File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Article: Menin 'reads' H3K79me2 mark in a nucleosomal context
Title | Menin 'reads' H3K79me2 mark in a nucleosomal context |
---|---|
Authors | |
Issue Date | 2023 |
Citation | Science, 2023, v. 379, p. 717-723 How to Cite? |
Abstract | Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this histone mark is translated into downstream effects remains poorly understood owing to a lack of knowledge about its readers. We developed a nucleosome-based photoaffinity probe to capture proteins that recognize H3K79 dimethylation (H3K79me2) in a nucleosomal context. In combination with a quantitative proteomics approach, this probe identified menin as a H3K79me2 reader. A cryo–electron microscopy structure of menin bound to an H3K79me2 nucleosome revealed that menin engages with the nucleosome using its fingers and palm domains and recognizes the methylation mark through a π-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies. |
Persistent Identifier | http://hdl.handle.net/10722/325942 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lin, J | - |
dc.contributor.author | Wu, Y | - |
dc.contributor.author | Tian, G | - |
dc.contributor.author | Yu, D | - |
dc.contributor.author | Yang, EJ | - |
dc.contributor.author | Lam, WH | - |
dc.contributor.author | Liu, Z | - |
dc.contributor.author | Jing, Y | - |
dc.contributor.author | Dang, S | - |
dc.contributor.author | Bao, X | - |
dc.contributor.author | Wong, WHJ | - |
dc.contributor.author | Zhai, Y | - |
dc.contributor.author | Li, XD | - |
dc.date.accessioned | 2023-03-06T01:27:14Z | - |
dc.date.available | 2023-03-06T01:27:14Z | - |
dc.date.issued | 2023 | - |
dc.identifier.citation | Science, 2023, v. 379, p. 717-723 | - |
dc.identifier.uri | http://hdl.handle.net/10722/325942 | - |
dc.description.abstract | Methylation of histone H3 lysine-79 (H3K79) is an epigenetic mark for gene regulation in development, cellular differentiation, and disease progression. However, how this histone mark is translated into downstream effects remains poorly understood owing to a lack of knowledge about its readers. We developed a nucleosome-based photoaffinity probe to capture proteins that recognize H3K79 dimethylation (H3K79me2) in a nucleosomal context. In combination with a quantitative proteomics approach, this probe identified menin as a H3K79me2 reader. A cryo–electron microscopy structure of menin bound to an H3K79me2 nucleosome revealed that menin engages with the nucleosome using its fingers and palm domains and recognizes the methylation mark through a π-cation interaction. In cells, menin is selectively associated with H3K79me2 on chromatin, particularly in gene bodies. | - |
dc.language | eng | - |
dc.relation.ispartof | Science | - |
dc.title | Menin 'reads' H3K79me2 mark in a nucleosomal context | - |
dc.type | Article | - |
dc.identifier.email | Tian, G: tiangf@connect.hku.hk | - |
dc.identifier.email | Lam, WH: hl1988@hku.hk | - |
dc.identifier.email | Liu, Z: lz2021@hku.hk | - |
dc.identifier.email | Bao, X: baoxc@hku.hk | - |
dc.identifier.email | Wong, WHJ: jwhwong@hku.hk | - |
dc.identifier.email | Zhai, Y: zhai@hku.hk | - |
dc.identifier.email | Li, XD: xiangli@hku.hk | - |
dc.identifier.authority | Bao, X=rp02881 | - |
dc.identifier.authority | Wong, WHJ=rp02363 | - |
dc.identifier.authority | Zhai, Y=rp02398 | - |
dc.identifier.authority | Li, XD=rp01562 | - |
dc.identifier.doi | 10.1126/science.adc9318 | - |
dc.identifier.hkuros | 344242 | - |
dc.identifier.volume | 379 | - |
dc.identifier.spage | 717 | - |
dc.identifier.epage | 723 | - |