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Article: Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells

TitleProtocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
Authors
Kong, NannanChan, Ying Wai
KeywordsCell Biology
CRISPR
Issue Date16-Jun-2023
PublisherCell Press
Citation
STAR Protocols, 2023, v. 4, n. 2 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2023.102286
Abstract

We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and designing the sgRNA and homologous repair template, and clone selection and verification. For complete details on the use and execution of this protocol, please refer to Kong et al.


Persistent Identifierhttp://hdl.handle.net/10722/331162
ISSN
2666-1667
2023 Impact Factor: 1.3
2023 SCImago Journal Rankings: 0.955
ISI Accession Number ID
WOS:001094590600001

 

DC FieldValueLanguage
dc.contributor.authorKong, Nannan-
dc.contributor.authorChan, Ying Wai-
dc.date.accessioned2023-09-21T06:53:17Z-
dc.date.available2023-09-21T06:53:17Z-
dc.date.issued2023-06-16-
dc.identifier.citationSTAR Protocols, 2023, v. 4, n. 2-
dc.identifier.issn2666-1667-
dc.identifier.urihttp://hdl.handle.net/10722/331162-
dc.description.abstract<p>We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and designing the sgRNA and homologous repair template, and clone selection and verification. For complete details on the use and execution of this protocol, please refer to Kong et al.<br></p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofSTAR Protocols-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCell Biology-
dc.subjectCRISPR-
dc.titleProtocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1016/j.xpro.2023.102286-
dc.identifier.scopuseid_2-s2.0-85160267059-
dc.identifier.volume4-
dc.identifier.issue2-
dc.identifier.eissn2666-1667-
dc.identifier.isiWOS:001094590600001-
dc.identifier.issnl2666-1667-

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