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Article: Differential role of hydrogen peroxide and staurosporine in induction of cell death in glioblastoma cells lacking DNA-dependent protein kinase

TitleDifferential role of hydrogen peroxide and staurosporine in induction of cell death in glioblastoma cells lacking DNA-dependent protein kinase
Authors
KeywordsApoptosis
DNA-dependent protein kinase
Glioblastoma
Hydrogen peroxide and staurosporine
Necrosis
Issue Date2005
Citation
Apoptosis, 2005, v. 10, n. 1, p. 185-192 How to Cite?
AbstractVarious DNA double-strand break repair mechanisms, in which DNA-dependent protein kinase (DNA-PK) has a major role, are involved both in the development and treatment of glioblastoma. The aim of the present study was to investigate how glioblastoma cells responded to hydrogen peroxide and staurosporine (STS) and how such a response is related to DNA-PK. Two human glioblastoma cell lines, M059J cells that lack DNA-PK activity, and M059K cells that express a normal level of DNA-PK, were exposed to hydrogen peroxide or STS. The response of the cells to hydrogen peroxide or STS was recorded by measuring cell death, which was detected by three different methods-MTT, annexin-V and propidium iodide staining, and JC-1 mitochondrial probe. The result showed that both hydrogen peroxide and STS were able to induce cell death of the glioblastoma cells and that the former was mainly associated with necrosis and the latter with apoptosis. Glioblastoma cells lacking DNA-PK were less sensitive to STS treatment than those containing DNA-PK. However, DNA-PK had no significant influence on hydrogen peroxide treatment. We further found that catalase, an antioxidant enzyme, could prevent cell death induced by hydrogen peroxide but not by STS, suggesting that the pathways leading to cell death by hydrogen peroxide and STS are different. We conclude that hydrogen peroxide and STS have differential effects on cell death of glioblastoma cells lacking DNA-dependent protein kinase. Such differential roles in the induction of glioblastoma cell death can be of significant value in selecting and/or optimizing the treatment for this malignant brain tumor. © 2005 Springer Science + Business Media, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/325093
ISSN
2023 Impact Factor: 6.1
2023 SCImago Journal Rankings: 1.427
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, G. G.-
dc.contributor.authorSin, F. L.F.-
dc.contributor.authorLeung, B. C.S.-
dc.contributor.authorNg, H. K.-
dc.contributor.authorPoon, W. S.-
dc.date.accessioned2023-02-27T07:29:39Z-
dc.date.available2023-02-27T07:29:39Z-
dc.date.issued2005-
dc.identifier.citationApoptosis, 2005, v. 10, n. 1, p. 185-192-
dc.identifier.issn1360-8185-
dc.identifier.urihttp://hdl.handle.net/10722/325093-
dc.description.abstractVarious DNA double-strand break repair mechanisms, in which DNA-dependent protein kinase (DNA-PK) has a major role, are involved both in the development and treatment of glioblastoma. The aim of the present study was to investigate how glioblastoma cells responded to hydrogen peroxide and staurosporine (STS) and how such a response is related to DNA-PK. Two human glioblastoma cell lines, M059J cells that lack DNA-PK activity, and M059K cells that express a normal level of DNA-PK, were exposed to hydrogen peroxide or STS. The response of the cells to hydrogen peroxide or STS was recorded by measuring cell death, which was detected by three different methods-MTT, annexin-V and propidium iodide staining, and JC-1 mitochondrial probe. The result showed that both hydrogen peroxide and STS were able to induce cell death of the glioblastoma cells and that the former was mainly associated with necrosis and the latter with apoptosis. Glioblastoma cells lacking DNA-PK were less sensitive to STS treatment than those containing DNA-PK. However, DNA-PK had no significant influence on hydrogen peroxide treatment. We further found that catalase, an antioxidant enzyme, could prevent cell death induced by hydrogen peroxide but not by STS, suggesting that the pathways leading to cell death by hydrogen peroxide and STS are different. We conclude that hydrogen peroxide and STS have differential effects on cell death of glioblastoma cells lacking DNA-dependent protein kinase. Such differential roles in the induction of glioblastoma cell death can be of significant value in selecting and/or optimizing the treatment for this malignant brain tumor. © 2005 Springer Science + Business Media, Inc.-
dc.languageeng-
dc.relation.ispartofApoptosis-
dc.subjectApoptosis-
dc.subjectDNA-dependent protein kinase-
dc.subjectGlioblastoma-
dc.subjectHydrogen peroxide and staurosporine-
dc.subjectNecrosis-
dc.titleDifferential role of hydrogen peroxide and staurosporine in induction of cell death in glioblastoma cells lacking DNA-dependent protein kinase-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10495-005-6073-8-
dc.identifier.pmid15711934-
dc.identifier.scopuseid_2-s2.0-17644401321-
dc.identifier.volume10-
dc.identifier.issue1-
dc.identifier.spage185-
dc.identifier.epage192-
dc.identifier.isiWOS:000227070400018-

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