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- Publisher Website: 10.1016/j.dental.2020.05.012
- Scopus: eid_2-s2.0-85086510707
- PMID: 32563521
- WOS: WOS:000560041300012
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Article: Antibacterial and mechanical properties of arginine-containing glass ionomer cements
Title | Antibacterial and mechanical properties of arginine-containing glass ionomer cements |
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Authors | |
Keywords | Arginine Caries Glass ionomer cements Prevention |
Issue Date | 2020 |
Citation | Dental Materials, 2020, v. 36, n. 9, p. 1226-1240 How to Cite? |
Abstract | Objective: The study investigated the effect of incorporating L-arginine (Arg) in a glass ionomer cement (GIC) on its mechanical properties and antibacterial potential. Methods: Pre-determined proportions (1%, 2%, and 4% by wt.) of Arg were incorporated in GIC powder; while GIC without Arg served as control. The flexural strength, nanohardness, surface roughness, elemental analysis using SEM-EDX (n = 6) and F/Arg/Ca/Al/Si release in deionized water for 21 days were assessed. The antibacterial potential was evaluated in a multi-species biofilm model with Streptococcus mutans, Streptococcus sanguinis, Streptococcus gordonii, and Lactobacillus acidophilus for 72 h. Real-time qPCR was used to analyse biofilm bacterial concentrations. Propidium monoazide modification of real-time qPCR was performed to quantify viable/dead bacteria. The pH, lactic acid, ADS activity, and H2O2 metabolism were measured. Confocal microscopy was used to investigate the biofilm bacterial live/dead cells, density, and thickness. Results: There was no difference in flexural strength among the different groups (p > 0.05). No significant difference in nanohardness and surface roughness was observed between 4% Arg + GIC and control (p > 0.05). The 4% Arg + GIC showed significantly higher F/Arg/Al/Si release than the other groups (p < 0.05), reduced total bacterial concentration and growth inhibition of viable S. mutans and S. sanguinis (p < 0.05). Lactic acid formation for 4% Arg + GIC was significantly higher than 1% Arg + GIC (p < 0.05). The spent media pH of 4% Arg + GIC was higher than the other groups (p < 0.05), with proportionately lower ammonia and higher H2O2 released (p < 0.05). Significance: Addition of 4% L-arginine in GIC enhanced its antibacterial activity via a biofilm modulatory effect for microbial homeostasis, with no detrimental effect on its mechanical properties. |
Persistent Identifier | http://hdl.handle.net/10722/336237 |
ISSN | 2023 Impact Factor: 4.6 2023 SCImago Journal Rankings: 1.186 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bijle, Mohammed Nadeem | - |
dc.contributor.author | Ekambaram, Manikandan | - |
dc.contributor.author | Lo, Edward C.M. | - |
dc.contributor.author | Yiu, Cynthia Kar Yung | - |
dc.date.accessioned | 2024-01-15T08:24:45Z | - |
dc.date.available | 2024-01-15T08:24:45Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Dental Materials, 2020, v. 36, n. 9, p. 1226-1240 | - |
dc.identifier.issn | 0109-5641 | - |
dc.identifier.uri | http://hdl.handle.net/10722/336237 | - |
dc.description.abstract | Objective: The study investigated the effect of incorporating L-arginine (Arg) in a glass ionomer cement (GIC) on its mechanical properties and antibacterial potential. Methods: Pre-determined proportions (1%, 2%, and 4% by wt.) of Arg were incorporated in GIC powder; while GIC without Arg served as control. The flexural strength, nanohardness, surface roughness, elemental analysis using SEM-EDX (n = 6) and F/Arg/Ca/Al/Si release in deionized water for 21 days were assessed. The antibacterial potential was evaluated in a multi-species biofilm model with Streptococcus mutans, Streptococcus sanguinis, Streptococcus gordonii, and Lactobacillus acidophilus for 72 h. Real-time qPCR was used to analyse biofilm bacterial concentrations. Propidium monoazide modification of real-time qPCR was performed to quantify viable/dead bacteria. The pH, lactic acid, ADS activity, and H2O2 metabolism were measured. Confocal microscopy was used to investigate the biofilm bacterial live/dead cells, density, and thickness. Results: There was no difference in flexural strength among the different groups (p > 0.05). No significant difference in nanohardness and surface roughness was observed between 4% Arg + GIC and control (p > 0.05). The 4% Arg + GIC showed significantly higher F/Arg/Al/Si release than the other groups (p < 0.05), reduced total bacterial concentration and growth inhibition of viable S. mutans and S. sanguinis (p < 0.05). Lactic acid formation for 4% Arg + GIC was significantly higher than 1% Arg + GIC (p < 0.05). The spent media pH of 4% Arg + GIC was higher than the other groups (p < 0.05), with proportionately lower ammonia and higher H2O2 released (p < 0.05). Significance: Addition of 4% L-arginine in GIC enhanced its antibacterial activity via a biofilm modulatory effect for microbial homeostasis, with no detrimental effect on its mechanical properties. | - |
dc.language | eng | - |
dc.relation.ispartof | Dental Materials | - |
dc.subject | Arginine | - |
dc.subject | Caries | - |
dc.subject | Glass ionomer cements | - |
dc.subject | Prevention | - |
dc.title | Antibacterial and mechanical properties of arginine-containing glass ionomer cements | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.dental.2020.05.012 | - |
dc.identifier.pmid | 32563521 | - |
dc.identifier.scopus | eid_2-s2.0-85086510707 | - |
dc.identifier.volume | 36 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1226 | - |
dc.identifier.epage | 1240 | - |
dc.identifier.isi | WOS:000560041300012 | - |