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Article: Determination of Endogenous Metabolites in Obesity Rat Urine by Pressurized Capillary Electrochromatography with Ethyl Chloroformate Derivatization

TitleDetermination of Endogenous Metabolites in Obesity Rat Urine by Pressurized Capillary Electrochromatography with Ethyl Chloroformate Derivatization
Authors
KeywordsEthyl chloroformate
Metabolite
Pressurized capillary electrochromatography (pCEC)
Urine
Issue Date2007
Citation
Chinese Journal of Analytical Chemistry, 2007, v. 35, n. 8, p. 1111-1115 How to Cite?
AbstractPressurized capillary electrochromatography (pCEC) method was developed to analyze metabolites in normal and obesity rat urine with ethyl chloroformate derivatization. The separation was performed on a capillary column (EP-150-30/50-5-C18), and good resolution was obtained with the mobile phase of water (0.01% TFA)-95% acetonitrile (0.01% TFA) using the following gradient: 0-30 min, 0-50 % B; 30-40 min, 50%-75% B. Under the optimized conditions, such as pressure of 13000 kPa, applied voltage of 2 kV, flow rate of 0.08 ml min-1, and room temperature, the contents of metabolites in the urine of normal and obesity rats were obtained. Under the optimum conditions, the correction coefficients were 0.9988-0.9999 in the range of 1.25-500 g l-1. The intra- and inter-day precisions were less than 5%. The average recoveries ranged from 95.9% to 103.2%. The method is simple, sensitive, and accurate for the determination of endogenous metabolites in rat urine sample. © 2007 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.
Persistent Identifierhttp://hdl.handle.net/10722/342316
ISSN
2023 Impact Factor: 1.2
2023 SCImago Journal Rankings: 0.355
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXie, Guo Xiang-
dc.contributor.authorQiu, Ming Feng-
dc.contributor.authorZhao, Ai Hua-
dc.contributor.authorPeng, Li-
dc.contributor.authorXue, Gu-
dc.contributor.authorWei, Jia-
dc.date.accessioned2024-04-17T07:02:55Z-
dc.date.available2024-04-17T07:02:55Z-
dc.date.issued2007-
dc.identifier.citationChinese Journal of Analytical Chemistry, 2007, v. 35, n. 8, p. 1111-1115-
dc.identifier.issn0253-3820-
dc.identifier.urihttp://hdl.handle.net/10722/342316-
dc.description.abstractPressurized capillary electrochromatography (pCEC) method was developed to analyze metabolites in normal and obesity rat urine with ethyl chloroformate derivatization. The separation was performed on a capillary column (EP-150-30/50-5-C18), and good resolution was obtained with the mobile phase of water (0.01% TFA)-95% acetonitrile (0.01% TFA) using the following gradient: 0-30 min, 0-50 % B; 30-40 min, 50%-75% B. Under the optimized conditions, such as pressure of 13000 kPa, applied voltage of 2 kV, flow rate of 0.08 ml min-1, and room temperature, the contents of metabolites in the urine of normal and obesity rats were obtained. Under the optimum conditions, the correction coefficients were 0.9988-0.9999 in the range of 1.25-500 g l-1. The intra- and inter-day precisions were less than 5%. The average recoveries ranged from 95.9% to 103.2%. The method is simple, sensitive, and accurate for the determination of endogenous metabolites in rat urine sample. © 2007 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.-
dc.languageeng-
dc.relation.ispartofChinese Journal of Analytical Chemistry-
dc.subjectEthyl chloroformate-
dc.subjectMetabolite-
dc.subjectPressurized capillary electrochromatography (pCEC)-
dc.subjectUrine-
dc.titleDetermination of Endogenous Metabolites in Obesity Rat Urine by Pressurized Capillary Electrochromatography with Ethyl Chloroformate Derivatization-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1872-2040(07)60073-5-
dc.identifier.scopuseid_2-s2.0-34848859839-
dc.identifier.volume35-
dc.identifier.issue8-
dc.identifier.spage1111-
dc.identifier.epage1115-
dc.identifier.eissn1872-2040-
dc.identifier.isiWOS:000250426600005-

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