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- Publisher Website: 10.3945/ajcn.2010.29807
- Scopus: eid_2-s2.0-78651290317
- PMID: 20943792
- WOS: WOS:000284993600031
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Article: Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and insulin resistance in primary human adipocytes
Title | Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and insulin resistance in primary human adipocytes |
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Authors | |
Issue Date | 2010 |
Citation | American Journal of Clinical Nutrition, 2010, v. 92, n. 6, p. 1511-1521 How to Cite? |
Abstract | Background: Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols reported to reduce inflammation or insulin resistance associated with obesity. Recently, we showed that grape powder extract, which contains quercetin and trans-RSV, attenuates markers of inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes. However, we do not know how quercetin and trans-RSV individually affected these outcomes. Objective: The aim of this study was to examine the extent to which quercetin and trans-RSV prevented inflammation or insulin resistance in primary cultures of human adipocytes treated with tumor necrosis factor-α (TNF-α) - an inflammatory cytokine elevated in the plasma and adipose tissue of obese, diabetic individuals. Design: Cultures of human adipocytes were pretreated with quercetin and trans-RSV followed by treatment with TNF-α. Subsequently, gene and protein markers of inflammation and insulin resistance were measured. Results: Quercetin, and to a lesser extent trans-RSV, attenuated the TNF-α-induced expression of inflammatory genes such as interleukin (IL)-6, IL-1β, IL-8, and monocyte chemoattractant protein-1 (MCP-1) and the secretion of IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-α-mediated phosphorylation of extracellular signal-related kinase and c-Jun-NH2 terminal kinase, whereas trans-RSV attenuated only c-Jun-NH2 terminal kinase phosphorylation. Quercetin and trans-RSV attenuated TNF-α-mediated phosphorylation of c-Jun and degradation of inhibitory κB protein. Quercetin, but not trans-RSV, decreased TNF-α-induced nuclear factor-κB transcriptional activity. Quercetin and trans-RSV attenuated the TNF-α-mediated suppression of peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ target genes and of PPARγ protein concentrations and transcriptional activity. Quercetin prevented the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1 and protein tyrosine phosphatase-1B gene expression and the suppression of insulin-stimulated glucose uptake, whereas trans-RSV prevented only the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1. Conclusion: These data suggest that quercetin is equally or more effective than trans-RSV in attenuating TNF-α-mediated inflammation and insulin resistance in primary human adipocytes. © 2010 American Society for Nutrition. |
Persistent Identifier | http://hdl.handle.net/10722/342386 |
ISSN | 2023 Impact Factor: 6.5 2023 SCImago Journal Rankings: 1.883 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chuang, Chia Chi | - |
dc.contributor.author | Martinez, Kristina | - |
dc.contributor.author | Xie, Guoxiang | - |
dc.contributor.author | Kennedy, Arion | - |
dc.contributor.author | Bumrungpert, Akkarach | - |
dc.contributor.author | Overman, Angel | - |
dc.contributor.author | Jia, Wei | - |
dc.contributor.author | McIntosh, Michael K. | - |
dc.date.accessioned | 2024-04-17T07:03:27Z | - |
dc.date.available | 2024-04-17T07:03:27Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | American Journal of Clinical Nutrition, 2010, v. 92, n. 6, p. 1511-1521 | - |
dc.identifier.issn | 0002-9165 | - |
dc.identifier.uri | http://hdl.handle.net/10722/342386 | - |
dc.description.abstract | Background: Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols reported to reduce inflammation or insulin resistance associated with obesity. Recently, we showed that grape powder extract, which contains quercetin and trans-RSV, attenuates markers of inflammation in human adipocytes and macrophages and insulin resistance in human adipocytes. However, we do not know how quercetin and trans-RSV individually affected these outcomes. Objective: The aim of this study was to examine the extent to which quercetin and trans-RSV prevented inflammation or insulin resistance in primary cultures of human adipocytes treated with tumor necrosis factor-α (TNF-α) - an inflammatory cytokine elevated in the plasma and adipose tissue of obese, diabetic individuals. Design: Cultures of human adipocytes were pretreated with quercetin and trans-RSV followed by treatment with TNF-α. Subsequently, gene and protein markers of inflammation and insulin resistance were measured. Results: Quercetin, and to a lesser extent trans-RSV, attenuated the TNF-α-induced expression of inflammatory genes such as interleukin (IL)-6, IL-1β, IL-8, and monocyte chemoattractant protein-1 (MCP-1) and the secretion of IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-α-mediated phosphorylation of extracellular signal-related kinase and c-Jun-NH2 terminal kinase, whereas trans-RSV attenuated only c-Jun-NH2 terminal kinase phosphorylation. Quercetin and trans-RSV attenuated TNF-α-mediated phosphorylation of c-Jun and degradation of inhibitory κB protein. Quercetin, but not trans-RSV, decreased TNF-α-induced nuclear factor-κB transcriptional activity. Quercetin and trans-RSV attenuated the TNF-α-mediated suppression of peroxisome proliferator-activated receptor γ (PPARγ) and PPARγ target genes and of PPARγ protein concentrations and transcriptional activity. Quercetin prevented the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1 and protein tyrosine phosphatase-1B gene expression and the suppression of insulin-stimulated glucose uptake, whereas trans-RSV prevented only the TNF-α-mediated serine phosphorylation of insulin receptor substrate-1. Conclusion: These data suggest that quercetin is equally or more effective than trans-RSV in attenuating TNF-α-mediated inflammation and insulin resistance in primary human adipocytes. © 2010 American Society for Nutrition. | - |
dc.language | eng | - |
dc.relation.ispartof | American Journal of Clinical Nutrition | - |
dc.title | Quercetin is equally or more effective than resveratrol in attenuating tumor necrosis factor-α-mediated inflammation and insulin resistance in primary human adipocytes | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3945/ajcn.2010.29807 | - |
dc.identifier.pmid | 20943792 | - |
dc.identifier.scopus | eid_2-s2.0-78651290317 | - |
dc.identifier.volume | 92 | - |
dc.identifier.issue | 6 | - |
dc.identifier.spage | 1511 | - |
dc.identifier.epage | 1521 | - |
dc.identifier.eissn | 1938-3207 | - |
dc.identifier.isi | WOS:000284993600031 | - |