volume5-
dc.identifier.issue2-
dc.identifier.spage376-
dc.identifier.epage386-
dc.identifier.eissn1752-9867-
dc.identifier.isiWOS:000301986700010-

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