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Article: Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

TitleUncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing
Authors
KeywordsNiche
Paraspinal muscle
Satellite cells
Senescence
Single-nucleus RNA sequencing
Issue Date1-Nov-2023
PublisherChongqing Medical University
Citation
Genes & diseases, 2023, v. 10, n. 6, p. 2597-2613 How to Cite?
AbstractTo uncover the role of satellite cells (SCs) in paravertebral muscle development and aging, we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo (day 16.5) to old (month 24) stages. Eight cell types, including SCs, fast muscle cells, and slow muscle cells, were identified. An energy metabolism-related gene set, TCA CYCLE IN SENESCENCE, was enriched in SCs. Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns. Among them, Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE; Pgam2, Sod1, and Suclg1 are transcription factors closely associated with skeletal muscle energy metabolism. Transcription factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs, which regulated Pdha1 expression and were associated with skeletal muscle development. These findings hint that energy metabolism may be pivotal in SCs development and aging. Three ligand-receptor pairs of extracellular matrix (ECM)-receptor interactions, Lamc1-Dag1, Lama2-Dag1, and Hspg2-Dag1, may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal. Finally, we built the first database of a skeletal muscle single-cell transcriptome, the Musculoskeletal Cell Atlas (http://www.mskca.tech), which lists 630,040 skeletal muscle cells and provides interactive visualization, a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging. Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.
Persistent Identifierhttp://hdl.handle.net/10722/338657
ISSN
2023 Impact Factor: 6.9
2023 SCImago Journal Rankings: 1.446
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQiu, X-
dc.contributor.authorWang, HY-
dc.contributor.authorYang, ZY-
dc.contributor.authorSun, LM-
dc.contributor.authorLiu, SN-
dc.contributor.authorFan, CQ-
dc.contributor.authorZhu, F-
dc.date.accessioned2024-03-11T10:30:32Z-
dc.date.available2024-03-11T10:30:32Z-
dc.date.issued2023-11-01-
dc.identifier.citationGenes & diseases, 2023, v. 10, n. 6, p. 2597-2613-
dc.identifier.issn2352-4820-
dc.identifier.urihttp://hdl.handle.net/10722/338657-
dc.description.abstractTo uncover the role of satellite cells (SCs) in paravertebral muscle development and aging, we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo (day 16.5) to old (month 24) stages. Eight cell types, including SCs, fast muscle cells, and slow muscle cells, were identified. An energy metabolism-related gene set, TCA CYCLE IN SENESCENCE, was enriched in SCs. Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns. Among them, Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE; Pgam2, Sod1, and Suclg1 are transcription factors closely associated with skeletal muscle energy metabolism. Transcription factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs, which regulated Pdha1 expression and were associated with skeletal muscle development. These findings hint that energy metabolism may be pivotal in SCs development and aging. Three ligand-receptor pairs of extracellular matrix (ECM)-receptor interactions, Lamc1-Dag1, Lama2-Dag1, and Hspg2-Dag1, may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal. Finally, we built the first database of a skeletal muscle single-cell transcriptome, the Musculoskeletal Cell Atlas (http://www.mskca.tech), which lists 630,040 skeletal muscle cells and provides interactive visualization, a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging. Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.-
dc.languageeng-
dc.publisherChongqing Medical University-
dc.relation.ispartofGenes & diseases-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectNiche-
dc.subjectParaspinal muscle-
dc.subjectSatellite cells-
dc.subjectSenescence-
dc.subjectSingle-nucleus RNA sequencing-
dc.titleUncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing-
dc.typeArticle-
dc.identifier.doi10.1016/j.gendis.2023.01.005-
dc.identifier.scopuseid_2-s2.0-85149460111-
dc.identifier.volume10-
dc.identifier.issue6-
dc.identifier.spage2597-
dc.identifier.epage2613-
dc.identifier.eissn2352-3042-
dc.identifier.isiWOS:001053096500001-
dc.identifier.issnl2352-3042-

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