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Article: Inferring RNA sequence preferences for poorly studied RNA-binding proteins based on co-evolution

TitleInferring RNA sequence preferences for poorly studied RNA-binding proteins based on co-evolution
Authors
KeywordsCo-evolution
K-nearest neighbors
Machine learning
RBP binding preference
Issue Date2018
Citation
BMC Bioinformatics, 2018, v. 19, n. 1, article no. 96 How to Cite?
AbstractBackground: Characterizing the binding preference of RNA-binding proteins (RBP) is essential for us to understand the interaction between an RBP and its RNA targets, and to decipher the mechanism of post-transcriptional regulation. Experimental methods have been used to generate protein-RNA binding data for a number of RBPs in vivo and in vitro. Utilizing the binding data, a couple of computational methods have been developed to detect the RNA sequence or structure preferences of the RBPs. However, the majority of RBPs have not yet been experimentally characterized and lack RNA binding data. For these poorly studied RBPs, the identification of their binding preferences cannot be performed by most existing computational methods because the experimental binding data are prerequisite to these methods. Results: Here we propose a new method based on co-evolution to predict the sequence preferences for the poorly studied RBPs, waiving the requirement of their binding data. First, we demonstrate the co-evolutionary relationship between RBPs and their RNA partners. We then present a K-nearest neighbors (KNN) based algorithm to infer the sequence preference of an RBP using only the preference information from its homologous RBPs. By benchmarking against several in vitro and in vivo datasets, our proposed method outperforms the existing alternative which uses the closest neighbor's preference on all the datasets. Moreover, it shows comparable performance with two state-of-the-art methods that require the presence of the experimental binding data. Finally, we demonstrate the usage of this method to infer sequence preferences for novel proteins which have no binding preference information available. Conclusion: For a poorly studied RBP, the current methods used to determine its binding preference need experimental data, which is expensive and time consuming. Therefore, determining RBP's preference is not practical in many situations. This study provides an economic solution to infer the sequence preference of such protein based on the co-evolution. The source codes and related datasets are available at https://github.com/syang11/KNN.
Persistent Identifierhttp://hdl.handle.net/10722/324499
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, Shu-
dc.contributor.authorWang, Junwen-
dc.contributor.authorNg, Raymond T.-
dc.date.accessioned2023-02-03T07:03:29Z-
dc.date.available2023-02-03T07:03:29Z-
dc.date.issued2018-
dc.identifier.citationBMC Bioinformatics, 2018, v. 19, n. 1, article no. 96-
dc.identifier.urihttp://hdl.handle.net/10722/324499-
dc.description.abstractBackground: Characterizing the binding preference of RNA-binding proteins (RBP) is essential for us to understand the interaction between an RBP and its RNA targets, and to decipher the mechanism of post-transcriptional regulation. Experimental methods have been used to generate protein-RNA binding data for a number of RBPs in vivo and in vitro. Utilizing the binding data, a couple of computational methods have been developed to detect the RNA sequence or structure preferences of the RBPs. However, the majority of RBPs have not yet been experimentally characterized and lack RNA binding data. For these poorly studied RBPs, the identification of their binding preferences cannot be performed by most existing computational methods because the experimental binding data are prerequisite to these methods. Results: Here we propose a new method based on co-evolution to predict the sequence preferences for the poorly studied RBPs, waiving the requirement of their binding data. First, we demonstrate the co-evolutionary relationship between RBPs and their RNA partners. We then present a K-nearest neighbors (KNN) based algorithm to infer the sequence preference of an RBP using only the preference information from its homologous RBPs. By benchmarking against several in vitro and in vivo datasets, our proposed method outperforms the existing alternative which uses the closest neighbor's preference on all the datasets. Moreover, it shows comparable performance with two state-of-the-art methods that require the presence of the experimental binding data. Finally, we demonstrate the usage of this method to infer sequence preferences for novel proteins which have no binding preference information available. Conclusion: For a poorly studied RBP, the current methods used to determine its binding preference need experimental data, which is expensive and time consuming. Therefore, determining RBP's preference is not practical in many situations. This study provides an economic solution to infer the sequence preference of such protein based on the co-evolution. The source codes and related datasets are available at https://github.com/syang11/KNN.-
dc.languageeng-
dc.relation.ispartofBMC Bioinformatics-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCo-evolution-
dc.subjectK-nearest neighbors-
dc.subjectMachine learning-
dc.subjectRBP binding preference-
dc.titleInferring RNA sequence preferences for poorly studied RNA-binding proteins based on co-evolution-
dc.typeArticle-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s12859-018-2091-8-
dc.identifier.pmid29529991-
dc.identifier.pmcidPMC5848454-
dc.identifier.scopuseid_2-s2.0-85043479956-
dc.identifier.volume19-
dc.identifier.issue1-
dc.identifier.spagearticle no. 96-
dc.identifier.epagearticle no. 96-
dc.identifier.eissn1471-2105-
dc.identifier.isiWOS:000427156000001-

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