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Article: Shortwave-infrared-light-emitting probes for the in vivo tracking of cancer vaccines and the elicited immune responses

TitleShortwave-infrared-light-emitting probes for the in vivo tracking of cancer vaccines and the elicited immune responses
Authors
Issue Date2023
Citation
Nature Biomedical Engineering, 2023 How to Cite?
AbstractTracking and imaging immune cells in vivo non-invasively would offer insights into the immune responses induced by vaccination. Here we report a cancer vaccine consisting of polymer-coated NaErF4/NaYF4 core–shell down-conversion nanoparticles emitting luminescence in the near-infrared spectral window IIb (1,500–1,700 nm in wavelength) and with surface-conjugated antigen (ovalbumin) and electrostatically complexed adjuvant (class-B cytosine–phosphate–guanine). Whole-body wide-field imaging of the subcutaneously injected vaccine in tumour-bearing mice revealed rapid migration of the nanoparticles to lymph nodes through lymphatic vessels, with two doses of the vaccine leading to the complete eradication of pre-existing tumours and to the prophylactic inhibition of tumour growth. The abundance of antigen-specific CD8+ T lymphocytes in the tumour microenvironment correlated with vaccine efficacy, as we show via continuous-wave imaging and lifetime imaging of two intravenously injected near-infrared-emitting probes (CD8+-T-cell-targeted NaYbF4/NaYF4 nanoparticles and H-2Kb/ovalbumin257-264 tetramer/PbS/CdS quantum dots) excited at different wavelengths, and by volumetrically visualizing the three nanoparticles via light-sheet microscopy with structured illumination. Nanoparticle-based vaccines and imaging probes emitting infrared light may facilitate the design and optimization of immunotherapies.
Persistent Identifierhttp://hdl.handle.net/10722/334976
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRen, Fuqiang-
dc.contributor.authorWang, Feifei-
dc.contributor.authorBaghdasaryan, Ani-
dc.contributor.authorLi, Ying-
dc.contributor.authorLiu, Haoran-
dc.contributor.authorHsu, Ru Siou-
dc.contributor.authorWang, Chuchu-
dc.contributor.authorLi, Jiachen-
dc.contributor.authorZhong, Yeteng-
dc.contributor.authorSalazar, Felix-
dc.contributor.authorXu, Chun-
dc.contributor.authorJiang, Yingying-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorZhu, Guanzhou-
dc.contributor.authorZhao, Xiang-
dc.contributor.authorWong, Kerry Kaili-
dc.contributor.authorWillis, Richard-
dc.contributor.authorChristopher Garcia, K.-
dc.contributor.authorWu, Anna-
dc.contributor.authorMellins, Elizabeth-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-10-20T06:52:08Z-
dc.date.available2023-10-20T06:52:08Z-
dc.date.issued2023-
dc.identifier.citationNature Biomedical Engineering, 2023-
dc.identifier.urihttp://hdl.handle.net/10722/334976-
dc.description.abstractTracking and imaging immune cells in vivo non-invasively would offer insights into the immune responses induced by vaccination. Here we report a cancer vaccine consisting of polymer-coated NaErF4/NaYF4 core–shell down-conversion nanoparticles emitting luminescence in the near-infrared spectral window IIb (1,500–1,700 nm in wavelength) and with surface-conjugated antigen (ovalbumin) and electrostatically complexed adjuvant (class-B cytosine–phosphate–guanine). Whole-body wide-field imaging of the subcutaneously injected vaccine in tumour-bearing mice revealed rapid migration of the nanoparticles to lymph nodes through lymphatic vessels, with two doses of the vaccine leading to the complete eradication of pre-existing tumours and to the prophylactic inhibition of tumour growth. The abundance of antigen-specific CD8+ T lymphocytes in the tumour microenvironment correlated with vaccine efficacy, as we show via continuous-wave imaging and lifetime imaging of two intravenously injected near-infrared-emitting probes (CD8+-T-cell-targeted NaYbF4/NaYF4 nanoparticles and H-2Kb/ovalbumin257-264 tetramer/PbS/CdS quantum dots) excited at different wavelengths, and by volumetrically visualizing the three nanoparticles via light-sheet microscopy with structured illumination. Nanoparticle-based vaccines and imaging probes emitting infrared light may facilitate the design and optimization of immunotherapies.-
dc.languageeng-
dc.relation.ispartofNature Biomedical Engineering-
dc.titleShortwave-infrared-light-emitting probes for the in vivo tracking of cancer vaccines and the elicited immune responses-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41551-023-01083-5-
dc.identifier.scopuseid_2-s2.0-85168575866-
dc.identifier.eissn2157-846X-
dc.identifier.isiWOS:001059887300001-

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