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Article: Identification of two contiguous minimally deleted regions on chromosome 1p36.31-p36.32 in oligodendroglial tumours

TitleIdentification of two contiguous minimally deleted regions on chromosome 1p36.31-p36.32 in oligodendroglial tumours
Authors
KeywordsChromosome 1p
Loss of heterozygosity
Oligoastrocytoma
Oligodendroglioma
Tumour suppressor gene
Issue Date2004
Citation
British Journal of Cancer, 2004, v. 91, n. 6, p. 1105-1111 How to Cite?
AbstractLoss of the short arm of chromosome 1 is a hallmark of oligodendroglial tumours (OTs). Deletion mapping studies in OTs have revealed multiple commonly deleted regions on chromosome 1p, suggesting that there are more than one tumour suppressor gene. To map critical deletion regions on 1p, a series of 25 OTs were examined for loss of heterozygosity (LOH) on 19 polymorphic markers across the 1p arm using microsatellite analysis. Our study revealed that 60% of tumours had LOH of all informative markers on 1p and identified one tumour showing LOH at telomeric markers only. Since this deletion region lies in one of the critical deletion intervals defined previously, we then screened another series of 27 OTs specifically at 1p36.3 for LOH using nine polymorphic markers. A total of 12% (six out of 52) of tumours were found to carry interstitial deletions. The allelic status and the deletion breakpoints in these tumours with interstitial deletion were further verified by fluorescent in situ hybridisation. The small overlapping intervals facilitated the delineation of two contiguous minimally deleted regions of 0.76 Mb, defined by D1S468 and D1S2845, and of 0.41 Mb, bound by D1S2893 and D1S1608, on 1p36.31-36.32. Based on current reference human genome sequence these deletion regions have been sequenced almost to entirety and contain eight annotated genes. TP73, DFFB and SHREWI are the only known genes located in these deletion regions, while the others are uncharacterised novel genes. In conclusion, our study has narrowed down the critical tumour suppressor loci on 1p36.3, in which two minimally deleted regions are mapped, and markedly reduced the number of candidate genes to be screened for their involvement in OT development. © 2004 Cancer Research UK.
Persistent Identifierhttp://hdl.handle.net/10722/325170
ISSN
2023 Impact Factor: 6.4
2023 SCImago Journal Rankings: 3.000
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDong, Z.-
dc.contributor.authorPang, J. C.S.-
dc.contributor.authorNg, M. H.-
dc.contributor.authorPoon, W. S.-
dc.contributor.authorZhou, L.-
dc.contributor.authorNg, H. K.-
dc.date.accessioned2023-02-27T07:30:18Z-
dc.date.available2023-02-27T07:30:18Z-
dc.date.issued2004-
dc.identifier.citationBritish Journal of Cancer, 2004, v. 91, n. 6, p. 1105-1111-
dc.identifier.issn0007-0920-
dc.identifier.urihttp://hdl.handle.net/10722/325170-
dc.description.abstractLoss of the short arm of chromosome 1 is a hallmark of oligodendroglial tumours (OTs). Deletion mapping studies in OTs have revealed multiple commonly deleted regions on chromosome 1p, suggesting that there are more than one tumour suppressor gene. To map critical deletion regions on 1p, a series of 25 OTs were examined for loss of heterozygosity (LOH) on 19 polymorphic markers across the 1p arm using microsatellite analysis. Our study revealed that 60% of tumours had LOH of all informative markers on 1p and identified one tumour showing LOH at telomeric markers only. Since this deletion region lies in one of the critical deletion intervals defined previously, we then screened another series of 27 OTs specifically at 1p36.3 for LOH using nine polymorphic markers. A total of 12% (six out of 52) of tumours were found to carry interstitial deletions. The allelic status and the deletion breakpoints in these tumours with interstitial deletion were further verified by fluorescent in situ hybridisation. The small overlapping intervals facilitated the delineation of two contiguous minimally deleted regions of 0.76 Mb, defined by D1S468 and D1S2845, and of 0.41 Mb, bound by D1S2893 and D1S1608, on 1p36.31-36.32. Based on current reference human genome sequence these deletion regions have been sequenced almost to entirety and contain eight annotated genes. TP73, DFFB and SHREWI are the only known genes located in these deletion regions, while the others are uncharacterised novel genes. In conclusion, our study has narrowed down the critical tumour suppressor loci on 1p36.3, in which two minimally deleted regions are mapped, and markedly reduced the number of candidate genes to be screened for their involvement in OT development. © 2004 Cancer Research UK.-
dc.languageeng-
dc.relation.ispartofBritish Journal of Cancer-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectChromosome 1p-
dc.subjectLoss of heterozygosity-
dc.subjectOligoastrocytoma-
dc.subjectOligodendroglioma-
dc.subjectTumour suppressor gene-
dc.titleIdentification of two contiguous minimally deleted regions on chromosome 1p36.31-p36.32 in oligodendroglial tumours-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/sj.bjc.6602093-
dc.identifier.pmid15475940-
dc.identifier.pmcidPMC2747719-
dc.identifier.scopuseid_2-s2.0-5044227347-
dc.identifier.volume91-
dc.identifier.issue6-
dc.identifier.spage1105-
dc.identifier.epage1111-
dc.identifier.isiWOS:000223885200017-

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