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- Publisher Website: 10.1038/s41551-020-00642-4
- Scopus: eid_2-s2.0-85094190655
- PMID: 33122853
- WOS: WOS:000582833300002
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Article: Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates
Title | Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates |
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Authors | |
Issue Date | 2020 |
Citation | Nature Biomedical Engineering, 2020, v. 4, n. 12, p. 1188-1196 How to Cite? |
Abstract | Accurate assays for the detection of antibodies to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) are essential for the control of the COVID-19 (coronavirus disease 2019) pandemic. Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates, for the simultaneous detection of antibodies to the S1 subunit of the spike protein and to the receptor binding domain of SARS-CoV-2 in human serum and saliva, and for quantifying immunoglobulin avidities against coronavirus antigens from SARS-CoV-2, SARS-CoV-1 and the common-cold viruses OC43, HKU1, NL63 and 229E. The antibody assay detected immunoglobulin M in 87% (52 of 60) COVID-19-positive serum samples collected 6 or more days after symptom onset (and the immunoglobulins M and G in all 33 samples collected at least 15 days after symptom onset), and correctly classified 456 out of the 457 COVID-19-negative serum samples tested (424 of them collected before the pandemic, including 73 that were positive for other viruses). We used the antibody-avidity assay to study antibody-maturation patterns, anamnestic responses, and cross-immunity to the common-cold coronaviruses. |
Persistent Identifier | http://hdl.handle.net/10722/334696 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Liu, Tiancheng | - |
dc.contributor.author | Hsiung, Jessica | - |
dc.contributor.author | Zhao, Su | - |
dc.contributor.author | Kost, Jessica | - |
dc.contributor.author | Sreedhar, Deepika | - |
dc.contributor.author | Hanson, Carl V. | - |
dc.contributor.author | Olson, Kjerstie | - |
dc.contributor.author | Keare, Douglas | - |
dc.contributor.author | Chang, Shin Ting | - |
dc.contributor.author | Bliden, Kevin P. | - |
dc.contributor.author | Gurbel, Paul A. | - |
dc.contributor.author | Tantry, Udaya S. | - |
dc.contributor.author | Roche, John | - |
dc.contributor.author | Press, Cynthia | - |
dc.contributor.author | Boggs, John | - |
dc.contributor.author | Rodriguez-Soto, Jorge P. | - |
dc.contributor.author | Montoya, Jose G. | - |
dc.contributor.author | Tang, Meijie | - |
dc.contributor.author | Dai, Hongjie | - |
dc.date.accessioned | 2023-10-20T06:50:00Z | - |
dc.date.available | 2023-10-20T06:50:00Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Nature Biomedical Engineering, 2020, v. 4, n. 12, p. 1188-1196 | - |
dc.identifier.uri | http://hdl.handle.net/10722/334696 | - |
dc.description.abstract | Accurate assays for the detection of antibodies to SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) are essential for the control of the COVID-19 (coronavirus disease 2019) pandemic. Here, we report antibody and antibody-avidity assays, relying on near-infrared-fluorescence amplification by nanostructured plasmonic gold substrates, for the simultaneous detection of antibodies to the S1 subunit of the spike protein and to the receptor binding domain of SARS-CoV-2 in human serum and saliva, and for quantifying immunoglobulin avidities against coronavirus antigens from SARS-CoV-2, SARS-CoV-1 and the common-cold viruses OC43, HKU1, NL63 and 229E. The antibody assay detected immunoglobulin M in 87% (52 of 60) COVID-19-positive serum samples collected 6 or more days after symptom onset (and the immunoglobulins M and G in all 33 samples collected at least 15 days after symptom onset), and correctly classified 456 out of the 457 COVID-19-negative serum samples tested (424 of them collected before the pandemic, including 73 that were positive for other viruses). We used the antibody-avidity assay to study antibody-maturation patterns, anamnestic responses, and cross-immunity to the common-cold coronaviruses. | - |
dc.language | eng | - |
dc.relation.ispartof | Nature Biomedical Engineering | - |
dc.title | Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1038/s41551-020-00642-4 | - |
dc.identifier.pmid | 33122853 | - |
dc.identifier.scopus | eid_2-s2.0-85094190655 | - |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1188 | - |
dc.identifier.epage | 1196 | - |
dc.identifier.eissn | 2157-846X | - |
dc.identifier.isi | WOS:000582833300002 | - |