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Article: In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles

TitleIn vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles
Authors
Issue Date2019
Citation
Nature Biotechnology, 2019, v. 37, n. 11, p. 1322-1331 How to Cite?
AbstractThe near-infrared-IIb (NIR-IIb) (1,500–1,700 nm) window is ideal for deep-tissue optical imaging in mammals, but lacks bright and biocompatible probes. Here, we developed biocompatible cubic-phase (α-phase) erbium-based rare-earth nanoparticles (ErNPs) exhibiting bright downconversion luminescence at ~1,600 nm for dynamic imaging of cancer immunotherapy in mice. We used ErNPs functionalized with cross-linked hydrophilic polymer layers attached to anti-PD-L1 (programmed cell death-1 ligand-1) antibody for molecular imaging of PD-L1 in a mouse model of colon cancer and achieved tumor-to-normal tissue signal ratios of ~40. The long luminescence lifetime of ErNPs (~4.6 ms) enabled simultaneous imaging of ErNPs and lead sulfide quantum dots emitting in the same ~1,600 nm window. In vivo NIR-IIb molecular imaging of PD-L1 and CD8 revealed cytotoxic T lymphocytes in the tumor microenvironment in response to immunotherapy, and altered CD8 signals in tumor and spleen due to immune activation. The cross-linked functionalization layer facilitated 90% ErNP excretion within 2 weeks without detectable toxicity in mice.
Persistent Identifierhttp://hdl.handle.net/10722/325449
ISSN
2023 Impact Factor: 33.1
2023 SCImago Journal Rankings: 18.117
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhong, Yeteng-
dc.contributor.authorMa, Zhuoran-
dc.contributor.authorWang, Feifei-
dc.contributor.authorWang, Xi-
dc.contributor.authorYang, Yijun-
dc.contributor.authorLiu, Yulai-
dc.contributor.authorZhao, Xiang-
dc.contributor.authorLi, Jiachen-
dc.contributor.authorDu, Haotian-
dc.contributor.authorZhang, Mingxi-
dc.contributor.authorCui, Qiuhong-
dc.contributor.authorZhu, Shoujun-
dc.contributor.authorSun, Qinchao-
dc.contributor.authorWan, Hao-
dc.contributor.authorTian, Ye-
dc.contributor.authorLiu, Qiang-
dc.contributor.authorWang, Weizhi-
dc.contributor.authorGarcia, K. Christopher-
dc.contributor.authorDai, Hongjie-
dc.date.accessioned2023-02-27T07:33:25Z-
dc.date.available2023-02-27T07:33:25Z-
dc.date.issued2019-
dc.identifier.citationNature Biotechnology, 2019, v. 37, n. 11, p. 1322-1331-
dc.identifier.issn1087-0156-
dc.identifier.urihttp://hdl.handle.net/10722/325449-
dc.description.abstractThe near-infrared-IIb (NIR-IIb) (1,500–1,700 nm) window is ideal for deep-tissue optical imaging in mammals, but lacks bright and biocompatible probes. Here, we developed biocompatible cubic-phase (α-phase) erbium-based rare-earth nanoparticles (ErNPs) exhibiting bright downconversion luminescence at ~1,600 nm for dynamic imaging of cancer immunotherapy in mice. We used ErNPs functionalized with cross-linked hydrophilic polymer layers attached to anti-PD-L1 (programmed cell death-1 ligand-1) antibody for molecular imaging of PD-L1 in a mouse model of colon cancer and achieved tumor-to-normal tissue signal ratios of ~40. The long luminescence lifetime of ErNPs (~4.6 ms) enabled simultaneous imaging of ErNPs and lead sulfide quantum dots emitting in the same ~1,600 nm window. In vivo NIR-IIb molecular imaging of PD-L1 and CD8 revealed cytotoxic T lymphocytes in the tumor microenvironment in response to immunotherapy, and altered CD8 signals in tumor and spleen due to immune activation. The cross-linked functionalization layer facilitated 90% ErNP excretion within 2 weeks without detectable toxicity in mice.-
dc.languageeng-
dc.relation.ispartofNature Biotechnology-
dc.titleIn vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/s41587-019-0262-4-
dc.identifier.pmid31570897-
dc.identifier.scopuseid_2-s2.0-85073967379-
dc.identifier.volume37-
dc.identifier.issue11-
dc.identifier.spage1322-
dc.identifier.epage1331-
dc.identifier.eissn1546-1696-
dc.identifier.isiWOS:000494984400020-

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