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Article: A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation

TitleA key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation
Authors
KeywordsabfA cluster
B. longum
biomarker
fecal microbiota transplantation
functional constipation
gastrointestinal motility
gut microbiome
probiotics
short-chain fatty acids
strain-specific
Issue Date13-Dec-2023
PublisherCell Press
Citation
Cell Host & Microbe, 2023, v. 31, n. 12, p. 1989-2006 How to Cite?
Abstract

Impaired gastrointestinal motility is associated with gut dysbiosis. Probiotics such as Bifidobacteria can improve this bowel disorder, yet efficacy is strain-dependent. We determine that a genetic factor, the abfA cluster governing arabinan utilization, in Bifidobacterium longum impacts treatment efficacy against functional constipation (FC). In mice with FC, B. longum, but not an abfA mutant, improved gastrointestinal transit time, effects that were dependent upon dietary arabinan. abfA genes were identified in other commensal bacteria, whose effects in ameliorating murine FC were similarly abfA-dependent. In a double-blind, randomized, placebo-controlled clinical trial, supplementation with abfA-cluster-carrying B. longum, but not an abfA-deficient strain, enriched arabinan-utilization residents, increased beneficial metabolites, and improved FC symptoms. Across human cohorts, abfA-cluster abundance can predict FC and transplantation of abfA cluster-enriched human microbiota to FC-induced germ-free mice improved gut motility. Collectively, these findings demonstrate a role for microbial abfA cluster in ameliorating FC, establishing principles for genomics-directed probiotic therapies.


Persistent Identifierhttp://hdl.handle.net/10722/338424
ISSN
2023 Impact Factor: 20.6
2023 SCImago Journal Rankings: 7.760
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, CC-
dc.contributor.authorYu, LL-
dc.contributor.authorMa, CC-
dc.contributor.authorJiang, SM-
dc.contributor.authorZhang, YF-
dc.contributor.authorWang, SH-
dc.contributor.authorTian, FW-
dc.contributor.authorXue, YZ-
dc.contributor.authorZhao, JX-
dc.contributor.authorZhang, H-
dc.contributor.authorLiu, LM-
dc.contributor.authorChen, W-
dc.contributor.authorHuang, S-
dc.contributor.authorZhang, JC-
dc.contributor.authorZhai, QX-
dc.date.accessioned2024-03-11T10:28:45Z-
dc.date.available2024-03-11T10:28:45Z-
dc.date.issued2023-12-13-
dc.identifier.citationCell Host & Microbe, 2023, v. 31, n. 12, p. 1989-2006-
dc.identifier.issn1931-3128-
dc.identifier.urihttp://hdl.handle.net/10722/338424-
dc.description.abstract<p>Impaired gastrointestinal motility is associated with gut dysbiosis. Probiotics such as <em>Bifidobacteria</em> can improve this bowel disorder, yet efficacy is strain-dependent. We determine that a genetic factor, the <em>abfA</em> cluster governing arabinan utilization, in <em>Bifidobacterium longum</em> impacts treatment efficacy against functional constipation (FC). In mice with FC, <em>B. longum,</em> but not an <em>abfA</em> mutant, improved gastrointestinal transit time, effects that were dependent upon dietary arabinan. <em>abfA </em>genes were identified in other commensal bacteria, whose effects in ameliorating murine FC were similarly <em>abfA-</em>dependent. In a double-blind, randomized, placebo-controlled clinical trial, supplementation with <em>abfA</em>-cluster-carrying <em>B. longum,</em> but not an <em>abfA</em>-deficient strain, enriched arabinan-utilization residents, increased beneficial metabolites, and improved FC symptoms. Across human cohorts, <em>abfA</em>-cluster abundance can predict FC and transplantation of <em>abfA</em> cluster-enriched human microbiota to FC-induced germ-free mice improved gut motility. Collectively, these findings demonstrate a role for microbial <em>abfA</em> cluster in ameliorating FC, establishing principles for genomics-directed probiotic therapies.<br></p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofCell Host & Microbe-
dc.subjectabfA cluster-
dc.subjectB. longum-
dc.subjectbiomarker-
dc.subjectfecal microbiota transplantation-
dc.subjectfunctional constipation-
dc.subjectgastrointestinal motility-
dc.subjectgut microbiome-
dc.subjectprobiotics-
dc.subjectshort-chain fatty acids-
dc.subjectstrain-specific-
dc.titleA key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation-
dc.typeArticle-
dc.identifier.doi10.1016/j.chom.2023.10.011-
dc.identifier.scopuseid_2-s2.0-85179102164-
dc.identifier.volume31-
dc.identifier.issue12-
dc.identifier.spage1989-
dc.identifier.epage2006-
dc.identifier.eissn1934-6069-
dc.identifier.isiWOS:001139101900001-
dc.identifier.issnl1931-3128-

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