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Article: Interactions between SIRT1 and MAPK/ERK regulate neuronal apoptosis induced by traumatic brain injury in vitro and in vivo

TitleInteractions between SIRT1 and MAPK/ERK regulate neuronal apoptosis induced by traumatic brain injury in vitro and in vivo
Authors
KeywordsApoptosis
ERK
Salermide
SIRT1
Traumatic brain injury
Issue Date2012
Citation
Experimental Neurology, 2012, v. 237, n. 2, p. 489-498 How to Cite?
AbstractTraumatic brain injury (TBI) is a serious insult that frequently leads to neurological dysfunction or death. Silent information regulator family protein 1 (SIRT1), as the founding member of nicotinamide adenine dinucleotide (NAD)-dependent deacetylases, has recently been demonstrated to have neuroprotective effect in several models of neurodegenerative diseases. The present study attempts to determine whether SIRT1 has a neuroprotective effect in the model of TBI, and further to investigate the possible regulatory mechanism of neuron death. Thus, we employ transection model in vitro and weight-drop model in vivo to mimic the insults of TBI. The study shows that the expressions of SIRT1, phosphorylation extracellular signal-regulated kinase (p-ERK) and cleaved Caspase-3 are induced after trauma injury in vitro or in vivo. Furthermore, inhibiting SIRT1 by pharmacological inhibitor salermide or SIRT1 siRNA significantly promotes apoptotic neuron death and reduces ERK1/2 activation induced by mechanical injury in vitro and in vivo. Inhibition of ERK1/2 activation with PD98059 or U0126 (two mitogen activated protein kinase kinase inhibitors) in vitro and in vivo significantly attenuates the SIRT1 and cleaved Caspase-3 expression to protect neuron against TBI-induced apoptosis. These results reveal that SIRT1 plays a neuroprotective effect against neuronal apoptosis induced by TBI. The interactions between SIRT1 and MAPK/ERK pathway regulate neuronal apoptosis induced by mechanical trauma injury in vitro and in vivo. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/325246
ISSN
2023 Impact Factor: 4.6
2023 SCImago Journal Rankings: 1.552
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, Yongbo-
dc.contributor.authorLuo, Peng-
dc.contributor.authorGuo, Qindong-
dc.contributor.authorLi, Sanzhong-
dc.contributor.authorZhang, Lei-
dc.contributor.authorZhao, Mingming-
dc.contributor.authorXu, Haoxiang-
dc.contributor.authorYang, Yuefan-
dc.contributor.authorPoon, Waisang-
dc.contributor.authorFei, Zhou-
dc.date.accessioned2023-02-27T07:30:56Z-
dc.date.available2023-02-27T07:30:56Z-
dc.date.issued2012-
dc.identifier.citationExperimental Neurology, 2012, v. 237, n. 2, p. 489-498-
dc.identifier.issn0014-4886-
dc.identifier.urihttp://hdl.handle.net/10722/325246-
dc.description.abstractTraumatic brain injury (TBI) is a serious insult that frequently leads to neurological dysfunction or death. Silent information regulator family protein 1 (SIRT1), as the founding member of nicotinamide adenine dinucleotide (NAD)-dependent deacetylases, has recently been demonstrated to have neuroprotective effect in several models of neurodegenerative diseases. The present study attempts to determine whether SIRT1 has a neuroprotective effect in the model of TBI, and further to investigate the possible regulatory mechanism of neuron death. Thus, we employ transection model in vitro and weight-drop model in vivo to mimic the insults of TBI. The study shows that the expressions of SIRT1, phosphorylation extracellular signal-regulated kinase (p-ERK) and cleaved Caspase-3 are induced after trauma injury in vitro or in vivo. Furthermore, inhibiting SIRT1 by pharmacological inhibitor salermide or SIRT1 siRNA significantly promotes apoptotic neuron death and reduces ERK1/2 activation induced by mechanical injury in vitro and in vivo. Inhibition of ERK1/2 activation with PD98059 or U0126 (two mitogen activated protein kinase kinase inhibitors) in vitro and in vivo significantly attenuates the SIRT1 and cleaved Caspase-3 expression to protect neuron against TBI-induced apoptosis. These results reveal that SIRT1 plays a neuroprotective effect against neuronal apoptosis induced by TBI. The interactions between SIRT1 and MAPK/ERK pathway regulate neuronal apoptosis induced by mechanical trauma injury in vitro and in vivo. © 2012 Elsevier Inc.-
dc.languageeng-
dc.relation.ispartofExperimental Neurology-
dc.subjectApoptosis-
dc.subjectERK-
dc.subjectSalermide-
dc.subjectSIRT1-
dc.subjectTraumatic brain injury-
dc.titleInteractions between SIRT1 and MAPK/ERK regulate neuronal apoptosis induced by traumatic brain injury in vitro and in vivo-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.expneurol.2012.07.004-
dc.identifier.pmid22828134-
dc.identifier.scopuseid_2-s2.0-84865318675-
dc.identifier.volume237-
dc.identifier.issue2-
dc.identifier.spage489-
dc.identifier.epage498-
dc.identifier.eissn1090-2430-
dc.identifier.isiWOS:000309034800027-

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