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Article: Enrichment strategy of cysteine-containing peptides based on covalent chromatography

TitleEnrichment strategy of cysteine-containing peptides based on covalent chromatography
Authors
KeywordsCovalent chromatography
Cysteine-containing peptides
Enrichment
Liquid chromatography-tandem mass spectrometry
Proteome
Issue Date2010
Citation
Chinese Journal of Chromatography (Se Pu), 2010, v. 28, n. 2, p. 108-114 How to Cite?
AbstractAutomated multidimensional liquid chromatography-tandem mass spectrometry LC-MS/MS) is routinely applied in large scale proteome profiling. However global proteome analysis remains a technical challenge due to the issues associated with sample complexity by tryptic digestion. The application of tag containing peptide enrichment approach for sample pre-separation could reduce the complexity of protein digest. Here, we demonstrated a simple and highly efficient cysteine-containing peptide enrichment method using a thiol specific covalent resin. The cysteine-containing peptides from the tryptic digests of the complex protein mixtures were selected by covalent chromatography based on thiol-disulfide exchange, identified by mass spectrometry. The strategy was firstly optimized and evaluated by using the tryptic peptides of bovine serum albumin (BSA). Then the method was applied with a relatively complicated sample from a five standard protein mixture. The results of these studies show that the enrichment method of cysteine-containing peptides is highly specific, efficient and reproducible. The effectiveness of this method in reducing the sample complexity and improving the identification of peptides by mass spectrometry has enabled high-throughput, automatic and large-scale qualitative and quantitative proteome analyses.
Persistent Identifierhttp://hdl.handle.net/10722/342375
ISSN
2023 Impact Factor: 1.2
2023 SCImago Journal Rankings: 0.193

 

DC FieldValueLanguage
dc.contributor.authorMi, Wei-
dc.contributor.authorWang, Jing-
dc.contributor.authorYing, Wantao-
dc.contributor.authorJia, Wei-
dc.contributor.authorCai, Yun-
dc.contributor.authorQian, Xiaohong-
dc.date.accessioned2024-04-17T07:03:23Z-
dc.date.available2024-04-17T07:03:23Z-
dc.date.issued2010-
dc.identifier.citationChinese Journal of Chromatography (Se Pu), 2010, v. 28, n. 2, p. 108-114-
dc.identifier.issn1000-8713-
dc.identifier.urihttp://hdl.handle.net/10722/342375-
dc.description.abstractAutomated multidimensional liquid chromatography-tandem mass spectrometry LC-MS/MS) is routinely applied in large scale proteome profiling. However global proteome analysis remains a technical challenge due to the issues associated with sample complexity by tryptic digestion. The application of tag containing peptide enrichment approach for sample pre-separation could reduce the complexity of protein digest. Here, we demonstrated a simple and highly efficient cysteine-containing peptide enrichment method using a thiol specific covalent resin. The cysteine-containing peptides from the tryptic digests of the complex protein mixtures were selected by covalent chromatography based on thiol-disulfide exchange, identified by mass spectrometry. The strategy was firstly optimized and evaluated by using the tryptic peptides of bovine serum albumin (BSA). Then the method was applied with a relatively complicated sample from a five standard protein mixture. The results of these studies show that the enrichment method of cysteine-containing peptides is highly specific, efficient and reproducible. The effectiveness of this method in reducing the sample complexity and improving the identification of peptides by mass spectrometry has enabled high-throughput, automatic and large-scale qualitative and quantitative proteome analyses.-
dc.languageeng-
dc.relation.ispartofChinese Journal of Chromatography (Se Pu)-
dc.subjectCovalent chromatography-
dc.subjectCysteine-containing peptides-
dc.subjectEnrichment-
dc.subjectLiquid chromatography-tandem mass spectrometry-
dc.subjectProteome-
dc.titleEnrichment strategy of cysteine-containing peptides based on covalent chromatography-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3724/SP.J.1123.2012.00108-
dc.identifier.pmid20556946-
dc.identifier.scopuseid_2-s2.0-77954070542-
dc.identifier.volume28-
dc.identifier.issue2-
dc.identifier.spage108-
dc.identifier.epage114-

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