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Article: In vivo NIR-II structured-illumination light-sheet microscopy

TitleIn vivo NIR-II structured-illumination light-sheet microscopy
Authors
KeywordsLight-sheet microscope
Near-infrared II imaging
Noninvasive imaging
Structured-illumination microscopy
Issue Date2021
Citation
Proceedings of the National Academy of Sciences of the United States of America, 2021, v. 118, n. 6, article no. e2023888118 How to Cite?
AbstractNoninvasive optical imaging with deep tissue penetration depth and high spatiotemporal resolution is important to longitudinally studying the biology at the single-cell level in live mammals, but has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II) (1,000 to 1,700 nm) structured-illumination light-sheet microscopy (NIR-II SIM) with ultralong excitation and emission wavelengths up to ∼1,540 and ∼1,700 nm, respectively, suppressing light scattering to afford large volumetric three-dimensional (3D) imaging of tissues with deep-axial penetration depths. Integrating structured illumination into NIR-II light-sheet microscopy further diminished background and improved spatial resolution by approximately twofold. In vivo oblique NIR-II SIM was performed noninvasively for 3D volumetric multiplexed molecular imaging of the CT26 tumor microenvironment in mice, longitudinally mapping out CD4, CD8, and OX40 at the single-cell level in response to immunotherapy by cytosine-phosphate-guanine (CpG), a Toll-like receptor 9 (TLR-9) agonist combined with OX40 antibody treatment. NIR-II SIM affords an additional tool for noninvasive volumetric molecular imaging of immune cells in live mammals.
Persistent Identifierhttp://hdl.handle.net/10722/325513
ISSN
2023 Impact Factor: 9.4
2023 SCImago Journal Rankings: 3.737
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Feifei-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorZhong, Yeteng-
dc.contributor.authorSalazar, Felix-
dc.contributor.authorXu, Chun-
dc.contributor.authorRen, Fuqiang-
dc.contributor.authorQu, Liangqiong-
dc.contributor.authorWu, Anna M.-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-02-27T07:33:53Z-
dc.date.available2023-02-27T07:33:53Z-
dc.date.issued2021-
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2021, v. 118, n. 6, article no. e2023888118-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/325513-
dc.description.abstractNoninvasive optical imaging with deep tissue penetration depth and high spatiotemporal resolution is important to longitudinally studying the biology at the single-cell level in live mammals, but has been challenging due to light scattering. Here, we developed near-infrared II (NIR-II) (1,000 to 1,700 nm) structured-illumination light-sheet microscopy (NIR-II SIM) with ultralong excitation and emission wavelengths up to ∼1,540 and ∼1,700 nm, respectively, suppressing light scattering to afford large volumetric three-dimensional (3D) imaging of tissues with deep-axial penetration depths. Integrating structured illumination into NIR-II light-sheet microscopy further diminished background and improved spatial resolution by approximately twofold. In vivo oblique NIR-II SIM was performed noninvasively for 3D volumetric multiplexed molecular imaging of the CT26 tumor microenvironment in mice, longitudinally mapping out CD4, CD8, and OX40 at the single-cell level in response to immunotherapy by cytosine-phosphate-guanine (CpG), a Toll-like receptor 9 (TLR-9) agonist combined with OX40 antibody treatment. NIR-II SIM affords an additional tool for noninvasive volumetric molecular imaging of immune cells in live mammals.-
dc.languageeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.subjectLight-sheet microscope-
dc.subjectNear-infrared II imaging-
dc.subjectNoninvasive imaging-
dc.subjectStructured-illumination microscopy-
dc.titleIn vivo NIR-II structured-illumination light-sheet microscopy-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1073/pnas.2023888118-
dc.identifier.pmid33526701-
dc.identifier.scopuseid_2-s2.0-85100570876-
dc.identifier.volume118-
dc.identifier.issue6-
dc.identifier.spagearticle no. e2023888118-
dc.identifier.epagearticle no. e2023888118-
dc.identifier.eissn1091-6490-
dc.identifier.isiWOS:000617355300094-

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