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Article: Multiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence

TitleMultiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence
Authors
Keywordscytokine
microarray
multiplex
near infrared fluorescence
plasmonic
Issue Date2013
Citation
Nano Research, 2013, v. 6, n. 2, p. 113-120 How to Cite?
AbstractProtein microarrays based on fluorescence detection have been widely utilized for high-throughput functional proteomic analysis. However, a drawback of such assays has been low sensitivity and narrow dynamic range, limiting their capabilities, especially for detecting low abundance biological molecules such as cytokines in human samples. Here, we present fluorescence-enhancing microarrays on plasmonic gold films for multiplexed cytokine detection with up to three orders of magnitude higher sensitivity than on conventional nitrocellulose and glass substrates. Cytokine detection on the gold plasmonic substrate is about one to two orders of magnitude more sensitive than enzyme-linked immunosorbent assay (ELISA) and can be multiplexed. A panel of six cytokines (Vascular endothelial growth factor (VEGF), Interleukin 1β (IL-1β), Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interferon γ (IFN-γ), and Tumor necrosis factor (TNF)) were detected in the culture media of cancer cells. This work establishes a new method of high throughput multiplexed cytokine detection with higher sensitivity and dynamic range than ELISA. Graphical abstract: [Figure not available: see fulltext.] © 2012 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.
Persistent Identifierhttp://hdl.handle.net/10722/334310
ISSN
2023 Impact Factor: 9.5
2023 SCImago Journal Rankings: 2.539
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Bo-
dc.contributor.authorPrice, Jordan-
dc.contributor.authorHong, Guosong-
dc.contributor.authorTabakman, Scott M.-
dc.contributor.authorWang, Hailiang-
dc.contributor.authorJarrell, Justin A.-
dc.contributor.authorFeng, Ju-
dc.contributor.authorUtz, Paul J.-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-10-20T06:47:13Z-
dc.date.available2023-10-20T06:47:13Z-
dc.date.issued2013-
dc.identifier.citationNano Research, 2013, v. 6, n. 2, p. 113-120-
dc.identifier.issn1998-0124-
dc.identifier.urihttp://hdl.handle.net/10722/334310-
dc.description.abstractProtein microarrays based on fluorescence detection have been widely utilized for high-throughput functional proteomic analysis. However, a drawback of such assays has been low sensitivity and narrow dynamic range, limiting their capabilities, especially for detecting low abundance biological molecules such as cytokines in human samples. Here, we present fluorescence-enhancing microarrays on plasmonic gold films for multiplexed cytokine detection with up to three orders of magnitude higher sensitivity than on conventional nitrocellulose and glass substrates. Cytokine detection on the gold plasmonic substrate is about one to two orders of magnitude more sensitive than enzyme-linked immunosorbent assay (ELISA) and can be multiplexed. A panel of six cytokines (Vascular endothelial growth factor (VEGF), Interleukin 1β (IL-1β), Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interferon γ (IFN-γ), and Tumor necrosis factor (TNF)) were detected in the culture media of cancer cells. This work establishes a new method of high throughput multiplexed cytokine detection with higher sensitivity and dynamic range than ELISA. Graphical abstract: [Figure not available: see fulltext.] © 2012 Tsinghua University Press and Springer-Verlag Berlin Heidelberg.-
dc.languageeng-
dc.relation.ispartofNano Research-
dc.subjectcytokine-
dc.subjectmicroarray-
dc.subjectmultiplex-
dc.subjectnear infrared fluorescence-
dc.subjectplasmonic-
dc.titleMultiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12274-012-0286-2-
dc.identifier.scopuseid_2-s2.0-84874189585-
dc.identifier.volume6-
dc.identifier.issue2-
dc.identifier.spage113-
dc.identifier.epage120-
dc.identifier.eissn1998-0000-
dc.identifier.isiWOS:000314763300004-

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