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- Publisher Website: 10.1155/2019/8672604
- Scopus: eid_2-s2.0-85073054991
- PMID: 31637266
- WOS: WOS:000488465900001
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Article: The Possible Role of Neutrophils in the Induction of Osteoclastogenesis
Title | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
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Authors | |
Issue Date | 2019 |
Citation | Journal of Immunology Research, 2019, v. 2019, article no. 8672604 How to Cite? |
Abstract | The ligand of the receptor activator of NF-κB (RANKL) is a key molecule in the formation of osteoclasts, the key cells that cause the disease-associated alveolar bone resorption in periodontitis. We hypothesized that polymorphonuclear leukocytes (PMNs), found as the most prominent cells of inflamed periodontal tissues, could play an important role in providing signals to trigger osteoclastogenesis and thus activating pathological bone resorption in periodontitis. RANKL expression was investigated on circulatory PMNs (cPMNs) and oral PMNs (oPMNs) taken from both controls and periodontitis patients. On average, 2.3% and 2.4% RANKL expression was detected on the cPMNs and oPMNs from periodontitis patients, which did not differ significantly from healthy controls. Since cPMNs may acquire a more osteoclastogenesis-facilitating phenotype while migrating into the inflamed periodontium, we next investigated whether stimulated (with LPS, TNF-α, or IL-6) cPMNs have the capacity to contribute to osteoclastogenesis. Enduring surface expression of RANKL for short-lived cells as cPMNs was achieved by fixating stimulated cPMNs. RANKL expression on stimulated cPMNs, as assessed by flow cytometry and immunohistochemistry, was limited (6.48±0.72%, mean expression±SEM) after 24 and 48 hours of stimulation with LPS. Likewise, stimulation with TNF-α and IL-6 resulted in limited RANKL expression levels. These limited levels of expression did not induce osteoclastogenesis when cocultured with preosteoclasts for 10 days. We report that, under the aforementioned experimental conditions, neither cPMNs nor oPMNs directly induced osteoclastogenesis. Further elucidation of the key cellular players and immune mediators that stimulate alveolar bone resorption in periodontitis will help to unravel its pathogenesis. |
Persistent Identifier | http://hdl.handle.net/10722/336767 |
ISSN | 2023 Impact Factor: 3.5 2023 SCImago Journal Rankings: 0.944 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Moonen, Carolyn G.J. | - |
dc.contributor.author | De Vries, Teun J. | - |
dc.contributor.author | Rijkschroeff, Patrick | - |
dc.contributor.author | Poubelle, Patrice E. | - |
dc.contributor.author | Nicu, Elena A. | - |
dc.contributor.author | Loos, Bruno G. | - |
dc.date.accessioned | 2024-02-29T06:56:24Z | - |
dc.date.available | 2024-02-29T06:56:24Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Journal of Immunology Research, 2019, v. 2019, article no. 8672604 | - |
dc.identifier.issn | 2314-8861 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336767 | - |
dc.description.abstract | The ligand of the receptor activator of NF-κB (RANKL) is a key molecule in the formation of osteoclasts, the key cells that cause the disease-associated alveolar bone resorption in periodontitis. We hypothesized that polymorphonuclear leukocytes (PMNs), found as the most prominent cells of inflamed periodontal tissues, could play an important role in providing signals to trigger osteoclastogenesis and thus activating pathological bone resorption in periodontitis. RANKL expression was investigated on circulatory PMNs (cPMNs) and oral PMNs (oPMNs) taken from both controls and periodontitis patients. On average, 2.3% and 2.4% RANKL expression was detected on the cPMNs and oPMNs from periodontitis patients, which did not differ significantly from healthy controls. Since cPMNs may acquire a more osteoclastogenesis-facilitating phenotype while migrating into the inflamed periodontium, we next investigated whether stimulated (with LPS, TNF-α, or IL-6) cPMNs have the capacity to contribute to osteoclastogenesis. Enduring surface expression of RANKL for short-lived cells as cPMNs was achieved by fixating stimulated cPMNs. RANKL expression on stimulated cPMNs, as assessed by flow cytometry and immunohistochemistry, was limited (6.48±0.72%, mean expression±SEM) after 24 and 48 hours of stimulation with LPS. Likewise, stimulation with TNF-α and IL-6 resulted in limited RANKL expression levels. These limited levels of expression did not induce osteoclastogenesis when cocultured with preosteoclasts for 10 days. We report that, under the aforementioned experimental conditions, neither cPMNs nor oPMNs directly induced osteoclastogenesis. Further elucidation of the key cellular players and immune mediators that stimulate alveolar bone resorption in periodontitis will help to unravel its pathogenesis. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Immunology Research | - |
dc.title | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1155/2019/8672604 | - |
dc.identifier.pmid | 31637266 | - |
dc.identifier.scopus | eid_2-s2.0-85073054991 | - |
dc.identifier.volume | 2019 | - |
dc.identifier.spage | article no. 8672604 | - |
dc.identifier.epage | article no. 8672604 | - |
dc.identifier.eissn | 2314-7156 | - |
dc.identifier.isi | WOS:000488465900001 | - |