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Article: Evaluation of a Rapid Immunochromographic Middle East Respiratory Syndrome Coronavirus Antigen Detection Assay

TitleEvaluation of a Rapid Immunochromographic Middle East Respiratory Syndrome Coronavirus Antigen Detection Assay
Authors
Keywordsdromedary camels
MERS-CoV
rapid antigen detection assay
Issue Date1-Dec-2022
PublisherLippincott, Williams & Wilkins
Citation
Infectious Microbes & Diseases, 2022, v. 4(4), n. 4, p. 175-177 How to Cite?
Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) infection in humans has a high mortality of >30%. Dromedaries are the reservoir of MERS-CoV and the main source of human infections. However, MERS-CoV infections in dromedaries are usually subclinical. Rapid diagnosis of MERS-CoV infection in these animals is important in preventing camel-to-human transmission of the virus. The possible cross-reactivity of a previously reported rapid nucleocapsid protein-based antigen detection assay for MERS-CoV was examined with different CoVs, including Tylonycteris bat CoV HKU4, dromedary camel CoV UAE-HKU23, human CoV-229E, human CoV-OC43, severe acute respiratory syndrome CoV-2 and rabbit CoV HKU14, where none of them showed false-positive results. The assay was further validated using quantitative real-time reverse transcription-polymerase chain reaction–confirmed MERS-CoV–positive and MERS-CoV–negative dromedary nasal samples collected in Dubai, the United Arab Emirates, which showed that the rapid antigen detection assay has a specificity of 100% and sensitivity of 91.7%.


Persistent Identifierhttp://hdl.handle.net/10722/331544
ISSN
2023 Impact Factor: 2.0
2023 SCImago Journal Rankings: 0.307
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, SK-
dc.contributor.authorLi, KSM-
dc.contributor.authorTeng, JL-
dc.contributor.authorJoseph, Sunitha-
dc.contributor.authorLuk, HKH-
dc.contributor.authorFung, J-
dc.contributor.authorWernery, U-
dc.contributor.authorWoo, PC-
dc.date.accessioned2023-09-21T06:56:47Z-
dc.date.available2023-09-21T06:56:47Z-
dc.date.issued2022-12-01-
dc.identifier.citationInfectious Microbes & Diseases, 2022, v. 4(4), n. 4, p. 175-177-
dc.identifier.issn2641-5917-
dc.identifier.urihttp://hdl.handle.net/10722/331544-
dc.description.abstract<p>Middle East respiratory syndrome coronavirus (MERS-CoV) infection in humans has a high mortality of >30%. Dromedaries are the reservoir of MERS-CoV and the main source of human infections. However, MERS-CoV infections in dromedaries are usually subclinical. Rapid diagnosis of MERS-CoV infection in these animals is important in preventing camel-to-human transmission of the virus. The possible cross-reactivity of a previously reported rapid nucleocapsid protein-based antigen detection assay for MERS-CoV was examined with different CoVs, including <em>Tylonycteris</em> bat CoV HKU4, dromedary camel CoV UAE-HKU23, human CoV-229E, human CoV-OC43, severe acute respiratory syndrome CoV-2 and rabbit CoV HKU14, where none of them showed false-positive results. The assay was further validated using quantitative real-time reverse transcription-polymerase chain reaction–confirmed MERS-CoV–positive and MERS-CoV–negative dromedary nasal samples collected in Dubai, the United Arab Emirates, which showed that the rapid antigen detection assay has a specificity of 100% and sensitivity of 91.7%.<br></p>-
dc.languageeng-
dc.publisherLippincott, Williams & Wilkins-
dc.relation.ispartofInfectious Microbes & Diseases-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectdromedary camels-
dc.subjectMERS-CoV-
dc.subjectrapid antigen detection assay-
dc.titleEvaluation of a Rapid Immunochromographic Middle East Respiratory Syndrome Coronavirus Antigen Detection Assay-
dc.typeArticle-
dc.identifier.doi10.1097/IM9.0000000000000105-
dc.identifier.scopuseid_2-s2.0-85144883461-
dc.identifier.volume4(4)-
dc.identifier.issue4-
dc.identifier.spage175-
dc.identifier.epage177-
dc.identifier.eissn2641-5917-
dc.identifier.isiWOS:000901813100007-
dc.identifier.issnl2641-5917-

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