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Article: Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors

TitleTargeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors
Authors
Keywordscalcium/calmodulin-dependent protein kinase 2 isoform A
cancer stem cells
glutathione S-transferase pi
lung adenocarcinoma
nuclear factor erythroid 2-related factor 2
Issue Date31-Mar-2023
PublisherWiley Open Access
Citation
Advanced Science, 2023, v. 10, n. 7 How to Cite?
AbstractGlutathione S-transferase pi (GSTP1), a phase II detoxification enzyme, is known to be overexpressed and mediates chemotherapeutic resistance in lung cancer. However, whether GSTP1 supports cancer stem cells (CSCs) and the underlying mechanisms in lung adenocarcinoma (LUAD) remain largely unknown. This study unveiled that GSTP1 is upregulated in lung CSCs and supports tumor self-renewal, metastasis, and resistance to targeted tyrosine kinase inhibitors of LUAD both in vitro and in vivo. Mechanistically, CaMK2A (calcium/calmodulin-dependent protein kinase 2 isoform A)/NRF2 (nuclear factor erythroid 2-related factor 2)/GSTP1 is uncovered as a regulatory axis under hypoxia. CaMK2A increased GSTP1 expression through phosphorylating the Sersine558 residue of NRF2 and promoting its nuclear translocation, a novel mechanism for NRF2 activation apart from conventional oxidization-dependent activation. Upregulation of GSTP1 in turn suppressed reactive oxygen species levels and supported CSC phenotypes. Clinically, GSTP1 analyzed by immunohistochemistry is upregulated in a proportion of LUAD and serves as a prognostic marker for survival. Using patient-derived organoids from an ALK-translocated LUAD, the therapeutic potential of a specific GSTP1 inhibitor ezatiostat in combination treatment with the ALK inhibitor crizotinib is demonstrated. This study demonstrates GSTP1 to be a promising therapeutic target for long-term control of LUAD through targeting CSCs.
Persistent Identifierhttp://hdl.handle.net/10722/331272
ISSN
2023 Impact Factor: 14.3
2023 SCImago Journal Rankings: 3.914
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, SQ-
dc.contributor.authorChen, JJ-
dc.contributor.authorJiang, Y-
dc.contributor.authorLei, ZN-
dc.contributor.authorRuan, YC-
dc.contributor.authorPan, Y-
dc.contributor.authorYam, JWP-
dc.contributor.authorWong, MP-
dc.contributor.authorXiao, ZJ-
dc.date.accessioned2023-09-21T06:54:14Z-
dc.date.available2023-09-21T06:54:14Z-
dc.date.issued2023-03-31-
dc.identifier.citationAdvanced Science, 2023, v. 10, n. 7-
dc.identifier.issn2198-3844-
dc.identifier.urihttp://hdl.handle.net/10722/331272-
dc.description.abstractGlutathione S-transferase pi (GSTP1), a phase II detoxification enzyme, is known to be overexpressed and mediates chemotherapeutic resistance in lung cancer. However, whether GSTP1 supports cancer stem cells (CSCs) and the underlying mechanisms in lung adenocarcinoma (LUAD) remain largely unknown. This study unveiled that GSTP1 is upregulated in lung CSCs and supports tumor self-renewal, metastasis, and resistance to targeted tyrosine kinase inhibitors of LUAD both in vitro and in vivo. Mechanistically, CaMK2A (calcium/calmodulin-dependent protein kinase 2 isoform A)/NRF2 (nuclear factor erythroid 2-related factor 2)/GSTP1 is uncovered as a regulatory axis under hypoxia. CaMK2A increased GSTP1 expression through phosphorylating the Sersine558 residue of NRF2 and promoting its nuclear translocation, a novel mechanism for NRF2 activation apart from conventional oxidization-dependent activation. Upregulation of GSTP1 in turn suppressed reactive oxygen species levels and supported CSC phenotypes. Clinically, GSTP1 analyzed by immunohistochemistry is upregulated in a proportion of LUAD and serves as a prognostic marker for survival. Using patient-derived organoids from an ALK-translocated LUAD, the therapeutic potential of a specific GSTP1 inhibitor ezatiostat in combination treatment with the ALK inhibitor crizotinib is demonstrated. This study demonstrates GSTP1 to be a promising therapeutic target for long-term control of LUAD through targeting CSCs.-
dc.languageeng-
dc.publisherWiley Open Access-
dc.relation.ispartofAdvanced Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectcalcium/calmodulin-dependent protein kinase 2 isoform A-
dc.subjectcancer stem cells-
dc.subjectglutathione S-transferase pi-
dc.subjectlung adenocarcinoma-
dc.subjectnuclear factor erythroid 2-related factor 2-
dc.titleTargeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors-
dc.typeArticle-
dc.identifier.doi10.1002/advs.202205262-
dc.identifier.pmid36709476-
dc.identifier.scopuseid_2-s2.0-85147271077-
dc.identifier.volume10-
dc.identifier.issue7-
dc.identifier.eissn2198-3844-
dc.identifier.isiWOS:000921431300001-
dc.identifier.issnl2198-3844-

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