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Article: Activity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells

TitleActivity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells
Authors
Yau, Wai YinLai, Samuel Kin ManBlasco, PilarLi, XuechenNg, Kwan MingLok, Chun NamAu Yeung, Ho Yu
Issue Date21-Apr-2023
PublisherChemistry Europe
Citation
Analysis & Sensing, 2023 How to Cite?
DOI: http://dx.doi.org/10.1002/anse.202300012
Abstract

Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.


Persistent Identifierhttp://hdl.handle.net/10722/328982
ISSN
2629-2742

 

DC FieldValueLanguage
dc.contributor.authorYau, Wai Yin-
dc.contributor.authorLai, Samuel Kin Man-
dc.contributor.authorBlasco, Pilar-
dc.contributor.authorLi, Xuechen-
dc.contributor.authorNg, Kwan Ming-
dc.contributor.authorLok, Chun Nam-
dc.contributor.authorAu Yeung, Ho Yu-
dc.date.accessioned2023-08-05T07:54:24Z-
dc.date.available2023-08-05T07:54:24Z-
dc.date.issued2023-04-21-
dc.identifier.citationAnalysis & Sensing, 2023-
dc.identifier.issn2629-2742-
dc.identifier.urihttp://hdl.handle.net/10722/328982-
dc.description.abstract<p>Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.</p>-
dc.languageeng-
dc.publisherChemistry Europe-
dc.relation.ispartofAnalysis & Sensing-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleActivity‐based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells-
dc.typeArticle-
dc.identifier.doi10.1002/anse.202300012-
dc.identifier.eissn2629-2742-
dc.identifier.issnl2629-2742-

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