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Article: Aligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis

TitleAligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis
Authors
Keywordsangiogenesis
carbon nanotube
induced pluripotent stem cell
ischemia
nanofibrillar
peripheral arterial disease
Issue Date2015
Citation
ACS Nano, 2015, v. 9, n. 7, p. 6900-6908 How to Cite?
AbstractThe objective of this study was to enhance the angiogenic capacity of endothelial cells (ECs) using nanoscale signaling cues from aligned nanofibrillar scaffolds in the setting of tissue ischemia. Thread-like nanofibrillar scaffolds with porous structure were fabricated from aligned-braided membranes generated under shear from liquid crystal collagen solution. Human ECs showed greater outgrowth from aligned scaffolds than from nonpatterned scaffolds. Integrin α1 was in part responsible for the enhanced cellular outgrowth on aligned nanofibrillar scaffolds, as the effect was abrogated by integrin α1 inhibition. To test the efficacy of EC-seeded aligned nanofibrillar scaffolds in improving neovascularization in vivo, the ischemic limbs of mice were treated with EC-seeded aligned nanofibrillar scaffold; EC-seeded nonpatterned scaffold; ECs in saline; aligned nanofibrillar scaffold alone; or no treatment. After 14 days, laser Doppler blood spectroscopy demonstrated significant improvement in blood perfusion recovery when treated with EC-seeded aligned nanofibrillar scaffolds, in comparison to ECs in saline or no treatment. In ischemic hindlimbs treated with scaffolds seeded with human ECs derived from induced pluripotent stem cells (iPSC-ECs), single-walled carbon nanotube (SWNT) fluorophores were systemically delivered to quantify microvascular density after 28 days. Near infrared-II (NIR-II, 1000-1700 nm) imaging of SWNT fluorophores demonstrated that iPSC-EC-seeded aligned scaffolds group showed significantly higher microvascular density than the saline or cells groups. These data suggest that treatment with EC-seeded aligned nanofibrillar scaffolds improved blood perfusion and arteriogenesis, when compared to treatment with cells alone or scaffold alone, and have important implications in the design of therapeutic cell delivery strategies.
Persistent Identifierhttp://hdl.handle.net/10722/334402
ISSN
2023 Impact Factor: 15.8
2023 SCImago Journal Rankings: 4.593
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNakayama, Karina H.-
dc.contributor.authorHong, Guosong-
dc.contributor.authorLee, Jerry C.-
dc.contributor.authorPatel, Jay-
dc.contributor.authorEdwards, Bryan-
dc.contributor.authorZaitseva, Tatiana S.-
dc.contributor.authorPaukshto, Michael V.-
dc.contributor.authorDai, Hongjie-
dc.contributor.authorCooke, John P.-
dc.contributor.authorWoo, Y. Joseph-
dc.contributor.authorHuang, Ngan F.-
dc.date.accessioned2023-10-20T06:47:53Z-
dc.date.available2023-10-20T06:47:53Z-
dc.date.issued2015-
dc.identifier.citationACS Nano, 2015, v. 9, n. 7, p. 6900-6908-
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/334402-
dc.description.abstractThe objective of this study was to enhance the angiogenic capacity of endothelial cells (ECs) using nanoscale signaling cues from aligned nanofibrillar scaffolds in the setting of tissue ischemia. Thread-like nanofibrillar scaffolds with porous structure were fabricated from aligned-braided membranes generated under shear from liquid crystal collagen solution. Human ECs showed greater outgrowth from aligned scaffolds than from nonpatterned scaffolds. Integrin α1 was in part responsible for the enhanced cellular outgrowth on aligned nanofibrillar scaffolds, as the effect was abrogated by integrin α1 inhibition. To test the efficacy of EC-seeded aligned nanofibrillar scaffolds in improving neovascularization in vivo, the ischemic limbs of mice were treated with EC-seeded aligned nanofibrillar scaffold; EC-seeded nonpatterned scaffold; ECs in saline; aligned nanofibrillar scaffold alone; or no treatment. After 14 days, laser Doppler blood spectroscopy demonstrated significant improvement in blood perfusion recovery when treated with EC-seeded aligned nanofibrillar scaffolds, in comparison to ECs in saline or no treatment. In ischemic hindlimbs treated with scaffolds seeded with human ECs derived from induced pluripotent stem cells (iPSC-ECs), single-walled carbon nanotube (SWNT) fluorophores were systemically delivered to quantify microvascular density after 28 days. Near infrared-II (NIR-II, 1000-1700 nm) imaging of SWNT fluorophores demonstrated that iPSC-EC-seeded aligned scaffolds group showed significantly higher microvascular density than the saline or cells groups. These data suggest that treatment with EC-seeded aligned nanofibrillar scaffolds improved blood perfusion and arteriogenesis, when compared to treatment with cells alone or scaffold alone, and have important implications in the design of therapeutic cell delivery strategies.-
dc.languageeng-
dc.relation.ispartofACS Nano-
dc.subjectangiogenesis-
dc.subjectcarbon nanotube-
dc.subjectinduced pluripotent stem cell-
dc.subjectischemia-
dc.subjectnanofibrillar-
dc.subjectperipheral arterial disease-
dc.titleAligned-Braided Nanofibrillar Scaffold with Endothelial Cells Enhances Arteriogenesis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acsnano.5b00545-
dc.identifier.pmid26061869-
dc.identifier.scopuseid_2-s2.0-84941979113-
dc.identifier.volume9-
dc.identifier.issue7-
dc.identifier.spage6900-
dc.identifier.epage6908-
dc.identifier.eissn1936-086X-
dc.identifier.isiWOS:000358823200027-

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