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Article: Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma

TitleIdentification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma
Authors
Li, Mei MeiKong, Fan EnLi, Guang MengHe, Yi TiZhang, Xiao FengZhang, Cheng YangLiang, Jie KaiGuan, Xin YuanMa, Ning FangXie, Mao BinLiu, Ming
Issue Date1-Dec-2022
PublisherCell Press
Citation
STAR Protocols, 2022, v. 3, n. 4 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2022.101921
Abstract

Here, we present a detailed protocol for the identification of potential oncofetal targets for hepatocellular carcinoma (HCC) patients through a hepatocyte differentiation model and a sorafenib refractory cell-line-derived xenograft model. We describe the procedures of tumor sphere formation, organoid generation, and subcutaneous tumor formation for functional studies. We then detail the procedures of immunohistochemistry and immunofluorescence for examination of changes in lineage-specific markers. Finally, we describe the development of antibody-based therapeutics targeting tumor lineage plasticity in HCC.


Persistent Identifierhttp://hdl.handle.net/10722/328536
ISSN
2666-1667
2023 Impact Factor: 1.3
2023 SCImago Journal Rankings: 0.955

 

DC FieldValueLanguage
dc.contributor.authorLi, Mei Mei-
dc.contributor.authorKong, Fan En-
dc.contributor.authorLi, Guang Meng-
dc.contributor.authorHe, Yi Ti-
dc.contributor.authorZhang, Xiao Feng-
dc.contributor.authorZhang, Cheng Yang-
dc.contributor.authorLiang, Jie Kai-
dc.contributor.authorGuan, Xin Yuan-
dc.contributor.authorMa, Ning Fang-
dc.contributor.authorXie, Mao Bin-
dc.contributor.authorLiu, Ming -
dc.date.accessioned2023-06-28T04:45:55Z-
dc.date.available2023-06-28T04:45:55Z-
dc.date.issued2022-12-01-
dc.identifier.citationSTAR Protocols, 2022, v. 3, n. 4-
dc.identifier.issn2666-1667-
dc.identifier.urihttp://hdl.handle.net/10722/328536-
dc.description.abstract<p>Here, we present a detailed protocol for the identification of potential oncofetal targets for hepatocellular carcinoma (HCC) patients through a hepatocyte differentiation model and a sorafenib refractory cell-line-derived xenograft model. We describe the procedures of tumor sphere formation, organoid generation, and subcutaneous tumor formation for functional studies. We then detail the procedures of immunohistochemistry and immunofluorescence for examination of changes in lineage-specific markers. Finally, we describe the development of antibody-based therapeutics targeting tumor lineage plasticity in HCC.<br></p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofSTAR Protocols-
dc.titleIdentification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma-
dc.typeArticle-
dc.identifier.doi10.1016/j.xpro.2022.101921-
dc.identifier.hkuros344667-
dc.identifier.volume3-
dc.identifier.issue4-
dc.identifier.eissn2666-1667-
dc.identifier.issnl2666-1667-

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