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Article: 3D-printed gelatin/sodium alginate/58S bioactive glass scaffolds promote osteogenesis in vitro and in vivo

Title3D-printed gelatin/sodium alginate/58S bioactive glass scaffolds promote osteogenesis in vitro and in vivo
Authors
Issue Date1-Mar-2023
PublisherSAGE Publications
Citation
Journal of Biomaterials Applications, 2023, v. 37, n. 10 How to Cite?
Abstract

Three-dimensional (3D)-printed scaffolds are a new strategy to fabricate biomaterials for treating bone defects. Here, using a 3D-printing technique, we fabricated scaffolds consisting of gelatin (Gel), sodium alginate (SA), and 58S bioactive glass (58S BG). To evaluate mechanical properties and biocompatibility of Gel/SA/58S BG scaffolds, the degradation test, compressive strength test, and cytotoxicity test were performed. The effect of the scaffolds on cell proliferation in vitro was determined by 4′,6-diamidino-2-phenylindole (DAPI) staining. To evaluate osteoinductive properties, rBMSCs were cultured on the scaffolds for 7, 14, and 21 days and the expression of osteogenesis-related genes was analyzed using qRT-PCR. To examine the bone healing properties of Gel/SA/58S BG scaffolds in vivo, we used a rat mandibular critical-size defect bone model. The scaffolds were implanted into the defect area of rat mandible and bone regeneration and new tissue formation were assessed using microcomputed tomography (microCT) and hematoxylin and eosin (H&E) staining. The results showed that Gel/SA/58S BG scaffolds had appropriate mechanical strength as a filling material for bone defects. Furthermore, the scaffolds could be compressed within certain limits and then could recover their shape. The extract of the Gel/SA/58S BG scaffold showed no cytotoxicity. In vitro, the expression levels of Bmp2Runx2, and OCN were increased in rBMSCs cultured on the scaffolds. In vivo, microCT and H&E staining demonstrated that scaffolds induced the formation of new bone at the mandibular defect area. These results indicated that Gel/SA/58S BG scaffolds have excellent mechanical characteristics, biocompatibility, and osteoinductive properties, suggesting that it could be a promising biomaterial for the repair of bone defects.


Persistent Identifierhttp://hdl.handle.net/10722/328348
ISSN
2023 Impact Factor: 2.3
2023 SCImago Journal Rankings: 0.473

 

DC FieldValueLanguage
dc.contributor.authorTu, XR-
dc.contributor.authorGuo, LY-
dc.contributor.authorLi, Y-
dc.contributor.authorTan, GZ-
dc.contributor.authorChen, RF-
dc.contributor.authorWu, JW-
dc.contributor.authorMiao, GH-
dc.contributor.authorGuo, L-
dc.contributor.authorZhang, CF-
dc.contributor.authorZou, T-
dc.contributor.authorZhang, Y-
dc.contributor.authorJiang, QZ -
dc.date.accessioned2023-06-28T04:42:52Z-
dc.date.available2023-06-28T04:42:52Z-
dc.date.issued2023-03-01-
dc.identifier.citationJournal of Biomaterials Applications, 2023, v. 37, n. 10-
dc.identifier.issn0885-3282-
dc.identifier.urihttp://hdl.handle.net/10722/328348-
dc.description.abstract<p>Three-dimensional (3D)-printed scaffolds are a new strategy to fabricate biomaterials for treating bone defects. Here, using a 3D-printing technique, we fabricated scaffolds consisting of gelatin (Gel), sodium alginate (SA), and 58S bioactive glass (58S BG). To evaluate mechanical properties and biocompatibility of Gel/SA/58S BG scaffolds, the degradation test, compressive strength test, and cytotoxicity test were performed. The effect of the scaffolds on cell proliferation in vitro was determined by 4′,6-diamidino-2-phenylindole (DAPI) staining. To evaluate osteoinductive properties, rBMSCs were cultured on the scaffolds for 7, 14, and 21 days and the expression of osteogenesis-related genes was analyzed using qRT-PCR. To examine the bone healing properties of Gel/SA/58S BG scaffolds in vivo, we used a rat mandibular critical-size defect bone model. The scaffolds were implanted into the defect area of rat mandible and bone regeneration and new tissue formation were assessed using microcomputed tomography (microCT) and hematoxylin and eosin (H&E) staining. The results showed that Gel/SA/58S BG scaffolds had appropriate mechanical strength as a filling material for bone defects. Furthermore, the scaffolds could be compressed within certain limits and then could recover their shape. The extract of the Gel/SA/58S BG scaffold showed no cytotoxicity. In vitro, the expression levels of <em>Bmp2</em>, <em>Runx2</em>, and <em>OCN</em> were increased in rBMSCs cultured on the scaffolds. In vivo, microCT and H&E staining demonstrated that scaffolds induced the formation of new bone at the mandibular defect area. These results indicated that Gel/SA/58S BG scaffolds have excellent mechanical characteristics, biocompatibility, and osteoinductive properties, suggesting that it could be a promising biomaterial for the repair of bone defects.<br></p>-
dc.languageeng-
dc.publisherSAGE Publications-
dc.relation.ispartofJournal of Biomaterials Applications-
dc.title3D-printed gelatin/sodium alginate/58S bioactive glass scaffolds promote osteogenesis in vitro and in vivo-
dc.typeArticle-
dc.identifier.doi10.1177/08853282231152128-
dc.identifier.hkuros344694-
dc.identifier.volume37-
dc.identifier.issue10-
dc.identifier.eissn1530-8022-
dc.identifier.issnl0885-3282-

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