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Conference Paper: Enhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents

TitleEnhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents
Authors
Issue Date2009
Citation
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2009, v. 7177, article no. 71772K How to Cite?
AbstractPhotoacoustic imaging of living subjects offers high spatial resolution at increased tissue depths compared to purely optical imaging techniques. We have recently shown that intravenously injected single walled carbon nanotubes (SWNTs) can be used as targeted photoacoustic imaging agents in living mice using RGD peptides to target αvβ3 integrins. We have now developed a new targeted photoacoustic imaging agent based on SWNTs and Indocyanine Green (SWNT-ICG) with absorption peak at 780nm. The photoacoustic signal of the new imaging agent is enhanced by ∼20 times as compared to plain SWNTs. The particles are synthesized from SWNT-RGD that non-covalently attach to multiple ICG molecules through pi-pi stacking interactions. Negative control particles had RAD peptide instead of RGD. We measured the serum stability of the particles and verified that the RGD/RAD conjugation did not alter the particle's absorbance spectrum. Finally, through cell uptake studies with U87MG cells we verified that the particles bind selectively to αvβ3integrin. In conclusion, the extremely high absorption of the SWNT-ICG particles shows great promise for high sensitivity photoacoustic imaging of molecular targets in-vivo. This work lays the foundations for future in-vivo studies that will use the SWNT-ICG particles as imaging agents administered systeMically. © 2009 SPIE.
Persistent Identifierhttp://hdl.handle.net/10722/334987
ISSN
2023 SCImago Journal Rankings: 0.226
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDe La Zerda, Adam-
dc.contributor.authorLiu, Zhuang-
dc.contributor.authorZavaleta, Cristina-
dc.contributor.authorBodapati, Sunil-
dc.contributor.authorTeed, Robert-
dc.contributor.authorVaithilingam, Srikant-
dc.contributor.authorMa, Te Jen-
dc.contributor.authorOralkan, Omer-
dc.contributor.authorChen, Xiaoyuan-
dc.contributor.authorKhuri-Yakub, Butrus T.-
dc.contributor.authorDai, Hongjie-
dc.contributor.authorGambhir, Sanjiv S.-
dc.date.accessioned2023-10-20T06:52:15Z-
dc.date.available2023-10-20T06:52:15Z-
dc.date.issued2009-
dc.identifier.citationProgress in Biomedical Optics and Imaging - Proceedings of SPIE, 2009, v. 7177, article no. 71772K-
dc.identifier.issn1605-7422-
dc.identifier.urihttp://hdl.handle.net/10722/334987-
dc.description.abstractPhotoacoustic imaging of living subjects offers high spatial resolution at increased tissue depths compared to purely optical imaging techniques. We have recently shown that intravenously injected single walled carbon nanotubes (SWNTs) can be used as targeted photoacoustic imaging agents in living mice using RGD peptides to target αvβ3 integrins. We have now developed a new targeted photoacoustic imaging agent based on SWNTs and Indocyanine Green (SWNT-ICG) with absorption peak at 780nm. The photoacoustic signal of the new imaging agent is enhanced by ∼20 times as compared to plain SWNTs. The particles are synthesized from SWNT-RGD that non-covalently attach to multiple ICG molecules through pi-pi stacking interactions. Negative control particles had RAD peptide instead of RGD. We measured the serum stability of the particles and verified that the RGD/RAD conjugation did not alter the particle's absorbance spectrum. Finally, through cell uptake studies with U87MG cells we verified that the particles bind selectively to αvβ3integrin. In conclusion, the extremely high absorption of the SWNT-ICG particles shows great promise for high sensitivity photoacoustic imaging of molecular targets in-vivo. This work lays the foundations for future in-vivo studies that will use the SWNT-ICG particles as imaging agents administered systeMically. © 2009 SPIE.-
dc.languageeng-
dc.relation.ispartofProgress in Biomedical Optics and Imaging - Proceedings of SPIE-
dc.titleEnhanced sensitivity carbon nanotubes as targeted photoacoustic molecular imaging agents-
dc.typeConference_Paper-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1117/12.809601-
dc.identifier.scopuseid_2-s2.0-66249089491-
dc.identifier.volume7177-
dc.identifier.spagearticle no. 71772K-
dc.identifier.epagearticle no. 71772K-
dc.identifier.isiWOS:000285714100080-

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