MiR-200b-5p inhibits tumor progression in salivary adenoid cystic carcinoma via targeting BTBD1

Abstract

<p>Salivary adenoid cystic carcinoma (SACC) is a rare malignant tumor of the salivary gland. Studies have suggested that miRNA may play a crucial role in the invasion and metastasis of SACC. This study aimed to investigate the role of miR-200b-5p in SACC progression. Reverse transcription-quantitative PCR and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/western-blot" title="Learn more about western blot from ScienceDirect's AI-generated Topic Pages">western blot</a> assay were used to detect the expression levels of miR-200b-5p and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/btb-poz-domain" title="Learn more about BTBD1 from ScienceDirect's AI-generated Topic Pages">BTBD1</a>. The biological functions of miR-200b-5p were evaluated via wound-healing assays, transwell assays, and xenograft <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/nude-mouse" title="Learn more about nude mice from ScienceDirect's AI-generated Topic Pages">nude mice</a> model. The interaction between miR-200b-5p and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/btb-poz-domain" title="Learn more about BTBD1 from ScienceDirect's AI-generated Topic Pages">BTBD1</a> was assessed using <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/luciferase" title="Learn more about luciferase from ScienceDirect's AI-generated Topic Pages">luciferase</a> assay. Results showed that miR-200b-5p was downregulated in the SACC tissues while BTBD1 was upregulated. miR-200b-5p overexpression suppressed SACC <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/cell-proliferation" title="Learn more about cell proliferation from ScienceDirect's AI-generated Topic Pages">cell proliferation</a>, migration, invasion, and epithelial-mesenchymal transition (EMT). Bioinformatics prediction and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/luciferase" title="Learn more about luciferase from ScienceDirect's AI-generated Topic Pages">luciferase</a> reporter assay revealed that miR-200b-5p could directly bind to BTBD1. Besides, miR-200b-5p overexpression could rescue the tumor-promoting effect of BTBD1. miR-200b-5p inhibited tumor progression by modulating EMT-related proteins, targeting BTBD1 and inhibiting PI3K/AKT <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/signal-transduction" title="Learn more about signaling pathway from ScienceDirect's AI-generated Topic Pages">signaling pathway</a>. Overall, our findings indicate that miR-200b-5p can suppress SACC proliferation, migration, invasion, and EMT by regulating BTBD1 and PI3K/AKT axis, providing a promising therapeutic target for SACC treatment.</p>