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Article: Extracellular vesicles from medicated plasma of Buyang Huanwu decoction-preconditioned neural stem cells accelerate neurological recovery following ischemic stroke

TitleExtracellular vesicles from medicated plasma of Buyang Huanwu decoction-preconditioned neural stem cells accelerate neurological recovery following ischemic stroke
Authors
KeywordsBuyang Huanwu decoction
extracellular vesicles
ischemic stroke
medicated plasma
miRNAs profiles
neural stem cells
neurogenesis
Issue Date1-Mar-2023
PublisherFrontiers Media
Citation
Frontiers in Bioengineering and Biotechnology, 2023, v. 11 How to Cite?
Abstract

Introduction: The neurological impairment of survivors after ischemic stroke poses a serious risk to their quality of life and health. Effective therapeutic options are still lacking. Neural stem cells (NSCs) promote neurogenesis via secreted extracellular vesicles (NSC-EVs), which would be a potential therapeutic option, but the insufficient quantity of NSC-EVs in vivo restrains clinical application. Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) decoction, is promising to alleviate neurological impairment after ischemic stroke. It was speculated that BHD might promote neurological recovery through the NSC-EVs.

Methods: The medicated plasma of BHD (MP-BHD) was prepared to precondition NSCs and isolate EVs (BHD-NSC-EVs). Middle cerebral artery occlusion (MCAO) models and primary NSCs were administered to evaluate the therapeutic effect. Next-generation sequencing was performed to explore the mechanism.

Results: The BHD-NSC-EVs more significantly accelerated neurological recovery after MCAO and promoted NSCs proliferation and differentiation than BHD and NSC-EVs alone. MP-BHD enhanced the largescale generation of BHD-NSC-EVs, which encapsulated functional miRNA and may play critical roles in neurogenesis.

Discussion: In replacing BHD or NSCs, the preconditioned NSC-EVs present a more efficient therapeutic strategy for ischemic stroke. Based on the clinical efficacy of TCM, the preconditioning of NSC-derived EVs via the MP of TCM herbs would presents a newly promising therapeutic strategy for neurological diseases.


Persistent Identifierhttp://hdl.handle.net/10722/332222
ISSN
2023 Impact Factor: 4.3
2023 SCImago Journal Rankings: 0.893
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLong, J-
dc.contributor.authorGu, CY-
dc.contributor.authorZhang, QK-
dc.contributor.authorLiu, JL-
dc.contributor.authorHuang, JJ-
dc.contributor.authorLi, YJ-
dc.contributor.authorZhang, YF-
dc.contributor.authorLi, R-
dc.contributor.authorAhmed, W-
dc.contributor.authorZhang, JF-
dc.contributor.authorKhan, AA-
dc.contributor.authorCai, HS-
dc.contributor.authorHu, Y-
dc.contributor.authorChen, LK-
dc.date.accessioned2023-10-04T07:21:01Z-
dc.date.available2023-10-04T07:21:01Z-
dc.date.issued2023-03-01-
dc.identifier.citationFrontiers in Bioengineering and Biotechnology, 2023, v. 11-
dc.identifier.issn2296-4185-
dc.identifier.urihttp://hdl.handle.net/10722/332222-
dc.description.abstract<p><strong>Introduction:</strong> The neurological impairment of survivors after ischemic stroke poses a serious risk to their quality of life and health. Effective therapeutic options are still lacking. Neural stem cells (NSCs) promote neurogenesis <em>via</em> secreted extracellular vesicles (NSC-EVs), which would be a potential therapeutic option, but the insufficient quantity of NSC-EVs <em>in vivo</em> restrains clinical application. Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) decoction, is promising to alleviate neurological impairment after ischemic stroke. It was speculated that BHD might promote neurological recovery through the NSC-EVs.</p><p><strong>Methods:</strong> The medicated plasma of BHD (MP-BHD) was prepared to precondition NSCs and isolate EVs (BHD-NSC-EVs). Middle cerebral artery occlusion (MCAO) models and primary NSCs were administered to evaluate the therapeutic effect. Next-generation sequencing was performed to explore the mechanism.</p><p><strong>Results:</strong> The BHD-NSC-EVs more significantly accelerated neurological recovery after MCAO and promoted NSCs proliferation and differentiation than BHD and NSC-EVs alone. MP-BHD enhanced the largescale generation of BHD-NSC-EVs, which encapsulated functional miRNA and may play critical roles in neurogenesis.</p><p><strong>Discussion:</strong> In replacing BHD or NSCs, the preconditioned NSC-EVs present a more efficient therapeutic strategy for ischemic stroke. Based on the clinical efficacy of TCM, the preconditioning of NSC-derived EVs <em>via</em> the MP of TCM herbs would presents a newly promising therapeutic strategy for neurological diseases.</p>-
dc.languageeng-
dc.publisherFrontiers Media-
dc.relation.ispartofFrontiers in Bioengineering and Biotechnology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectBuyang Huanwu decoction-
dc.subjectextracellular vesicles-
dc.subjectischemic stroke-
dc.subjectmedicated plasma-
dc.subjectmiRNAs profiles-
dc.subjectneural stem cells-
dc.subjectneurogenesis-
dc.titleExtracellular vesicles from medicated plasma of Buyang Huanwu decoction-preconditioned neural stem cells accelerate neurological recovery following ischemic stroke-
dc.typeArticle-
dc.identifier.doi10.3389/fcell.2023.1096329-
dc.identifier.scopuseid_2-s2.0-85150215022-
dc.identifier.volume11-
dc.identifier.eissn2296-4185-
dc.identifier.isiWOS:000951854200001-
dc.identifier.issnl2296-4185-

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