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- Publisher Website: 10.1002/anie.201409791
- Scopus: eid_2-s2.0-84920815414
- PMID: 25418121
- WOS: WOS:000348372200056
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Article: Phase behavior of a designed cyclopropyl analogue of monoolein: Implications for low-temperature membrane protein crystallization
Title | Phase behavior of a designed cyclopropyl analogue of monoolein: Implications for low-temperature membrane protein crystallization |
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Authors | |
Keywords | Lipid synthesis Membrane proteins Mesophases Phase behavior Small-angle X-ray scattering |
Issue Date | 2015 |
Citation | Angewandte Chemie - International Edition, 2015, v. 54, n. 3, p. 1027-1031 How to Cite? |
Abstract | Lipidic cubic phases (LCPs) are used in areas ranging from membrane biology to biodevices. Because some membrane proteins are notoriously unstable at room temperature, and available LCPs undergo transformation to lamellar phases at low temperatures, development of stable low-temperature LCPs for biophysical studies of membrane proteins is called for. Monodihydrosterculin (MDS) is a designer lipid based on monoolein (MO) with a configurationally restricted cyclopropyl ring replacing the olefin. Small-angle X-ray scattering (SAXS) analyses revealed a phase diagram for MDS lacking the high-temperature, highly curved reverse hexagonal phase typical for MO, and extending the cubic phase boundary to lower temperature, thereby establishing the relationship between lipid molecular structure and mesophase behavior. The use of MDS as a new material for LCP-based membrane protein crystallization at low temperature was demonstrated by crystallizing bacteriorhodopsin at 20°C as well as 4°C. |
Persistent Identifier | http://hdl.handle.net/10722/341165 |
ISSN | 2023 Impact Factor: 16.1 2023 SCImago Journal Rankings: 5.300 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Salvati Manni, Livia | - |
dc.contributor.author | Zabara, Alexandru | - |
dc.contributor.author | Osornio, Yazmin M. | - |
dc.contributor.author | Schöppe, Jendrik | - |
dc.contributor.author | Batyuk, Alexander | - |
dc.contributor.author | Plückthun, Andreas | - |
dc.contributor.author | Siegel, Jay S. | - |
dc.contributor.author | Mezzenga, Raffaele | - |
dc.contributor.author | Landau, Ehud M. | - |
dc.date.accessioned | 2024-03-13T08:40:40Z | - |
dc.date.available | 2024-03-13T08:40:40Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Angewandte Chemie - International Edition, 2015, v. 54, n. 3, p. 1027-1031 | - |
dc.identifier.issn | 1433-7851 | - |
dc.identifier.uri | http://hdl.handle.net/10722/341165 | - |
dc.description.abstract | Lipidic cubic phases (LCPs) are used in areas ranging from membrane biology to biodevices. Because some membrane proteins are notoriously unstable at room temperature, and available LCPs undergo transformation to lamellar phases at low temperatures, development of stable low-temperature LCPs for biophysical studies of membrane proteins is called for. Monodihydrosterculin (MDS) is a designer lipid based on monoolein (MO) with a configurationally restricted cyclopropyl ring replacing the olefin. Small-angle X-ray scattering (SAXS) analyses revealed a phase diagram for MDS lacking the high-temperature, highly curved reverse hexagonal phase typical for MO, and extending the cubic phase boundary to lower temperature, thereby establishing the relationship between lipid molecular structure and mesophase behavior. The use of MDS as a new material for LCP-based membrane protein crystallization at low temperature was demonstrated by crystallizing bacteriorhodopsin at 20°C as well as 4°C. | - |
dc.language | eng | - |
dc.relation.ispartof | Angewandte Chemie - International Edition | - |
dc.subject | Lipid synthesis | - |
dc.subject | Membrane proteins | - |
dc.subject | Mesophases | - |
dc.subject | Phase behavior | - |
dc.subject | Small-angle X-ray scattering | - |
dc.title | Phase behavior of a designed cyclopropyl analogue of monoolein: Implications for low-temperature membrane protein crystallization | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/anie.201409791 | - |
dc.identifier.pmid | 25418121 | - |
dc.identifier.scopus | eid_2-s2.0-84920815414 | - |
dc.identifier.volume | 54 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 1027 | - |
dc.identifier.epage | 1031 | - |
dc.identifier.eissn | 1521-3773 | - |
dc.identifier.isi | WOS:000348372200056 | - |