File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)

Book Chapter: Aptamers as Functional Modules for DNA Nanostructures

TitleAptamers as Functional Modules for DNA Nanostructures
Authors
KeywordsAptamers
Atomic force microscopy
Bioanalytical sensors
Biophysical assays
Circular dichroism
DNA nanostructures
Droplet microfluidic SELEX
Transmission electron microscopy
Issue Date1-Jan-2023
PublisherHumana Press, Inc.
Abstract

Watson-Crick base-pairing of DNA allows the nanoscale fabrication of biocompatible synthetic nanostructures for diagnostic and therapeutic biomedical purposes. DNA nanostructure design elicits exquisite control of shape and conformation compared to other nanoparticles. Furthermore, nucleic acid aptamers can be coupled to DNA nanostructures to allow interaction and response to a plethora of biomolecules beyond nucleic acids. When compared to the better-known approach of using protein antibodies for molecular recognition, nucleic acid aptamers are bespoke with the underlying DNA nanostructure backbone and have various other stability, synthesis, and cost advantages. Here, we provide detailed methodologies to synthesize and characterize aptamer-enabled DNA nanostructures. The methods described can be generally applied to various designs of aptamer-enabled DNA nanostructures with a wide range of applications both within and beyond biomedical nanotechnology.


Persistent Identifierhttp://hdl.handle.net/10722/333836
ISSN
2023 SCImago Journal Rankings: 0.399

 

DC FieldValueLanguage
dc.contributor.authorShiu, Simon Chi-Chin-
dc.contributor.authorKinghorn, Andrew B-
dc.contributor.authorGuo, Wei-
dc.contributor.authorSlaughter, Liane S-
dc.contributor.authorJi, Danyang-
dc.contributor.authorMo, Xiaoyong-
dc.contributor.authorWang, Lin-
dc.contributor.authorTran, Ngoc Chau-
dc.contributor.authorKwok, Chun Kit-
dc.contributor.authorShum, Anderson Ho Cheung-
dc.contributor.authorTse, Edmund Chun Ming-
dc.contributor.authorTanner, Julian A-
dc.date.accessioned2023-10-06T08:39:28Z-
dc.date.available2023-10-06T08:39:28Z-
dc.date.issued2023-01-01-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/333836-
dc.description.abstract<p>Watson-Crick base-pairing of DNA allows the nanoscale fabrication of biocompatible synthetic nanostructures for diagnostic and therapeutic biomedical purposes. DNA nanostructure design elicits exquisite control of shape and conformation compared to other nanoparticles. Furthermore, nucleic acid aptamers can be coupled to DNA nanostructures to allow interaction and response to a plethora of biomolecules beyond nucleic acids. When compared to the better-known approach of using protein antibodies for molecular recognition, nucleic acid aptamers are bespoke with the underlying DNA nanostructure backbone and have various other stability, synthesis, and cost advantages. Here, we provide detailed methodologies to synthesize and characterize aptamer-enabled DNA nanostructures. The methods described can be generally applied to various designs of aptamer-enabled DNA nanostructures with a wide range of applications both within and beyond biomedical nanotechnology.<br></p>-
dc.languageeng-
dc.publisherHumana Press, Inc.-
dc.relation.ispartofMethods in molecular biology-
dc.subjectAptamers-
dc.subjectAtomic force microscopy-
dc.subjectBioanalytical sensors-
dc.subjectBiophysical assays-
dc.subjectCircular dichroism-
dc.subjectDNA nanostructures-
dc.subjectDroplet microfluidic SELEX-
dc.subjectTransmission electron microscopy-
dc.titleAptamers as Functional Modules for DNA Nanostructures-
dc.typeBook_Chapter-
dc.identifier.doi10.1007/978-1-0716-3028-0_17-
dc.identifier.scopuseid_2-s2.0-85159469670-
dc.identifier.volume2639-
dc.identifier.spage301-
dc.identifier.epage337-
dc.identifier.eissn1940-6029-
dc.identifier.issnl1064-3745-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats