File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Ferulic acid relaxed rat aortic, small mesenteric and coronary arteries by blocking voltage-gated calcium channel and calcium desensitization via dephosphorylation of ERK1/2 and MYPT1

TitleFerulic acid relaxed rat aortic, small mesenteric and coronary arteries by blocking voltage-gated calcium channel and calcium desensitization via dephosphorylation of ERK1/2 and MYPT1
Authors
KeywordsCalcium channel
Calcium sensitization
Endothelium
Ferulic acid
Vasorelaxation
Issue Date2017
Citation
European Journal of Pharmacology, 2017, v. 815, p. 26-32 How to Cite?
AbstractFerulic acid, a natural ingredient presents in several Chinese Materia Medica such as Radix Angelicae Sinensis, has been identified as an important multifunctional and physiologically active small molecule. However, its pharmacological activity in different blood vessel types and underlying mechanisms are unclear. The present study was to investigate the vascular reactivity and the possible action mechanism of FA on aorta, small mesenteric arteries and coronary arteries isolated from Wistar rats. We found FA dose-dependently relieved the contraction of aorta, small mesenteric arteries and coronary arteries induced by different contractors, U46619, phenylephrine (Phe) and KCl. The relaxant effect of FA was not affected by L-NAME (eNOS inhibitor), ODQ (soluble guanylate cyclase inhibitor), and mechanical removal of endothelium in thoracic aortas. The contraction caused by 60 mM KCl (60 K) was concentration-dependently hindered by FA pretreatment in all three types of arteries. In Ca2+-free 60 K solution, FA weakened Ca2+-related contraction in a concentration dependent manner. And FA relaxed both fluoride and phorbol ester which were PKC, ERK and Rho-kinase activators induced contraction in aortic rings with or without Ca2+ in krebs solution. Western blotting experiments in A7r5 cells revealed that FA inhibited calcium sensitization via dephosphorylation of ERK1/2 and MYPT1. Furthermore, the relaxation effect of FA was attenuated by verapamil (calcium channel blocker), ERK inhibitor, and fasudil (ROCK inhibitor). These results provide evidence that FA exhibits endothelium-independent vascular relaxant effect in different types of arteries. The molecular mechanism of vasorelaxation activity of FA probably involved calcium channel inhibition and calcium desensitization.
Persistent Identifierhttp://hdl.handle.net/10722/335813
ISSN
2023 Impact Factor: 4.2
2023 SCImago Journal Rankings: 1.055
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, Zhong Yan-
dc.contributor.authorXu, Jia Qi-
dc.contributor.authorZhao, Wai Rong-
dc.contributor.authorChen, Xin Lin-
dc.contributor.authorJin, Yu-
dc.contributor.authorTang, Nuo-
dc.contributor.authorTang, Jing Yi-
dc.date.accessioned2023-12-28T08:48:56Z-
dc.date.available2023-12-28T08:48:56Z-
dc.date.issued2017-
dc.identifier.citationEuropean Journal of Pharmacology, 2017, v. 815, p. 26-32-
dc.identifier.issn0014-2999-
dc.identifier.urihttp://hdl.handle.net/10722/335813-
dc.description.abstractFerulic acid, a natural ingredient presents in several Chinese Materia Medica such as Radix Angelicae Sinensis, has been identified as an important multifunctional and physiologically active small molecule. However, its pharmacological activity in different blood vessel types and underlying mechanisms are unclear. The present study was to investigate the vascular reactivity and the possible action mechanism of FA on aorta, small mesenteric arteries and coronary arteries isolated from Wistar rats. We found FA dose-dependently relieved the contraction of aorta, small mesenteric arteries and coronary arteries induced by different contractors, U46619, phenylephrine (Phe) and KCl. The relaxant effect of FA was not affected by L-NAME (eNOS inhibitor), ODQ (soluble guanylate cyclase inhibitor), and mechanical removal of endothelium in thoracic aortas. The contraction caused by 60 mM KCl (60 K) was concentration-dependently hindered by FA pretreatment in all three types of arteries. In Ca2+-free 60 K solution, FA weakened Ca2+-related contraction in a concentration dependent manner. And FA relaxed both fluoride and phorbol ester which were PKC, ERK and Rho-kinase activators induced contraction in aortic rings with or without Ca2+ in krebs solution. Western blotting experiments in A7r5 cells revealed that FA inhibited calcium sensitization via dephosphorylation of ERK1/2 and MYPT1. Furthermore, the relaxation effect of FA was attenuated by verapamil (calcium channel blocker), ERK inhibitor, and fasudil (ROCK inhibitor). These results provide evidence that FA exhibits endothelium-independent vascular relaxant effect in different types of arteries. The molecular mechanism of vasorelaxation activity of FA probably involved calcium channel inhibition and calcium desensitization.-
dc.languageeng-
dc.relation.ispartofEuropean Journal of Pharmacology-
dc.subjectCalcium channel-
dc.subjectCalcium sensitization-
dc.subjectEndothelium-
dc.subjectFerulic acid-
dc.subjectVasorelaxation-
dc.titleFerulic acid relaxed rat aortic, small mesenteric and coronary arteries by blocking voltage-gated calcium channel and calcium desensitization via dephosphorylation of ERK1/2 and MYPT1-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ejphar.2017.10.008-
dc.identifier.pmid28989085-
dc.identifier.scopuseid_2-s2.0-85031508040-
dc.identifier.volume815-
dc.identifier.spage26-
dc.identifier.epage32-
dc.identifier.eissn1879-0712-
dc.identifier.isiWOS:000413780700004-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats