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Article: Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

TitlePlasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range
Authors
Issue Date2011
Citation
Nature Communications, 2011, v. 2, n. 1, article no. 466 How to Cite?
AbstractProtein chips are widely used for high-throughput proteomic analysis, but to date, the low sensitivity and narrow dynamic range have limited their capabilities in diagnostics and proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold film with near-infrared fluorescence enhancement of up to 100-fold, extending the dynamic range of protein detection by three orders of magnitude towards the fM regime. We employ plasmonic protein microarrays for the early detection of a cancer biomarker, carcinoembryonic antigen, in the sera of mice bearing a xenograft tumour model. Further, we demonstrate a multiplexed autoantigen array for human autoantibodies implicated in a range of autoimmune diseases with superior signal-to-noise ratios and broader dynamic range compared with commercial nitrocellulose and glass substrates. The high sensitivity, broad dynamic range and easy adaptability of plasmonic protein chips presents new opportunities in proteomic research and diagnostics applications. © 2011 Macmillan Publishers Limited. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/334262
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTabakman, Scott M.-
dc.contributor.authorLau, Lana-
dc.contributor.authorRobinson, Joshua T.-
dc.contributor.authorPrice, Jordan-
dc.contributor.authorSherlock, Sarah P.-
dc.contributor.authorWang, Hailiang-
dc.contributor.authorZhang, Bo-
dc.contributor.authorChen, Zhuo-
dc.contributor.authorTangsombatvisit, Stephanie-
dc.contributor.authorJarrell, Justin A.-
dc.contributor.authorUtz, Paul J.-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-10-20T06:46:52Z-
dc.date.available2023-10-20T06:46:52Z-
dc.date.issued2011-
dc.identifier.citationNature Communications, 2011, v. 2, n. 1, article no. 466-
dc.identifier.urihttp://hdl.handle.net/10722/334262-
dc.description.abstractProtein chips are widely used for high-throughput proteomic analysis, but to date, the low sensitivity and narrow dynamic range have limited their capabilities in diagnostics and proteomics. Here we present protein microarrays on a novel nanostructured, plasmonic gold film with near-infrared fluorescence enhancement of up to 100-fold, extending the dynamic range of protein detection by three orders of magnitude towards the fM regime. We employ plasmonic protein microarrays for the early detection of a cancer biomarker, carcinoembryonic antigen, in the sera of mice bearing a xenograft tumour model. Further, we demonstrate a multiplexed autoantigen array for human autoantibodies implicated in a range of autoimmune diseases with superior signal-to-noise ratios and broader dynamic range compared with commercial nitrocellulose and glass substrates. The high sensitivity, broad dynamic range and easy adaptability of plasmonic protein chips presents new opportunities in proteomic research and diagnostics applications. © 2011 Macmillan Publishers Limited. All rights reserved.-
dc.languageeng-
dc.relation.ispartofNature Communications-
dc.titlePlasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/ncomms1477-
dc.identifier.pmid21915108-
dc.identifier.scopuseid_2-s2.0-80053410118-
dc.identifier.volume2-
dc.identifier.issue1-
dc.identifier.spagearticle no. 466-
dc.identifier.epagearticle no. 466-
dc.identifier.eissn2041-1723-
dc.identifier.isiWOS:000294807200008-

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