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- Publisher Website: 10.3390/jcm9092920
- Scopus: eid_2-s2.0-85094850424
- WOS: WOS:000579919900001
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Article: A pilot study on microrna profile in tear fluid to predict response to anti-vegf treatments for diabetic macular edema
Title | A pilot study on microrna profile in tear fluid to predict response to anti-vegf treatments for diabetic macular edema |
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Authors | |
Keywords | Aflibercept Anti-vascular endothelial growth factor Bevacizumab Biomarker Diabetic macular edema MicroRNA |
Issue Date | 2020 |
Citation | Journal of Clinical Medicine, 2020, v. 9, n. 9, p. 1-18 How to Cite? |
Abstract | (1) Background: Intravitreal anti-vascular endothelial growth factor (anti-VEGF) is an established treatment for center-involving diabetic macular edema (ci-DME). However, the clinical response is heterogeneous. This study investigated miRNAs as a biomarker to predict treatment response to anti-VEGF in DME. (2) Methods: Tear fluid, aqueous, and blood were collected from patients with treatment-naïve DME for miRNA expression profiling with quantitative polymerase chain reaction. Differentially expressed miRNAs between good and poor responders were identified from tear fluid. Bioinformatics analysis with the miEAA tool, miRTarBase Annotations, Gene Ontology categories, KEGG, and miRWalk pathways identified interactions between enriched miRNAs and biological pathways. (3) Results: Of 24 participants, 28 eyes received bevacizumab (15 eyes) or aflibercept (13 eyes). Tear fluid had the most detectable miRNA species (N = 315), followed by serum (N = 309), then aqueous humor (N = 134). MiRNAs that correlated with change in macular thickness were miR-214-3p, miR-320d, and hsa-miR-874-3p in good responders; and miR-98-5p, miR-196b-5p, and miR-454-3p in poor responders. VEGF-related pathways and the angiogenin-PRI complex were enriched in good responders, while transforming growth factor-β and insulin-like growth factor pathways were enriched in poor responders. (4) Conclusions: We reported a panel of novel miRNAs that provide insight into biological pathways in DME. Validation in larger independent cohorts is needed to determine the predictive performance of these miRNA candidate biomarkers. |
Persistent Identifier | http://hdl.handle.net/10722/335855 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, Hwei Wuen | - |
dc.contributor.author | Yang, Binxia | - |
dc.contributor.author | Wong, Wendy | - |
dc.contributor.author | Blakeley, Paul | - |
dc.contributor.author | Seah, Ivan | - |
dc.contributor.author | Tan, Queenie Shu Woon | - |
dc.contributor.author | Wang, Haofei | - |
dc.contributor.author | Bhargava, Mayuri | - |
dc.contributor.author | Lin, Hazel Anne | - |
dc.contributor.author | Chai, Charmaine H.C. | - |
dc.contributor.author | Mangunkusumo, Erlangga Ariadarma | - |
dc.contributor.author | Thet, Naing | - |
dc.contributor.author | Yuen, Yew Sen | - |
dc.contributor.author | Sethi, Raman | - |
dc.contributor.author | Wang, Si | - |
dc.contributor.author | Hunziker, Walter | - |
dc.contributor.author | Lingam, Gopal | - |
dc.contributor.author | Su, Xinyi | - |
dc.date.accessioned | 2023-12-28T08:49:15Z | - |
dc.date.available | 2023-12-28T08:49:15Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Clinical Medicine, 2020, v. 9, n. 9, p. 1-18 | - |
dc.identifier.uri | http://hdl.handle.net/10722/335855 | - |
dc.description.abstract | (1) Background: Intravitreal anti-vascular endothelial growth factor (anti-VEGF) is an established treatment for center-involving diabetic macular edema (ci-DME). However, the clinical response is heterogeneous. This study investigated miRNAs as a biomarker to predict treatment response to anti-VEGF in DME. (2) Methods: Tear fluid, aqueous, and blood were collected from patients with treatment-naïve DME for miRNA expression profiling with quantitative polymerase chain reaction. Differentially expressed miRNAs between good and poor responders were identified from tear fluid. Bioinformatics analysis with the miEAA tool, miRTarBase Annotations, Gene Ontology categories, KEGG, and miRWalk pathways identified interactions between enriched miRNAs and biological pathways. (3) Results: Of 24 participants, 28 eyes received bevacizumab (15 eyes) or aflibercept (13 eyes). Tear fluid had the most detectable miRNA species (N = 315), followed by serum (N = 309), then aqueous humor (N = 134). MiRNAs that correlated with change in macular thickness were miR-214-3p, miR-320d, and hsa-miR-874-3p in good responders; and miR-98-5p, miR-196b-5p, and miR-454-3p in poor responders. VEGF-related pathways and the angiogenin-PRI complex were enriched in good responders, while transforming growth factor-β and insulin-like growth factor pathways were enriched in poor responders. (4) Conclusions: We reported a panel of novel miRNAs that provide insight into biological pathways in DME. Validation in larger independent cohorts is needed to determine the predictive performance of these miRNA candidate biomarkers. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Clinical Medicine | - |
dc.subject | Aflibercept | - |
dc.subject | Anti-vascular endothelial growth factor | - |
dc.subject | Bevacizumab | - |
dc.subject | Biomarker | - |
dc.subject | Diabetic macular edema | - |
dc.subject | MicroRNA | - |
dc.title | A pilot study on microrna profile in tear fluid to predict response to anti-vegf treatments for diabetic macular edema | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3390/jcm9092920 | - |
dc.identifier.scopus | eid_2-s2.0-85094850424 | - |
dc.identifier.volume | 9 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 18 | - |
dc.identifier.eissn | 2077-0383 | - |
dc.identifier.isi | WOS:000579919900001 | - |